AB194938

Anti-TPD52L2 antibody

Name: Anti-TPD52L2 antibody (ab194938) | Abcam
Brand: Abcam Limited
SKU: AB194938
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal TPD52L2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TPD52L2 aa 150 to C-terminus.

View Alternative Names

Tumor protein D54, hD54, Tumor protein D52-like 2, TPD52L2

2 Images

Supplier Data

Immunoprecipitation - Anti-TPD52L2 antibody (AB194938)

Detection of Human TPD52L2 by Western Blot of Immunoprecipitates.

Lane 1 : Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies : ab194938 used for IP at 6 μg per reaction. For blotting ab194938 was used at 1 μg/ml.

Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-TPD52L2 antibody (ab194938)

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-TPD52L2 antibody (AB194938)

All lanes:

Western blot - Anti-TPD52L2 antibody (ab194938) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 22 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human TPD52L2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43399

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab194938 was affinity purified using an epitope specific to TPD52L2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein TPD52L2 also known as Tumor Protein D52-like 2 and PrLZ plays a role in intracellular transport and secretion by interacting with membranes and acting as a vesicle-associated protein. It has a molecular weight of approximately 24 kDa. TPD52L2 gets highly expressed in several tissues including pancreas prostate and hematopoietic system. Moreover TPD52L2 localizes in cytoplasm and associates with secretory granules and lysosomal membranes.

Biological function summary

TPD52L2 contributes to processes such as cell proliferation and survival. It is associated with the regulation of exocytosis because of its presence in vesicles. TPD52L2 forms part of a broader protein family with conserved coiled-coil domains suggesting a capacity for protein-protein interactions. This family can affect the organization and dynamics of the cytoskeleton which is important for cytokinesis and endocytosis.

Pathways

TPD52L2 interacts directly with cellular communication and transport pathways. It is known to participate in the tumorigenesis pathway by modulating cell growth and apoptosis. The protein has interactions with key components such as PKB/AKT an important player in signaling cascades that regulate cell survival and metabolism. As a part of these pathways TPD52L2 may contribute to oncogenic transformation.

TPD52L2 has implications in cancer and metabolic disorders. Elevated levels of TPD52L2 have been linked to prostate cancer development where it interacts with proteins like FAK which are important for cancer cell migration and spread. Additionally altered expression of TPD52L2 could impact insulin signaling pathways contributing to conditions such as diabetes. Here its association with IRS proteins further illustrates the impact TPD52L2 may have within these pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information TPD52L2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 7:1 PubMed30697423

2019

The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxian Zhuang,Reynold C Ly,Carleigh V Frazier,Jia Yu,Sisi Qin,Xiao-Yang Fan,Matthew P Goetz,Judy C Boughey,Richard Weinshilboum,Liewei Wang

Experimental and therapeutic medicine 15:473-478 PubMed29375699

2018

microRNA-503 suppresses the migration, proliferation and colony formation of prostate cancer cells by targeting tumor protein D52 like 2.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Chi,Feng Ding,Wenjie Zhang,Lifa Du

American journal of translational research 9:3336-3344 PubMed28804551

2017

A tumor-suppressive microRNA, miRNA-485-5p, inhibits glioma cell proliferation and invasion by down-regulating TPD52L2.

Applications

Species

Human

Jin Yu,Shi-Wen Wu,Wei-Ping Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TPD52L2 antibody

Immunoprecipitation - Anti-TPD52L2 antibody (AB194938)

Western blot - Anti-TPD52L2 antibody (AB194938)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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