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AB212414

Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free

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(2 Publications)

Mouse Monoclonal TOX3 antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TOX3 aa 250-400.

View Alternative Names

CAGF9, TNRC9, TOX3, TOX high mobility group box family member 3, CAG trinucleotide repeat-containing gene F9 protein, Trinucleotide repeat-containing gene 9 protein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (AB212414)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (AB212414)

Immunohistochemical analysis of formalin fixed paraffin embedded Human Gall bladder labeling TOX3 with ab212414 at 1 μg/ml

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (AB212414)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (AB212414)

Immunohistochemical analysis of formalin fixed paraffin embedded human Cervical carcinoma labeling TOX3 with ab212414 at 1 μg/ml

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (AB212414)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (AB212414)

Immunohistochemical analysis of formalin fixed paraffin embedded Human gastric carcinoma labeling TOX3 with ab212414 at 1 μg/ml

Protein Array - Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (AB212414)
  • Protein Array

Unknown

Protein Array - Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (AB212414)

This data was produced with ab216614, the same antibody in a different formulation with BSA and Azide.
ab216614 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TOX3/1123

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Protein Array

applications

Immunogen

Recombinant Fragment Protein within Human TOX3 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

O15405

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TOX3 also known as TOX high mobility group box family member 3 is a transcription factor with a significant role in chromatin remodeling and gene expression regulation. It has a molecular mass of approximately 60 kDa. TOX3 mainly gets expressed in neuronal tissues but it is also found in breast tissue and embryonic development sites. As a transcription factor TOX3 influences cellular processes by binding to DNA and interacting with other proteins to control the transcription of target genes. Through these interactions TOX3 can activate or suppress specific genes in different contexts.
Biological function summary

TOX3 interacts with other components to influence neural development and neuroplasticity. It does not strictly operate as a lone factor; rather it associates with protein complexes for its functions. This protein plays an important part in the survival and function of neurons. In breast tissue TOX3 may influence cellular architecture and receptor signaling pathways that are important for tissue differentiation and maintenance. The dynamic role of TOX3 in various tissues marks it as a versatile regulator essential for a broad range of cellular processes.

Pathways

TOX3 integrates into the Wnt signaling and estrogen receptor pathways. These pathways are critical for developmental processes and cell fate determination. Within the Wnt signaling pathway TOX3 interacts with downstream effectors to modulate the transcriptional outcomes. In relation to the estrogen receptor pathway TOX3 links with other transcription factors such as FOXA1 to regulate target genes in hormone-responsive tissues. These interactions place TOX3 as a significant intermediary in conveying signals from external cues to drive specific cellular outcomes.

TOX3 links closely to breast cancer and neurodegenerative diseases like Alzheimer's disease. In breast cancer alterations in TOX3 expression levels correlate with disease progression and prognosis often in connection with altered functions of estrogen receptors and BRCA1. In neurodegenerative contexts TOX3 participates in pathways affecting neuronal survival and may share mechanistic links with proteins like APP linked to Alzheimer's disease pathology. The dual involvement in cancer and neurological diseases highlights TOX3 as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional coactivator of the p300/CBP-mediated transcription complex. Activates transactivation through cAMP response element (CRE) sites. Protects against cell death by inducing antiapoptotic and repressing pro-apoptotic transcripts. Stimulates transcription from the estrogen-responsive or BCL-2 promoters. Required for depolarization-induced transcription activation of the C-FOS promoter in neurons. Associates with chromatin to the estrogen-responsive C3 promoter region.
See full target information TOX3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 46:1074-1088 PubMed35347804

2022

TOX3 regulates the proliferation and apoptosis of colorectal cancer by downregulating RhoB via the activation of the MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Wei Wu,Hailiang Liang,Jiejing Chen,Xiaoqiang Dong

Frontiers in immunology 11:1466 PubMed32765508

2020

A 34-Marker Panel for Imaging Mass Cytometric Analysis of Human Snap-Frozen Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Guo,Vincent van Unen,Marieke E Ijsselsteijn,Laura F Ouboter,Andrea E van der Meulen,Susana M Chuva de Sousa Lopes,Noel F C C de Miranda,Frits Koning,Na Li
View all publications
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