JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB202026

Anti-TP53INP1 antibody [EPR17974]

4

(1 Review)

|

(24 Publications)

Rabbit Recombinant Monoclonal TP53INP1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 24 publications.

View Alternative Names

P53DINP1, SIP, TP53INP1, Tumor protein p53-inducible nuclear protein 1, Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-TP53INP1 antibody [EPR17974] (AB202026)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TP53INP1 antibody [EPR17974] (AB202026)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling p53 DINP1/TP53INP1 with ab202026 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing nuclear staining on HepG2 cells.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab202026 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody [EPR17974] (AB202026)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody [EPR17974] (AB202026)

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling p53 DINP1/TP53INP1 with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasm and nucleus staining on cancer cells of Human colonic adenocarcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody [EPR17974] (AB202026)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody [EPR17974] (AB202026)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling p53 DINP1/TP53INP1 with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasm staining on epithelial cells of mouse kidney tubules is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody [EPR17974] (AB202026)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody [EPR17974] (AB202026)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling p53 DINP1/TP53INP1 with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasm and weak nucleus staining on epithelial cells of rat kidney tubules is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-TP53INP1 antibody [EPR17974] (AB202026)
  • WB

Supplier Data

Western blot - Anti-TP53INP1 antibody [EPR17974] (AB202026)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-TP53INP1 antibody [EPR17974] (ab202026) at 1/10000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Exposure time: 30s

Western blot - Anti-TP53INP1 antibody [EPR17974] (AB202026)
  • WB

Supplier Data

Western blot - Anti-TP53INP1 antibody [EPR17974] (AB202026)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-TP53INP1 antibody [EPR17974] (ab202026) at 1/10000 dilution

All lanes:

Human pancreas lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Exposure time: 30s

Western blot - Anti-TP53INP1 antibody [EPR17974] (AB202026)
  • WB

Supplier Data

Western blot - Anti-TP53INP1 antibody [EPR17974] (AB202026)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TP53INP1 antibody [EPR17974] (ab202026) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Exposure time: 30s

  • Carrier free

    Anti-TP53INP1 antibody [EPR17974] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17974

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB9775

Anti-TP53INP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TP53INP1 antibody (AB9775)

  • 1
  • 1

Complementary Products

Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB32199

Anti-PTEN antibody [Y184]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-PTEN antibody [Y184] (AB32199)

  • 5
  • 15
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/300", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC.
See full target information TP53INP1
style
left-column

Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 45:806-816 PubMed40810226

2025

Exploration of the mechanism of Danggui Buxue decoction for the treatment of gastric ulcer based on network pharmacology, molecular docking, and experiment.

Applications

Unspecified application

Species

Unspecified reactive species

Song Mingming,Men Bo,Chen Mei,Liu Rui,M O Hongping,Zhang Da,Pan Tao,Wen Xudong

European journal of medical research 30:719 PubMed40770743

2025

Development of a PPP1R14B-associated immune prognostic model for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ligang Zhao,Xiaoling Jin,Qiushi Yu,Yigang He,Facai Yang,Weihu Ma,Zhengqing Lei,Jiahua Zhou

Cell biology and toxicology 41:60 PubMed40111649

2025

Olfactory mucosal mesenchymal stem cell-derived exosome Lnc A2M-AS1 ameliorates oxidative stress by regulating TP53INP1-mediated mitochondrial autophagy through interacting with IGF2BP1 in Parkinson's diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangshan Zhang,Chuang Wang,Guoshuai Yang,Yanhui Zhou,Dan Hou,Ying Xia

Scientific reports 15:5049 PubMed39934637

2025

A novel lncRNA enhances autophagy to suppress extracellular matrix via modulating TP53INP1 in human trabecular meshwork cells under oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjun Tie,Junhong Guo,Yijia Huang,Zihan Huang,Zhichao Yan,Jiemei Yuan,Xiaoli Shen,Jiantao Wang

Cell division 19:30 PubMed39487468

2024

miR-3154 promotes glioblastoma proliferation and metastasis via targeting TP53INP1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangdan Lin,Qiong Wu,Wei Lei,Dongyang Wu,Jianchun Sheng,Guobiao Liang,Guojun Hou,Di Fan

Molecular therapy. Nucleic acids 35:102221 PubMed38868363

2024

LNA-i-miR-221 activity in colorectal cancer: A reverse translational investigation.

Applications

Unspecified application

Species

Unspecified reactive species

Asad Ali,Katia Grillone,Serena Ascrizzi,Giulio Caridà,Lucia Fiorillo,Domenico Ciliberto,Nicoletta Staropoli,Pierosandro Tagliaferri,Pierfrancesco Tassone,Maria Teresa Di Martino

Non-coding RNA research 9:865-875 PubMed38586316

2024

Mir-155-5p targets TP53INP1 to promote proliferative phenotype in hypersensitivity pneumonitis lung fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Espina-Ordoñez,Yalbi Itzel Balderas-Martínez,Ana Lilia Torres-Machorro,Iliana Herrera,Mariel Maldonado,Yair Romero,Fernanda Toscano-Marquez,Annie Pardo,Moisés Selman,José Cisneros

Apoptosis : an international journal on programmed cell death 29:882-897 PubMed38491252

2024

A self-amplifying loop of TP53INP1 and P53 drives oxidative stress-induced apoptosis of bone marrow mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fanchao Li,Fei Zhang,Tao Wang,Zhihong Xie,Hong Luo,Wentao Dong,Jian Zhang,Chao Ren,Wuxun Peng

Cellular oncology (Dordrecht, Netherlands) 47:1295-1314 PubMed38436783

2024

STK3 kinase activation inhibits tumor proliferation through FOXO1-TP53INP1/P21 pathway in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ziying Zhao,Yuan Chu,Anqi Feng,Shihan Zhang,Hao Wu,Zhaoxing Li,Mingchuang Sun,Li Zhang,Tao Chen,Meidong Xu

Journal of cellular physiology 239:e31176 PubMed38179601

2024

Epitranscriptomics mA analyses reveal distinct mA marks under tumor necrosis factor α (TNF-α)-induced apoptotic conditions in HeLa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Azime Akçaöz-Alasar,Özge Tüncel,Buket Sağlam,Yasemin Gazaloğlu,Melis Atbinek,Umut Cagiral,Evin Iscan,Gunes Ozhan,Bünyamin Akgül
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com