AB184178

Anti-TPGS1 antibody [EPR14324]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal TPGS1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

C19orf20, TPGS1, Tubulin polyglutamylase complex subunit 1, PGs1

4 Images

Flow Cytometry (Intracellular) - Anti-TPGS1 antibody [EPR14324] (AB184178)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TPGS1 antibody [EPR14324] (AB184178)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed SH-SY5Y cells staining TPGS1 using ab184178 at 1/50 and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve), and a Rabbit monoclonal IgG negative control (green curve).

Supplier Data

Immunoprecipitation - Anti-TPGS1 antibody [EPR14324] (AB184178)

Immunoprecipitation analysis of SH-SY5Y cells labeling TPGS1 using ab184178 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-TPGS1 antibody [EPR14324] (ab184178)

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-TPGS1 antibody [EPR14324] (AB184178)

All lanes:

Western blot - Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/2000 dilution

Lane 1:

Rat testis tissue lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-TPGS1 antibody [EPR14324] (AB184178)

All lanes:

Western blot - Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/10000 dilution

All lanes:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 31 kDa

false

Carrier free

Anti-TPGS1 antibody [EPR14324] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14324

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPGS1 also known as TPR Glycosylation Sensor 1 functions primarily as a sensor and regulator of glycosylation within the cell. Its molecular mass is approximately 85 kDa and it predominantly localizes in the endoplasmic reticulum membrane where it plays an important role in monitoring N-linked glycosylation processes. TPGS1 is expressed in various tissues showing higher levels in organs like the liver and pancreas which are integral to metabolism and detoxification processes.

Biological function summary

The TPGS1 protein interacts with glycoproteins to ensure correct folding and function important for maintaining cellular homeostasis. It frequently operates in association with complexes involved in protein processing particularly those related to the calnexin/calreticulin cycle. TPGS1 contributes to quality control by detecting improperly glycosylated proteins directing them for degradation to prevent accumulation of defective proteins which could disrupt cellular function.

Pathways

TPGS1 plays important roles in the unfolded protein response (UPR) and the endoplasmic reticulum-associated degradation (ERAD) pathways. Within these pathways TPGS1 works closely with proteins such as BiP/GRP78 and EDEM which facilitate the identification and disposal of misfolded glycoproteins. By modulating protein folding and degradation TPGS1 helps maintain ER function and prevent stress conditions that could affect the entire cellular network.

TPGS1 has connections with neurodegenerative diseases and congenital disorders of glycosylation. Mismanagement of glycoproteins due to TPGS1 malfunction can contribute to neurodegenerative conditions where protein aggregation becomes problematic. In congenital glycosylation disorders TPGS1’s interaction with proteins like TMEM165 may lead to incorrect glycosylation patterns resulting in varied systemic symptoms. Understanding TPGS1’s role in these pathological conditions may aid in developing potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit of the tubulin polyglutamylase complex (TPGC). The complex mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility (Probable). May act in the targeting of the tubulin polyglutamylase complex. Required for the development of the spermatid flagellum (By similarity).

See full target information TPGS1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical science (London, England : 1979) 139: PubMed39866066

2025

Blockade of neddylation through targeted inhibition of DCN1 alleviates renal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Ling Huo,Wenjia Fu,Qi Feng,Shaokang Pan,Dongwei Liu,Zhangsuo Liu

FEBS open bio 15:151-164 PubMed39482856

2024

Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuma Yoshioka,Reiko Nakagawa,Chi Lieu Kim Nguyen,Hayate Suzuki,Kiyohiro Ishigaki,Seiya Mizuno,Tsukasa Okiyoneda,Shizufumi Ebihara,Kazuya Murata

Cell research 32:190-209 PubMed34782749

2021

Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Sharad C Paudyal,Yuchen Kang,Mikito Owa,Feng-Xia Liang,Alexander Spektor,Holger Knaut,Irma Sánchez,Brian D Dynlacht

Cell 184:3022-3040.e28 PubMed33961781

2021

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

Applications

Unspecified application

Species

Unspecified reactive species

Edward L Huttlin,Raphael J Bruckner,Jose Navarrete-Perea,Joe R Cannon,Kurt Baltier,Fana Gebreab,Melanie P Gygi,Alexandra Thornock,Gabriela Zarraga,Stanley Tam,John Szpyt,Brandon M Gassaway,Alexandra Panov,Hannah Parzen,Sipei Fu,Arvene Golbazi,Eila Maenpaa,Keegan Stricker,Sanjukta Guha Thakurta,Tian Zhang,Ramin Rad,Joshua Pan,David P Nusinow,Joao A Paulo,Devin K Schweppe,Laura Pontano Vaites,J Wade Harper,Steven P Gygi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TPGS1 antibody [EPR14324]

Flow Cytometry (Intracellular) - Anti-TPGS1 antibody [EPR14324] (AB184178)

Immunoprecipitation - Anti-TPGS1 antibody [EPR14324] (AB184178)

Western blot - Anti-TPGS1 antibody [EPR14324] (AB184178)

Western blot - Anti-TPGS1 antibody [EPR14324] (AB184178)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise