Rabbit Recombinant Monoclonal TPD52 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples.
Images
![Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--western-blot-img144175.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56606.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629)")
![Immunoprecipitation - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--immunoprecipitation-img62670.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629)")
![Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--immunocytochemistry-immunofluorescence-img15389.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629)")
![Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--flow-cytometry-intracellular-img25538.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (AB250629)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
5 products for Alternative Version
Alternative names
Tumor protein D52, Protein N8, TPD52
Recommended products
Rabbit Recombinant Monoclonal TPD52 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14220
- Purification technique
- Affinity purification Protein A
- Specificity
Based on immunogen sequence analysis, this antibody recognizes 4 isoforms with the predicted MWs of 24KDa, 20KDa, 26 KDa and 22KDa, respectively.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250629 is the carrier-free version of Anti-TPD52 antibody [EPR14220] ab182578.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Tumor Protein D52 (TPD52) is a 224 amino acid protein also known as PrLZ or D52-like protein 1 with a mass of approximately 23 kDa. TPD52 features prominently in the regulation of cell proliferation and vesicle trafficking. It is expressed in a wide range of tissues with significant expression levels in the pancreas prostate and various cancers. TPD52 associates with other members of the D52 family proteins forming homo- or heterodimers that play a part in its function.
- Biological function summary
TPD52 is involved in cellular processes like growth apoptosis and exocytosis. It often forms part of protein complexes assisting in intracellular signaling pathways and vesicular transport mechanisms. The ability of TPD52 to interface with other proteins such as 14-3-3 or annexins provides it with diverse functionality. Moreover its involvement in regulating secretory pathways underlines its importance in different cell types particularly those with high secretion activity.
- Pathways
TPD52 integrates into the MAPK and calcium signaling pathways. The MAPK pathway which includes key proteins like ERK1/2 involves TPD52 in processes affecting cell growth and differentiation. TPD52 can act upstream or downstream of MAPK components indicating its dynamic role in the pathway. Similarly in calcium signaling TPD52 interacts with annexins which position it within calcium-dependent exocytotic events and cellular responses dependent on Ca2+ dynamics.
- Associated diseases and disorders
TPD52 shows an association with cancers such as prostate and breast cancer. In breast cancer its overexpression correlates with rapid tumor growth and decreased patient survival rates. TPD52's link to the protein AKT suggests a role in oncogenic pathways where AKT promotes cell survival and growth. Similarly in prostate cancer altered expression levels of TPD52 may enhance tumorigenic potential and aggressiveness establishing TPD52 as a potential therapeutic target for cancer treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--western-blot-img144175.jpg "Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
This data was developed using Anti-TPD52 antibody [EPR14220] ab182578, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TPD52 antibody [EPR14220] (Anti-TPD52 antibody [EPR14220] ab182578) at 1/20000 dilution
Lane 1: HCT-116 cell lysate at 10 µg
Lane 2: Raji cell lysate at 10 µg
Lane 3: THP1 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56606.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
This data was developed using Anti-TPD52 antibody [EPR14220] ab182578, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling TPD52 using Anti-TPD52 antibody [EPR14220] ab182578 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--immunoprecipitation-img62670.jpg "Immunoprecipitation - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
Immunoprecipitation - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
This data was developed using Anti-TPD52 antibody [EPR14220] ab182578, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HCT-116 cell lysate (+) or a negative control (-) immunoprecipitated using Anti-TPD52 antibody [EPR14220] ab182578 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.All lanes: Immunoprecipitation - Anti-TPD52 antibody [EPR14220] (Anti-TPD52 antibody [EPR14220] ab182578)
Predicted band size: 24 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--immunocytochemistry-immunofluorescence-img15389.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
This data was developed using Anti-TPD52 antibody [EPR14220] ab182578, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling TPD52 using Anti-TPD52 antibody [EPR14220] ab182578 at 1/500 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
![Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--flow-cytometry-intracellular-img25538.jpg "Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
This data was developed using Anti-TPD52 antibody [EPR14220] ab182578, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) cells labeling TPD52 (red) with Anti-TPD52 antibody [EPR14220] ab182578 at a 1/350 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
![Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--western-blot-img81960.jpg "Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
This data was developed using Anti-TPD52 antibody [EPR14220] ab182578, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TPD52 antibody [EPR14220] (Anti-TPD52 antibody [EPR14220] ab182578) at 1/10000 dilution
All lanes: SW480 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135720.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
This data was developed using Anti-TPD52 antibody [EPR14220] ab182578, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Prostatic hyperplasia tissue labeling TPD52 using Anti-TPD52 antibody [EPR14220] ab182578 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--western-blot-img81960.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629), expandable thumbnail](https://content.abcam.com/products/images/tpd52-antibody-epr14220-bsa-and-azide-free-ab250629--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135720.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com