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AB250733

Anti-TPGS1 antibody [EPR14324] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TPGS1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

C19orf20, TPGS1, Tubulin polyglutamylase complex subunit 1, PGs1

4 Images
Flow Cytometry (Intracellular) - Anti-TPGS1 antibody [EPR14324] - BSA and Azide free (AB250733)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TPGS1 antibody [EPR14324] - BSA and Azide free (AB250733)

This data was developed using ab184178, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed SH-SY5Y cells staining TPGS1 using ab184178 at 1/50 and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve), and a Rabbit monoclonal IgG negative control (green curve).

Immunoprecipitation - Anti-TPGS1 antibody [EPR14324] - BSA and Azide free (AB250733)
  • IP

Supplier Data

Immunoprecipitation - Anti-TPGS1 antibody [EPR14324] - BSA and Azide free (AB250733)

This data was developed using ab184178, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of SH-SY5Y cells labeling TPGS1 using ab184178 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-TPGS1 antibody [EPR14324] (<a href='/en-us/products/primary-antibodies/tpgs1-antibody-epr14324-ab184178'>ab184178</a>)

Predicted band size: 31 kDa

false

Western blot - Anti-TPGS1 antibody [EPR14324] - BSA and Azide free (AB250733)
  • WB

Supplier Data

Western blot - Anti-TPGS1 antibody [EPR14324] - BSA and Azide free (AB250733)

This data was developed using ab184178, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TPGS1 antibody [EPR14324] (<a href='/en-us/products/primary-antibodies/tpgs1-antibody-epr14324-ab184178'>ab184178</a>) at 1/2000 dilution

Lane 1:

Rat testis tissue lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 31 kDa

false

Western blot - Anti-TPGS1 antibody [EPR14324] - BSA and Azide free (AB250733)
  • WB

Supplier Data

Western blot - Anti-TPGS1 antibody [EPR14324] - BSA and Azide free (AB250733)

This data was developed using ab184178, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TPGS1 antibody [EPR14324] (<a href='/en-us/products/primary-antibodies/tpgs1-antibody-epr14324-ab184178'>ab184178</a>) at 1/10000 dilution

All lanes:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14324

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250733 is the carrier-free version of ab184178.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPGS1 also known as TPR Glycosylation Sensor 1 functions primarily as a sensor and regulator of glycosylation within the cell. Its molecular mass is approximately 85 kDa and it predominantly localizes in the endoplasmic reticulum membrane where it plays an important role in monitoring N-linked glycosylation processes. TPGS1 is expressed in various tissues showing higher levels in organs like the liver and pancreas which are integral to metabolism and detoxification processes.
Biological function summary

The TPGS1 protein interacts with glycoproteins to ensure correct folding and function important for maintaining cellular homeostasis. It frequently operates in association with complexes involved in protein processing particularly those related to the calnexin/calreticulin cycle. TPGS1 contributes to quality control by detecting improperly glycosylated proteins directing them for degradation to prevent accumulation of defective proteins which could disrupt cellular function.

Pathways

TPGS1 plays important roles in the unfolded protein response (UPR) and the endoplasmic reticulum-associated degradation (ERAD) pathways. Within these pathways TPGS1 works closely with proteins such as BiP/GRP78 and EDEM which facilitate the identification and disposal of misfolded glycoproteins. By modulating protein folding and degradation TPGS1 helps maintain ER function and prevent stress conditions that could affect the entire cellular network.

TPGS1 has connections with neurodegenerative diseases and congenital disorders of glycosylation. Mismanagement of glycoproteins due to TPGS1 malfunction can contribute to neurodegenerative conditions where protein aggregation becomes problematic. In congenital glycosylation disorders TPGS1’s interaction with proteins like TMEM165 may lead to incorrect glycosylation patterns resulting in varied systemic symptoms. Understanding TPGS1’s role in these pathological conditions may aid in developing potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the tubulin polyglutamylase complex (TPGC). The complex mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility (Probable). May act in the targeting of the tubulin polyglutamylase complex. Required for the development of the spermatid flagellum (By similarity).
See full target information TPGS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 24:1541-1557 PubMed36192632

2022

Alternative RNA splicing modulates ribosomal composition and determines the spatial phenotype of glioblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tatyana D Larionova,Soniya Bastola,Tatiana E Aksinina,Ksenia S Anufrieva,Jia Wang,Victoria O Shender,Dmitriy E Andreev,Tatiana F Kovalenko,Georgij P Arapidi,Polina V Shnaider,Anastasia N Kazakova,Yaroslav A Latyshev,Victor V Tatarskiy,Alexander A Shtil,Pascale Moreau,Francis Giraud,Chaoxi Li,Yichan Wang,Maria P Rubtsova,Olga A Dontsova,Michael Condro,Benjamin M Ellingson,Mikhail I Shakhparonov,Harley I Kornblum,Ichiro Nakano,Marat S Pavlyukov
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com