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AB111828

Anti-TPH2 antibody

5

(1 Review)

|

(23 Publications)

Rabbit Polyclonal TPH2 antibody. Suitable for IP and reacts with Rat samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human TPH2 aa 1-50.

View Alternative Names

NTPH, TPH2, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

1 Images
Immunoprecipitation - Anti-TPH2 antibody (AB111828)
  • IP

Supplier Data

Immunoprecipitation - Anti-TPH2 antibody (AB111828)

Immunoprecipitation analysis of Raphe Nuclei isolated from Sprague-Dawley, precipitating ab111828 coupled to magnetic beads, which was introduced to whole cell extracts at room temperature for 15 mins. Immunoprecipitated proteins were assessed by Western blotting.

Lane 1 (Positive Control) : Non-immunoprecipitated brain lysates were probed with ab111828.
Lane 2 (ab111828 Ms TPH2) : Immunoprecipitated Proteins
Lane 3 (Sheep TPH2 antibody) : Immunoprecipitated Proteins
Lane 4 (Negative Control => Anti-Tyrosine Hydroxylase antibody) : Immunoprecipitated proteins

All lanes:

Immunoprecipitation - Anti-TPH2 antibody (ab111828)

Predicted band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IP

applications

Immunogen

Synthetic Peptide within Human TPH2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8IWU9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.1% Sodium azide Constituents: 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TPH2 protein also known as Tryptophan Hydroxylase 2 acts as a catalyst in the rate-limiting step of serotonin biosynthesis. It resides mostly in the brain specifically within neurons of the central nervous system where it facilitates the conversion of tryptophan to 5-hydroxytryptophan. TPH2 has a molecular mass of approximately 55 kDa. TPH2 expression is mainly observed in the raphe nuclei a region involved in mood regulation and other neural functions.
Biological function summary

TPH2 is essential for serotonin production impacting mood cognition and neurodevelopment. It functions independently rather than as part of a complex. By controlling serotonin levels TPH2 influences many physiological processes including sleep cycles appetite and emotional status. Its activity is important for maintaining normal levels of serotonin in the synaptic cleft therefore contributing to overall neural communication.

Pathways

TPH2 is involved in the serotonin biosynthesis pathway where it regulates serotonin production. This pathway is vital for mood and emotional regulation. TPH2 also interacts with enzymes like AADC (Aromatic L-amino acid decarboxylase) which further converts 5-hydroxytryptophan into serotonin. These interactions ensure a balanced production of serotonin essential for proper neuronal signaling and stable mental states.

TPH2 has connections with mental health conditions such as depression and anxiety disorders. Altered TPH2 function can lead to reduced serotonin levels contributing to the pathophysiology of these disorders. Studies show that TPH2 may interact with proteins like MAOA (Monoamine oxidase A) where mutations or dysregulation can exacerbate mood disorders. Understanding TPH2's role offers insights into therapeutic strategies targeting serotonin imbalances in mental health conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TPH2
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Neurochemical research 49:3277-3296 PubMed39266897

2024

Neuromedin U Neurons in the Edinger-Westphal Nucleus Respond to Alcohol Without Interfering with the Urocortin 1 Response.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Medrano,Wissal Allaoui,Ra'fat Ehab Salim Haddad,Leila Makrini-Maleville,Emmanuel Valjent,Ilse Smolders,Viktória Kormos,Balázs Gaszner,Dimitri De Bundel

Acta physiologica (Oxford, England) 240:e14198 PubMed38958443

2024

Serotonergic input from the dorsal raphe nucleus shapes learning-associated odor responses in the olfactory bulb.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Jing,Chi Geng,Penglai Liu,Dejuan Wang,Qun Li,Anan Li

Research (Washington, D.C.) 7:0400 PubMed38939042

2024

Brain Short-Chain Fatty Acids Induce ACSS2 to Ameliorate Depressive-Like Behavior via PPARγ-TPH2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Nuo Chen,Xinyi Xu,Yaxin Guo,Ming Zhao,Yubin Li,Tian Zhou,Xinyue Zhang,Jie Gao,Faliang Zhu,Chun Guo,Yongyu Shi,Qun Wang,Wenxian Wu,Lining Zhang,Yan Li

Science advances 10:eadl2675 PubMed38941473

2024

Neural circuits expressing the serotonin 2C receptor regulate memory in mice and humans.

Applications

Unspecified application

Species

Unspecified reactive species

Hesong Liu,Yang He,Hailan Liu,Bas Brouwers,Na Yin,Katherine Lawler,Julia M Keogh,Elana Henning,Dong-Kee Lee,Meng Yu,Longlong Tu,Nan Zhang,Kristine M Conde,Junying Han,Zili Yan,Nikolas A Scarcelli,Lan Liao,Jianming Xu,Qingchun Tong,Hui Zheng,Zheng Sun,Yongjie Yang,Chunmei Wang,Yanlin He,I Sadaf Farooqi,Yong Xu

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38897723

2024

A Light-Responsive Neural Circuit Suppresses Feeding.

Applications

Unspecified application

Species

Unspecified reactive species

Hailan Liu,Na Qu,Natalia Valdez Gonzalez,Marco A Palma,Huamin Chen,Jiani Xiong,Abhinav Choubey,Yongxiang Li,Xin Li,Meng Yu,Hesong Liu,Longlong Tu,Nan Zhang,Na Yin,Kristine Marie Conde,Mengjie Wang,Jonathan Carter Bean,Junying Han,Nikolas Anthony Scarcelli,Yongjie Yang,Kenji Saito,Huxing Cui,Qingchun Tong,Zheng Sun,Chunmei Wang,Xing Cai,Li Lu,Yang He,Yong Xu

Nature communications 15:2891 PubMed38570514

2024

Dopamine control of social novelty preference is constrained by an interpeduncular-tegmentum circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Susanna Molas,Timothy G Freels,Rubing Zhao-Shea,Timothy Lee,Pablo Gimenez-Gomez,Melanie Barbini,Gilles E Martin,Andrew R Tapper

RSC advances 13:26203-26212 PubMed37671005

2023

Measuring tryptophan dynamics using fast scan cyclic voltammetry at carbon fiber microelectrodes with improved sensitivity and selectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Schapira,Margaret R O'Neill,Lillian Russo-Savage,Terdha Narla,Kathryn A Laprade,James M Stafford,Yangguang Ou

Nature communications 14:5124 PubMed37612268

2023

A glutamatergic DRN-VTA pathway modulates neuropathic pain and comorbid anhedonia-like behavior in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yue Wang,Wen-Bin Jia,Xiang Xu,Rui Chen,Liang-Biao Wang,Xiao-Jing Su,Peng-Fei Xu,Xiao-Qing Liu,Jie Wen,Xiao-Yuan Song,Yuan-Yuan Liu,Zhi Zhang,Xin-Feng Liu,Yan Zhang

Frontiers in cellular neuroscience 16:949923 PubMed35936499

2022

Differential vulnerability of locus coeruleus and dorsal raphe neurons to chronic methamphetamine-induced degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yijuan Du,Sanghoon Choi,Alexander Pilski,Steven M Graves

Laboratory investigation; a journal of technical methods and pathology 102:1023-1037 PubMed35585132

2022

5-HT receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Ying Chang,Yi-Ting Yang,Meng-Ping She,Chia-Hung Tu,Tsung-Chun Lee,Ming-Shiang Wu,Chin-Hung Sun,Ling-Wei Hsin,Linda Chia-Hui Yu
View all publications
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Product promise

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