Anti-TPH2 antibody (ab121013) is a goat polyclonal antibody that is used to detect TPH2 in Western Blot. Suitable for Human samples.
- Over 10 publications
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- International journal of molecular sciences 24:2023Involvement of Serotonergic Projections from the Dorsal Raphe to the Medial Preoptic Area in the Regulation of the Pup-Directed Paternal Response of Male Mandarin Voles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZijian Lv,Lu Li,Yin Li,Lizi Zhang,Xing Guo,Caihong Huang,Wenjuan Hou,Yishan Qu,Limin Liu,Yitong Li,Zhixiong He,Fadao TaiPubMed 37511364
- Journal of neuroinflammation 20:682023[F]F-DED PET imaging of reactive astrogliosis in neurodegenerative diseases: preclinical proof of concept and first-in-human data.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Ballweg,Carolin Klaus,Letizia Vogler,Sabrina Katzdobler,Karin Wind,Artem Zatcepin,Sibylle I Ziegler,Birkan Secgin,Florian Eckenweber,Bernd Bohr,Alexander Bernhardt,Urban Fietzek,Boris-Stephan Rauchmann,Sophia Stoecklein,Stefanie Quach,Leonie Beyer,Maximilian Scheifele,Marcel Simmet,Emanuel Joseph,Simon Lindner,Isabella Berg,Norman Koglin,Andre Mueller,Andrew W Stephens,Peter Bartenstein,Joerg C Tonn,Nathalie L Albert,Tania Kümpfel,Martin Kerschensteiner,Robert Perneczky,Johannes Levin,Lars Paeger,Jochen Herms,Matthias BrendelPubMed 36906584
- Nature communications 13:40392022Lateral habenula glutamatergic neurons projecting to the dorsal raphe nucleus promote aggressive arousal in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAki Takahashi,Romain Durand-de Cuttoli,Meghan E Flanigan,Emi Hasegawa,Tomomi Tsunematsu,Hossein Aleyasin,Yoan Cherasse,Ken Miya,Takuya Okada,Kazuko Keino-Masu,Koshiro Mitsui,Long Li,Vishwendra Patel,Robert D Blitzer,Michael Lazarus,Kenji F Tanaka,Akihiro Yamanaka,Takeshi Sakurai,Sonoko Ogawa,Scott J RussoPubMed 35864121
- Brain communications 4:fcac1862022Raphe and ventrolateral medulla proteomics in epilepsy and sudden unexpected death in epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominique F Leitner,Evgeny Kanshin,Manor Askenazi,Arline Faustin,Daniel Friedman,Sasha Devore,Beatrix Ueberheide,Thomas Wisniewski,Orrin DevinskyPubMed 35928051
- eLife 10:2021Dorsal raphe nucleus to anterior cingulate cortex 5-HTergic neural circuit modulates consolation and sociability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaifu Li,Li-Zi Zhang,Zhi-Xiong He,Huan Ma,Yu-Ting Zhang,Yu-Feng Xun,Wei Yuan,Wen-Juan Hou,Yi-Tong Li,Zi-Jian Lv,Rui Jia,Fa-Dao TaiPubMed 34080539
- Progress in neurobiology 200:1019722020Evidence that corticofugal propagation of ALS pathology is not mediated by prion-like mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJelena Scekic-Zahirovic,Mathieu Fischer,Geoffrey Stuart-Lopez,Thibaut Burg,Johan Gilet,Sylvie Dirrig-Grosch,Christine Marques,Marie-Christine Birling,Pascal Kessler,Caroline RouauxPubMed 33309802
- Brain pathology (Zurich, Switzerland) 31:133-1432020Medullary tyrosine hydroxylase catecholaminergic neuronal populations in sudden unexpected death in epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSmriti Patodia,Ian Tan,Matthew Ellis,Alyma Somani,Ingrid E Scheffer,Sanjay M Sisodiya,Maria ThomPubMed 32852867
- Annals of neurology 88:688-7022020Absence of Subcerebral Projection Neurons Is Beneficial in a Mouse Model of Amyotrophic Lateral Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThibaut Burg et. al.PubMed 32588450
- Nature 567:535-5392019Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorna A Farrelly et. al.PubMed 30867594
- Neuropsychiatric disease and treatment 15:21-312018Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyan Jiao,Zhiyi Yan,Qingyu Ma,Xiaojuan Li,Youming Jiang,Yueyun Liu,Jiaxu ChenPubMed 30587994
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TPH2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IWU9
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
| Cow | Predicted |
| Cynomolgus monkey | Predicted |
| Dog | Predicted |
| Gorilla | Predicted |
| Hamster | Predicted |
| Horse | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Alternative names
NTPH, TPH2, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2
Recommended products
Anti-TPH2 antibody (ab121013) is a goat polyclonal antibody that is used to detect TPH2 in Western Blot. Suitable for Human samples.
- Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TPH2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IWU9
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab121013 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TPH2 antibody (ab121013) is a goat polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.
What is the molecular weight of TPH2?
Anti-TPH2 (ab121013) specifically detects a band for TPH2 (UniProt: Q8IWU9) at a molecular weight of 56kDa.
Trusted by the scientific community
Anti-TPH2 (ab121013) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TPH2 protein also known as Tryptophan Hydroxylase 2 acts as a catalyst in the rate-limiting step of serotonin biosynthesis. It resides mostly in the brain specifically within neurons of the central nervous system where it facilitates the conversion of tryptophan to 5-hydroxytryptophan. TPH2 has a molecular mass of approximately 55 kDa. TPH2 expression is mainly observed in the raphe nuclei a region involved in mood regulation and other neural functions.
- Biological function summary
TPH2 is essential for serotonin production impacting mood cognition and neurodevelopment. It functions independently rather than as part of a complex. By controlling serotonin levels TPH2 influences many physiological processes including sleep cycles appetite and emotional status. Its activity is important for maintaining normal levels of serotonin in the synaptic cleft therefore contributing to overall neural communication.
- Pathways
TPH2 is involved in the serotonin biosynthesis pathway where it regulates serotonin production. This pathway is vital for mood and emotional regulation. TPH2 also interacts with enzymes like AADC (Aromatic L-amino acid decarboxylase) which further converts 5-hydroxytryptophan into serotonin. These interactions ensure a balanced production of serotonin essential for proper neuronal signaling and stable mental states.
- Associated diseases and disorders
TPH2 has connections with mental health conditions such as depression and anxiety disorders. Altered TPH2 function can lead to reduced serotonin levels contributing to the pathophysiology of these disorders. Studies show that TPH2 may interact with proteins like MAOA (Monoamine oxidase A) where mutations or dysregulation can exacerbate mood disorders. Understanding TPH2's role offers insights into therapeutic strategies targeting serotonin imbalances in mental health conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-TPH2 antibody (ab121013)
Primary incubation was 1 hour. Detected by chemiluminescence.
All lanes: Western blot - Anti-TPH2 antibody (ab121013) at 0.5 µg/mL
All lanes: HEK293 lysate in RIPA buffer at 35 µg
Predicted band size: 56 kDa
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