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Goat Polyclonal TPH2 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human TPH2 aa 1-50.


Images

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human TPH2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IWU9

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Rat
Predicted
Cow
Predicted
Cynomolgus monkey
Predicted
Dog
Predicted
Gorilla
Predicted
Hamster
Predicted
Horse
Predicted
Orangutan
Predicted
Pig
Predicted
Rabbit
Predicted
Rhesus monkey
Predicted

Tested
Tested

Species

Human

Dilution info

0.5-2 µg/mL

Notes

1 hour primary incubation is recommended for this product.

Predicted
Predicted

Species

Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan

Dilution info

-

Notes

-

Alternative names

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Goat Polyclonal TPH2 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human TPH2 aa 1-50.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human TPH2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IWU9
Purification technique

Affinity purification Immunogen

Concentration
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Purification notes

ab121013 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

TPH2 is essential for serotonin production impacting mood cognition and neurodevelopment. It functions independently rather than as part of a complex. By controlling serotonin levels TPH2 influences many physiological processes including sleep cycles appetite and emotional status. Its activity is important for maintaining normal levels of serotonin in the synaptic cleft therefore contributing to overall neural communication.

Activity summary

The TPH2 protein also known as Tryptophan Hydroxylase 2 acts as a catalyst in the rate-limiting step of serotonin biosynthesis. It resides mostly in the brain specifically within neurons of the central nervous system where it facilitates the conversion of tryptophan to 5-hydroxytryptophan. TPH2 has a molecular mass of approximately 55 kDa. TPH2 expression is mainly observed in the raphe nuclei a region involved in mood regulation and other neural functions.

Pathways

TPH2 is involved in the serotonin biosynthesis pathway where it regulates serotonin production. This pathway is vital for mood and emotional regulation. TPH2 also interacts with enzymes like AADC (Aromatic L-amino acid decarboxylase) which further converts 5-hydroxytryptophan into serotonin. These interactions ensure a balanced production of serotonin essential for proper neuronal signaling and stable mental states.

Associated diseases and disorders

TPH2 has connections with mental health conditions such as depression and anxiety disorders. Altered TPH2 function can lead to reduced serotonin levels contributing to the pathophysiology of these disorders. Studies show that TPH2 may interact with proteins like MAOA (Monoamine oxidase A) where mutations or dysregulation can exacerbate mood disorders. Understanding TPH2's role offers insights into therapeutic strategies targeting serotonin imbalances in mental health conditions.

Product promise

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1 product image

  • Western blot - Anti-TPH2 antibody (ab121013), expandable thumbnail

    Western blot - Anti-TPH2 antibody (ab121013)

    Primary incubation was 1 hour. Detected by chemiluminescence.

    All lanes: Western blot - Anti-TPH2 antibody (AB121013) at 0.5 µg/mL

    All lanes: HEK293 lysate in RIPA buffer at 35 µg

    Predicted band size: 56 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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