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AB9775

Anti-TP53INP1 antibody

1

(1 Review)

|

(8 Publications)

Rabbit Polyclonal TP53INP1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human TP53INP1 aa 1-50.

View Alternative Names

P53DINP1, SIP, TP53INP1, Tumor protein p53-inducible nuclear protein 1, Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody (AB9775)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody (AB9775)

Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labelingp53 DINP1/TP53INP1 with Anti-p53 DINP1/TP53INP1 antibody (ab9775) at 5μg/ml.

Western blot - Anti-TP53INP1 antibody (AB9775)
  • WB

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Western blot - Anti-TP53INP1 antibody (AB9775)

Western blot analysis of p53DINP1 expression in human lung tissue lysate with anti-p53DINP1 (ab9775) at 2 μg/ml in the absence (lane 1) or presence (lane 2) of blocking peptide (ab9776). Western blot analysis of p53DINP1 expression in human lung tissue lysate with anti-p53DINP1 (ab9775) at 2 µg/ml in the absence (lane 1) or presence (lane 2) of blocking peptide (ab9776).

All lanes:

Western blot - Anti-TP53INP1 antibody (ab9775)

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Western blot - Anti-TP53INP1 antibody (AB9775)
  • WB

CiteAb

Western blot - Anti-TP53INP1 antibody (AB9775)

TP53INP1 western blot using anti-TP53INP1 antibody ab9775. Publication image and figure legend from Jiang, F., Liu, T., et al., 2011, BMC Cancer, PubMed 21970405.

ab9775 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab9775 please see the product overview.

MiR-125b negatively regulated TP53INP1 protein levels in EC cells. A. Putative binding sites of miR-125b in the TP53INP1 3'UTR (white sequences) predicted by TargetScan. B. TP53INP1 protein levels were measured in EC cells at 72 h post-transfection by western blot assays. C. Potential binding site of miR-125b on TP53INP1 3'UTR in different species. The seed sequence is underlined. D. Sketch of the construction of pMIR-TP53INP1-3'UTR-WT or pMIR-TP53INP1-3'UTR-MT vectors. The mutant binding site is underlined and italicized. E. MiR-125bm down-regulated luciferase activities controlled by wild-type TP53INP1 3'UTR, but did not affect luciferase activity controlled by mutant TP53INP1 3'UTR (white sequences were mutated). The results are means of three independent experiments ± SD. (**P < 0.01 vs. blank; #P > 0.05 vs. blank).

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TP53INP1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q96A56

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>Human lung tissue lysate can be used as a positive control and a band at 27 kDa can be detected. A lower band at 18 kDa was detected in human spleen, tissue lysates.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC.
See full target information TP53INP1

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Experimental eye research 199:108185 PubMed32841649

2020

Long non-coding RNA MEG3 promotes cataractogenesis by upregulating TP53INP1 expression in age-related cataract.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Tu,Laiqing Xie,Lili Chen,You Yuan,Bai Qin,Kun Wang,Qiujian Zhu,Na Ji,Manhui Zhu,Huaijin Guan

Experimental cell research 383:111543 PubMed31374207

2019

Exosomal miRNA-106b from cancer-associated fibroblast promotes gemcitabine resistance in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Fang,Wentao Zhou,Yefei Rong,Tiantao Kuang,Xuefeng Xu,Wenchuan Wu,Dansong Wang,Wenhui Lou

Molecular medicine reports 11:1200-6 PubMed25369914

2014

MicroRNA-96 promotes the proliferation of colorectal cancer cells and targets tumor protein p53 inducible nuclear protein 1, forkhead box protein O1 (FOXO1) and FOXO3a.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gao,Wenhui Wang

International journal of clinical and experimental 7:602-10 PubMed24551280

2014

microRNA-155 acts as an oncogene by targeting the tumor protein 53-induced nuclear protein 1 in esophageal squamous cell carcinoma.

Applications

WB, IHC-P

Species

Human, Human

Jie Zhang,Chen Cheng,Xiang Yuan,Jiang-Tu He,Qiu-Hui Pan,Fen-Yong Sun

Investigative ophthalmology & visual science 54:3806-14 PubMed23661372

2013

Overexpression of SIRT1 promotes high glucose-attenuated corneal epithelial wound healing via p53 regulation of the IGFBP3/IGF-1R/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Wang,Xiaowen Zhao,Daling Shi,Peng Chen,Yang Yu,Lingling Yang,Lixin Xie

Nucleic acids research 41:2239-54 PubMed23303785

2013

A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes.

Applications

WB

Species

Unspecified reactive species

Damon Polioudakis,Akshay A Bhinge,Patrick J Killion,Bum-Kyu Lee,Nathan S Abell,Vishwanath R Iyer

BMC cancer 11:425 PubMed21970405

2011

MiR-125b promotes proliferation and migration of type II endometrial carcinoma cells through targeting TP53INP1 tumor suppressor in vitro and in vivo.

Applications

WB

Species

Human

Feizhou Jiang,Te Liu,Yinyan He,Qin Yan,Xiaoyue Chen,Hui Wang,Xiaoping Wan

Human molecular genetics 21:196-207 PubMed21965303

2011

Survival of pancreatic beta cells is partly controlled by a TCF7L2-p53-p53INP1-dependent pathway.

Applications

WB

Species

Rat

Yuedan Zhou,Enming Zhang,Christine Berggreen,Xingjun Jing,Peter Osmark,Stefan Lang,Corrado M Cilio,Olga Göransson,Leif Groop,Erik Renström,Ola Hansson
View all publications

Product promise

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