Rabbit Recombinant Monoclonal TPH2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-frozen-sections-img77938.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286)")
![Immunoprecipitation - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunoprecipitation-img8457.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121763.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286)")
![Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-frozen-sections-img100368.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104774.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (AB240286)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested |
| Rat | Predicted | Expected | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Alternative names
Ntph, Tph2, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2
Recommended products
Rabbit Recombinant Monoclonal TPH2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19191
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240286 is the carrier-free version of Anti-TPH2 antibody [EPR19191] ab184505.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TPH2 protein also known as Tryptophan Hydroxylase 2 acts as a catalyst in the rate-limiting step of serotonin biosynthesis. It resides mostly in the brain specifically within neurons of the central nervous system where it facilitates the conversion of tryptophan to 5-hydroxytryptophan. TPH2 has a molecular mass of approximately 55 kDa. TPH2 expression is mainly observed in the raphe nuclei a region involved in mood regulation and other neural functions.
- Biological function summary
TPH2 is essential for serotonin production impacting mood cognition and neurodevelopment. It functions independently rather than as part of a complex. By controlling serotonin levels TPH2 influences many physiological processes including sleep cycles appetite and emotional status. Its activity is important for maintaining normal levels of serotonin in the synaptic cleft therefore contributing to overall neural communication.
- Pathways
TPH2 is involved in the serotonin biosynthesis pathway where it regulates serotonin production. This pathway is vital for mood and emotional regulation. TPH2 also interacts with enzymes like AADC (Aromatic L-amino acid decarboxylase) which further converts 5-hydroxytryptophan into serotonin. These interactions ensure a balanced production of serotonin essential for proper neuronal signaling and stable mental states.
- Associated diseases and disorders
TPH2 has connections with mental health conditions such as depression and anxiety disorders. Altered TPH2 function can lead to reduced serotonin levels contributing to the pathophysiology of these disorders. Studies show that TPH2 may interact with proteins like MAOA (Monoamine oxidase A) where mutations or dysregulation can exacerbate mood disorders. Understanding TPH2's role offers insights into therapeutic strategies targeting serotonin imbalances in mental health conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-frozen-sections-img77938.jpg "Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling TPH2 with Anti-TPH2 antibody [EPR19191] ab184505 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TPH2 antibody [EPR19191] ab184505).
![Immunoprecipitation - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunoprecipitation-img8457.jpg "Immunoprecipitation - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
Immunoprecipitation - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)
TPH2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with Anti-TPH2 antibody [EPR19191] ab184505 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-TPH2 antibody [EPR19191] ab184505 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate, 10μg (Input).
Lane 2: Anti-TPH2 antibody [EPR19191] ab184505 IP in Mouse brain lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TPH2 antibody [EPR19191] ab184505 in Mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TPH2 antibody [EPR19191] ab184505).
All lanes: Immunoprecipitation - Anti-TPH2 antibody [EPR19191] (Anti-TPH2 antibody [EPR19191] ab184505)
Predicted band size: 56 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121763.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)
Immunohistochemical analysis of paraffin-embedded Mouse brainstem tissue labeling TPH2 with Anti-TPH2 antibody [EPR19191] ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on dorsal raphe cells of mouse brainstem [PMID: 22912815]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TPH2 antibody [EPR19191] ab184505).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-frozen-sections-img100368.jpg "Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brainstem tissue labeling TPH2 with Anti-TPH2 antibody [EPR19191] ab184505 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on dorsal raphe cells of mouse brainstem [PMID: 22912815]. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TPH2 antibody [EPR19191] ab184505).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104774.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)
Immunohistochemical analysis of paraffin-embedded Rat brainstem tissue labeling TPH2 with Anti-TPH2 antibody [EPR19191] ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on dorsal raphe cells of rat brainstem [PMID: 22912815]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TPH2 antibody [EPR19191] ab184505).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107134.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TPH2 with Anti-TPH2 antibody [EPR19191] ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. No staining on mouse spleen. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TPH2 antibody [EPR19191] ab184505).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img50382.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling TPH2 with Anti-TPH2 antibody [EPR19191] ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. No staining on rat stomach. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TPH2 antibody [EPR19191] ab184505).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107134.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286), expandable thumbnail](https://content.abcam.com/products/images/tph2-antibody-epr19191-bsa-and-azide-free-ab240286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img50382.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] - BSA and Azide free (ab240286)")
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