AB211528

Anti-TPH2 antibody [CL2990] - C-terminal

Name: Anti-TPH2 antibody [CL2990] - C-terminal (ab211528) | Abcam
Brand: Abcam Limited
SKU: AB211528
Price: 520 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal TPH2 antibody. C-terminal. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TPH2 aa 400 to C-terminus.

View Alternative Names

NTPH, TPH2, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemical analysis of Paraffin-embedded human dorsal raphe tissue labeling TPH2 with ab211528 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemical analysis of Paraffin-embedded human cerebral cortex tissue labeling TPH2 with ab211528 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemical analysis of Paraffin-embedded rat dorsal raphe tissue labeling TPH2 with ab211528 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemical analysis of Paraffin-embedded mouse basal forebrain tissue labeling TPH2 with ab211528 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemical analysis of Paraffin-embedded rat cerebral cortex tissue labeling TPH2 with ab211528 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemical analysis of Paraffin-embedded mouse cerebral cortex tissue labeling TPH2 with ab211528 at 1/1000 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2990

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TPH2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IWU9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TPH2 protein also known as Tryptophan Hydroxylase 2 acts as a catalyst in the rate-limiting step of serotonin biosynthesis. It resides mostly in the brain specifically within neurons of the central nervous system where it facilitates the conversion of tryptophan to 5-hydroxytryptophan. TPH2 has a molecular mass of approximately 55 kDa. TPH2 expression is mainly observed in the raphe nuclei a region involved in mood regulation and other neural functions.

Biological function summary

TPH2 is essential for serotonin production impacting mood cognition and neurodevelopment. It functions independently rather than as part of a complex. By controlling serotonin levels TPH2 influences many physiological processes including sleep cycles appetite and emotional status. Its activity is important for maintaining normal levels of serotonin in the synaptic cleft therefore contributing to overall neural communication.

Pathways

TPH2 is involved in the serotonin biosynthesis pathway where it regulates serotonin production. This pathway is vital for mood and emotional regulation. TPH2 also interacts with enzymes like AADC (Aromatic L-amino acid decarboxylase) which further converts 5-hydroxytryptophan into serotonin. These interactions ensure a balanced production of serotonin essential for proper neuronal signaling and stable mental states.

TPH2 has connections with mental health conditions such as depression and anxiety disorders. Altered TPH2 function can lead to reduced serotonin levels contributing to the pathophysiology of these disorders. Studies show that TPH2 may interact with proteins like MAOA (Monoamine oxidase A) where mutations or dysregulation can exacerbate mood disorders. Understanding TPH2's role offers insights into therapeutic strategies targeting serotonin imbalances in mental health conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information TPH2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1581860 PubMed40406250

2025

Integrated analysis identifies P4HA2 as a key regulator of STAT1-mediated colorectal cancer progression and a potential biomarker for precision therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Qianshi Zhang,Yinan Zhang,Zhiwei Sun,Huanle Wang,Guohang Dai,Yue Meng,Shasha Shi,Shuangyi Ren

CNS neuroscience & therapeutics 30:e14922 PubMed39138640

2024

Myelin modulates the process of isoflurane anesthesia through the regulation of neural activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Wang,Rulan Yi,Xiaoling Liang,Ning Zhang,Fuwang Zhong,Yali Lu,Wenjia Chen,Tian Yu,Linyong Zhang,Haiying Wang,Liang Zhou

Communications biology 6:1155 PubMed37957344

2023

SMN deficiency perturbs monoamine neurotransmitter metabolism in spinal muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Valsecchi,Francesco Errico,Valentina Bassareo,Carmen Marino,Tommaso Nuzzo,Paola Brancaccio,Giusy Laudati,Antonella Casamassa,Manuela Grimaldi,Adele D'Amico,Manolo Carta,Enrico Bertini,Giuseppe Pignataro,Anna Maria D'Ursi,Alessandro Usiello

iScience 26:106707 PubMed37250336

2023

Oxytocin excites dorsal raphe serotonin neurons and bidirectionally gates their glutamate synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Saida Oubraim,Roh-Yu Shen,Samir Haj-Dahmane

eNeuro 9: PubMed36265904

2022

Distribution Features and Potential Effects of Serotonin in the Cerebrum of SOD1 G93A Transgenic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pei He,Binjun He,Shu Li,Wen Chai,Wei Rao,Yu Zhu,Wenzhi Chen,Ping Zhang,Xiong Zhang,Haili Pan,Renshi Xu

Journal of neuroinflammation 19:153 PubMed35706025

2022

Sciatic nerve stimulation alleviates acute neuropathic pain via modulation of neuroinflammation and descending pain inhibition in a rodent model.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-En Wong,Chia-Ying Hu,Po-Hsuan Lee,Chi-Chen Huang,Han-Wei Huang,Chih-Yuan Huang,Hsin-Tien Lo,Wentai Liu,Jung-Shun Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TPH2 antibody [CL2990] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [CL2990] - C-terminal (AB211528)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Secondary Antibodies

Primary Antibodies

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