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Rabbit Recombinant Monoclonal TPD52 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-TPD52 antibody [EPR14219] (AB181260), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14219] (AB181260), expandable thumbnail
  • Western blot - Anti-TPD52 antibody [EPR14219] (AB181260), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (AB181260), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (AB181260), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

Based on the immunogen sequence analysis, ab181260 recognizes 4 isoforms with the predicted MW of 24KDa, 20KDa, 26KDa and 22KDa, respectively.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Alternative names

Tumor protein D52, Protein N8, TPD52

Recommended products

Rabbit Recombinant Monoclonal TPD52 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14219
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Tumor Protein D52 (TPD52) is a 224 amino acid protein also known as PrLZ or D52-like protein 1 with a mass of approximately 23 kDa. TPD52 features prominently in the regulation of cell proliferation and vesicle trafficking. It is expressed in a wide range of tissues with significant expression levels in the pancreas prostate and various cancers. TPD52 associates with other members of the D52 family proteins forming homo- or heterodimers that play a part in its function.

Biological function summary

TPD52 is involved in cellular processes like growth apoptosis and exocytosis. It often forms part of protein complexes assisting in intracellular signaling pathways and vesicular transport mechanisms. The ability of TPD52 to interface with other proteins such as 14-3-3 or annexins provides it with diverse functionality. Moreover its involvement in regulating secretory pathways underlines its importance in different cell types particularly those with high secretion activity.

Pathways

TPD52 integrates into the MAPK and calcium signaling pathways. The MAPK pathway which includes key proteins like ERK1/2 involves TPD52 in processes affecting cell growth and differentiation. TPD52 can act upstream or downstream of MAPK components indicating its dynamic role in the pathway. Similarly in calcium signaling TPD52 interacts with annexins which position it within calcium-dependent exocytotic events and cellular responses dependent on Ca2+ dynamics.

Associated diseases and disorders

TPD52 shows an association with cancers such as prostate and breast cancer. In breast cancer its overexpression correlates with rapid tumor growth and decreased patient survival rates. TPD52's link to the protein AKT suggests a role in oncogenic pathways where AKT promotes cell survival and growth. Similarly in prostate cancer altered expression levels of TPD52 may enhance tumorigenic potential and aggressiveness establishing TPD52 as a potential therapeutic target for cancer treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-TPD52 antibody [EPR14219] (ab181260), expandable thumbnail

    Western blot - Anti-TPD52 antibody [EPR14219] (ab181260)

    All lanes: Western blot - Anti-TPD52 antibody [EPR14219] (ab181260) at 1/2000 dilution

    Lane 1: MCF7 cell lysate at 20 µg

    Lane 2: THP1 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14219] (ab181260), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14219] (ab181260)

    Immunocytochemistry/Immunofluorescence analysis of THP-1 cells labelling TPD52 with ab181260 at 1/1000. Cells were fixed with 100% Paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Western blot - Anti-TPD52 antibody [EPR14219] (ab181260), expandable thumbnail

    Western blot - Anti-TPD52 antibody [EPR14219] (ab181260)

    All lanes: Western blot - Anti-TPD52 antibody [EPR14219] (ab181260) at 1/5000 dilution

    All lanes: Raji cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (ab181260), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (ab181260)

    Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling TPD52 with ab181260 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (ab181260), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (ab181260)

    Immunohistochemical analysis of paraffin-embedded Human Liver tissue labeling TPD52 with ab181260 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14219] (ab181260), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14219] (ab181260)

    Intracellular flow cytometric analysis of MCF7 cells (2%paraformaldehyde-fixed) labeling TPD52 with ab181260 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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