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AB248997

Anti-TOX3 antibody [EPR6173(2)(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TOX3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CAGF9, TNRC9, TOX3, TOX high mobility group box family member 3, CAG trinucleotide repeat-containing gene F9 protein, Trinucleotide repeat-containing gene 9 protein

2 Images
Flow Cytometry (Intracellular) - Anti-TOX3 antibody [EPR6173(2)(B)] - BSA and Azide free (AB248997)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TOX3 antibody [EPR6173(2)(B)] - BSA and Azide free (AB248997)

This data was developed using ab151548, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling TOX3 with ab151548 at 1/10 dilution (red), compared to a rabbit IgG negative control antibody (green).

Western blot - Anti-TOX3 antibody [EPR6173(2)(B)] - BSA and Azide free (AB248997)
  • WB

Unknown

Western blot - Anti-TOX3 antibody [EPR6173(2)(B)] - BSA and Azide free (AB248997)

This data was developed using ab151548, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TOX3 antibody [EPR6173(2)(B)] (<a href='/en-us/products/primary-antibodies/tox3-antibody-epr61732b-ab151548'>ab151548</a>) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

MDA-MB-435 cell lysate at 10 µg

Lane 4:

Fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6173(2)(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab248997 is the carrier-free version of ab151548.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TOX3 also known as TOX high mobility group box family member 3 is a transcription factor with a significant role in chromatin remodeling and gene expression regulation. It has a molecular mass of approximately 60 kDa. TOX3 mainly gets expressed in neuronal tissues but it is also found in breast tissue and embryonic development sites. As a transcription factor TOX3 influences cellular processes by binding to DNA and interacting with other proteins to control the transcription of target genes. Through these interactions TOX3 can activate or suppress specific genes in different contexts.
Biological function summary

TOX3 interacts with other components to influence neural development and neuroplasticity. It does not strictly operate as a lone factor; rather it associates with protein complexes for its functions. This protein plays an important part in the survival and function of neurons. In breast tissue TOX3 may influence cellular architecture and receptor signaling pathways that are important for tissue differentiation and maintenance. The dynamic role of TOX3 in various tissues marks it as a versatile regulator essential for a broad range of cellular processes.

Pathways

TOX3 integrates into the Wnt signaling and estrogen receptor pathways. These pathways are critical for developmental processes and cell fate determination. Within the Wnt signaling pathway TOX3 interacts with downstream effectors to modulate the transcriptional outcomes. In relation to the estrogen receptor pathway TOX3 links with other transcription factors such as FOXA1 to regulate target genes in hormone-responsive tissues. These interactions place TOX3 as a significant intermediary in conveying signals from external cues to drive specific cellular outcomes.

TOX3 links closely to breast cancer and neurodegenerative diseases like Alzheimer's disease. In breast cancer alterations in TOX3 expression levels correlate with disease progression and prognosis often in connection with altered functions of estrogen receptors and BRCA1. In neurodegenerative contexts TOX3 participates in pathways affecting neuronal survival and may share mechanistic links with proteins like APP linked to Alzheimer's disease pathology. The dual involvement in cancer and neurological diseases highlights TOX3 as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional coactivator of the p300/CBP-mediated transcription complex. Activates transactivation through cAMP response element (CRE) sites. Protects against cell death by inducing antiapoptotic and repressing pro-apoptotic transcripts. Stimulates transcription from the estrogen-responsive or BCL-2 promoters. Required for depolarization-induced transcription activation of the C-FOS promoter in neurons. Associates with chromatin to the estrogen-responsive C3 promoter region.
See full target information TOX3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang
View all publications
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