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AB121013

Anti-TPH2 antibody

4

(4 Reviews)

|

(20 Publications)

Anti-TPH2 antibody (ab121013) is a goat polyclonal antibody detecting TPH2 in Western Blot. Suitable for Human.

- Over 10 publications

View Alternative Names

NTPH, TPH2, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

1 Images
Western blot - Anti-TPH2 antibody (AB121013)
  • WB

Unknown

Western blot - Anti-TPH2 antibody (AB121013)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-TPH2 antibody (ab121013) at 0.5 µg/mL

All lanes:

HEK293 lysate in RIPA buffer at 35 µg

Predicted band size: 56 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TPH2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8IWU9

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Reactivity data

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Product details

What is this antibody validated in?
Anti-TPH2 antibody (ab121013) is a goat polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of TPH2?
Anti-TPH2 (ab121013) specifically detects a band for TPH2 (UniProt: Q8IWU9) at a molecular weight of 56kDa.

Trusted by the scientific community
Anti-TPH2 (ab121013) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab121013 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TPH2 protein also known as Tryptophan Hydroxylase 2 acts as a catalyst in the rate-limiting step of serotonin biosynthesis. It resides mostly in the brain specifically within neurons of the central nervous system where it facilitates the conversion of tryptophan to 5-hydroxytryptophan. TPH2 has a molecular mass of approximately 55 kDa. TPH2 expression is mainly observed in the raphe nuclei a region involved in mood regulation and other neural functions.
Biological function summary

TPH2 is essential for serotonin production impacting mood cognition and neurodevelopment. It functions independently rather than as part of a complex. By controlling serotonin levels TPH2 influences many physiological processes including sleep cycles appetite and emotional status. Its activity is important for maintaining normal levels of serotonin in the synaptic cleft therefore contributing to overall neural communication.

Pathways

TPH2 is involved in the serotonin biosynthesis pathway where it regulates serotonin production. This pathway is vital for mood and emotional regulation. TPH2 also interacts with enzymes like AADC (Aromatic L-amino acid decarboxylase) which further converts 5-hydroxytryptophan into serotonin. These interactions ensure a balanced production of serotonin essential for proper neuronal signaling and stable mental states.

TPH2 has connections with mental health conditions such as depression and anxiety disorders. Altered TPH2 function can lead to reduced serotonin levels contributing to the pathophysiology of these disorders. Studies show that TPH2 may interact with proteins like MAOA (Monoamine oxidase A) where mutations or dysregulation can exacerbate mood disorders. Understanding TPH2's role offers insights into therapeutic strategies targeting serotonin imbalances in mental health conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TPH2
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Psychopharmacology 242:763-781 PubMed39903245

2025

Exploring the effects of adolescent social isolation stress on the serotonin system and ethanol-motivated behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan D McElroy,Chen Li,Nicholas S McCloskey,Amber R Alberici,Lynn G Kirby

Acta neuropathologica 148:76 PubMed39607506

2024

Raphe and ventrolateral medulla proteomics in sudden unexplained death in childhood with febrile seizure history.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique F Leitner,Christopher William,Arline Faustin,Evgeny Kanshin,Matija Snuderl,Declan McGuone,Thomas Wisniewski,Beatrix Ueberheide,Laura Gould,Orrin Devinsky

Communications biology 7:998 PubMed39147805

2024

Role of the circadian nuclear receptor REV-ERBα in dorsal raphe serotonin synthesis in mood regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Inah Park,Mijung Choi,Jeongah Kim,Sangwon Jang,Doyeon Kim,Jihoon Kim,Youngshik Choe,Dongho Geum,Seong-Woon Yu,Ji-Woong Choi,Cheil Moon,Han Kyoung Choe,Gi Hoon Son,Kyungjin Kim

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 49:1580-1589 PubMed38609530

2024

Rebound activation of 5-HT neurons following SSRI discontinuation.

Applications

Unspecified application

Species

Unspecified reactive species

Helen M Collins,L Sophie Gullino,Dersu Ozdemir,Caroline Lazarenco,Yulia Sudarikova,Elizabeth Daly,Fuencisla Pilar Cuéllar,Raquel Pinacho,David M Bannerman,Trevor Sharp

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305581 PubMed38488323

2024

Manipulation of Glutamatergic Neuronal Activity in the Primary Motor Cortex Regulates Cardiac Function in Normal and Myocardial Infarction Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Bo,Mengxin Cai,Yixuan Ma,Lingyun Di,Yanbin Geng,Hangzhuo Li,Caicai Tang,Fadao Tai,Zhixiong He,Zhenjun Tian

International journal of molecular sciences 24: PubMed37511364

2023

Involvement of Serotonergic Projections from the Dorsal Raphe to the Medial Preoptic Area in the Regulation of the Pup-Directed Paternal Response of Male Mandarin Voles.

Applications

Unspecified application

Species

Unspecified reactive species

Zijian Lv,Lu Li,Yin Li,Lizi Zhang,Xing Guo,Caihong Huang,Wenjuan Hou,Yishan Qu,Limin Liu,Yitong Li,Zhixiong He,Fadao Tai

Journal of neuroinflammation 20:68 PubMed36906584

2023

[F]F-DED PET imaging of reactive astrogliosis in neurodegenerative diseases: preclinical proof of concept and first-in-human data.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Ballweg,Carolin Klaus,Letizia Vogler,Sabrina Katzdobler,Karin Wind,Artem Zatcepin,Sibylle I Ziegler,Birkan Secgin,Florian Eckenweber,Bernd Bohr,Alexander Bernhardt,Urban Fietzek,Boris-Stephan Rauchmann,Sophia Stoecklein,Stefanie Quach,Leonie Beyer,Maximilian Scheifele,Marcel Simmet,Emanuel Joseph,Simon Lindner,Isabella Berg,Norman Koglin,Andre Mueller,Andrew W Stephens,Peter Bartenstein,Joerg C Tonn,Nathalie L Albert,Tania Kümpfel,Martin Kerschensteiner,Robert Perneczky,Johannes Levin,Lars Paeger,Jochen Herms,Matthias Brendel

Behavioural brain research 444:114363 PubMed36849047

2023

Wireless stimulation of the subthalamic nucleus with nanoparticles modulates key monoaminergic systems similar to contemporary deep brain stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Faisal Alosaimi,David Dominguez-Paredes,Rick Knoben,Faris Almasabi,Sarah Hescham,Kristen Kozielski,Yasin Temel,Ali Jahanshahi

Nature communications 13:4039 PubMed35864121

2022

Lateral habenula glutamatergic neurons projecting to the dorsal raphe nucleus promote aggressive arousal in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Aki Takahashi,Romain Durand-de Cuttoli,Meghan E Flanigan,Emi Hasegawa,Tomomi Tsunematsu,Hossein Aleyasin,Yoan Cherasse,Ken Miya,Takuya Okada,Kazuko Keino-Masu,Koshiro Mitsui,Long Li,Vishwendra Patel,Robert D Blitzer,Michael Lazarus,Kenji F Tanaka,Akihiro Yamanaka,Takeshi Sakurai,Sonoko Ogawa,Scott J Russo

Brain communications 4:fcac186 PubMed35928051

2022

Raphe and ventrolateral medulla proteomics in epilepsy and sudden unexpected death in epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique F Leitner,Evgeny Kanshin,Manor Askenazi,Arline Faustin,Daniel Friedman,Sasha Devore,Beatrix Ueberheide,Thomas Wisniewski,Orrin Devinsky
View all publications
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