Anti-TPH2 antibody [EPR25100-29] (ab288067)

Rabbit Recombinant Monoclonal TPH2 antibody. Suitable for mIHC, ICC/IF, IP, Flow Cyt, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human, Transfected cell line, Transfected cell lysate samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Anti-TPH2 antibody [EPR25100-29] (AB288067), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	ICC/IF	IP	Flow Cyt	WB	IHC-P	IHC-Fr
Human	Expected	Expected	Expected	Expected	Tested	Expected	Expected
Mouse	Tested	Expected	Tested	Expected	Tested	Tested	Tested
Rat	Expected	Expected	Expected	Expected	Tested	Tested	Expected
Transfected cell line	Not recommended	Tested	Not recommended	Tested	Not recommended	Tested	Not recommended
Transfected cell lysate	Not recommended	Not recommended	Tested	Not recommended	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line, Transfected cell lysate	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate	Dilution info 1/30	Notes -
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line	Dilution info 1/2000	Notes -
Species Mouse	Dilution info 1/2000	Notes -
Species Rat	Dilution info 1/2000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line, Transfected cell lysate	Dilution info -	Notes -

Target data

See full target information TPH2

Alternative names

NTPH, TPH2, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25100-29
Purification technique: Affinity purification Protein A
Specificity: No suitable positive material of human species is available for in-house IHC-P QC, and it worked well on overexpressed cells pellets of human species.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The TPH2 protein also known as Tryptophan Hydroxylase 2 acts as a catalyst in the rate-limiting step of serotonin biosynthesis. It resides mostly in the brain specifically within neurons of the central nervous system where it facilitates the conversion of tryptophan to 5-hydroxytryptophan. TPH2 has a molecular mass of approximately 55 kDa. TPH2 expression is mainly observed in the raphe nuclei a region involved in mood regulation and other neural functions.

Biological function summary

TPH2 is essential for serotonin production impacting mood cognition and neurodevelopment. It functions independently rather than as part of a complex. By controlling serotonin levels TPH2 influences many physiological processes including sleep cycles appetite and emotional status. Its activity is important for maintaining normal levels of serotonin in the synaptic cleft therefore contributing to overall neural communication.

Pathways

TPH2 is involved in the serotonin biosynthesis pathway where it regulates serotonin production. This pathway is vital for mood and emotional regulation. TPH2 also interacts with enzymes like AADC (Aromatic L-amino acid decarboxylase) which further converts 5-hydroxytryptophan into serotonin. These interactions ensure a balanced production of serotonin essential for proper neuronal signaling and stable mental states.

Associated diseases and disorders

TPH2 has connections with mental health conditions such as depression and anxiety disorders. Altered TPH2 function can lead to reduced serotonin levels contributing to the pathophysiology of these disorders. Studies show that TPH2 may interact with proteins like MAOA (Monoamine oxidase A) where mutations or dysregulation can exacerbate mood disorders. Understanding TPH2's role offers insights into therapeutic strategies targeting serotonin imbalances in mental health conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

Flow Cytometry - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human Tryptophan 5-hydroxylase 2 expression vector containing a myc-His tag cells labelling TPH2 with ab288067 at 1/500 dilution (0.1ug)( Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left) isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-TPH2 antibody [EPR25100-29] (ab288067)
TPH2 was immunoprecipitated from 0.35 mg Mouse midbrain tissue lysate 10 ug with ab288067 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288067 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse midbrain tissue lysate 10 ug
Lane 2: ab288067 IP in Mouse midbrain tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab288067 in mouse midbrain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
All lanes: Immunoprecipitation - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of paraffin-embedded Rat midbrain tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Positive staining in rat midbrain.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat midbrain (fresh) tissue labeling TPH2 with ab288067 at 1/500 (1.26 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on rat midbrain is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Western blot - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
All lanes: Western blot - Anti-TPH2 antibody [EPR25100-29] (ab288067) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containi a myc-His-tag®, whole cell lysate at 10 µg
Lane 2: HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containi a myc-His-tag®, whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T+OE-1169 cells labelling TPH2 with ab288067 at 1/500 (1.26 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody ready to use(Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged TPH2 expression vector. is observed. Myc-Tag Mouse mAb (Alexa Fluor^® 647) was used to counterstain tubulin at 1/100 (0.38ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) is ready to use.
Immunoprecipitation - Anti-TPH2 antibody [EPR25100-29] (ab288067)
TPH2 was immunoprecipitated from 0.35 mg HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containing a myc-His-tag® whole cell lysate 10 ug with ab288067 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288067 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containing a myc-His-tag® whole cell lysate 10 ug
Lane 2: ab288067 IP in HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containing a myc-His-tag® whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab288067 in HEK-293T transfected with Tryptophan 5-hydroxylase 2 expression vector containing a myc-His-tag® whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
All lanes: Immunoprecipitation - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Predicted band size: 56 kDa
Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse midbrain (fresh) tissue labeling TPH2 with ab288067 at 1/500 (1.26 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on mouse midbrain is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . No staining in rat kidney.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: kidney (PMID: 12511643).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
All lanes: Western blot - Anti-TPH2 antibody [EPR25100-29] (ab288067) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . No staining in mouse kidney.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Positive staining in neural fibers of rat cerebrum.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Positive staining in neural fibers of mouse cerebrum.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Cytoplasmic staining on (A) HEK-293T cells transfected with a TPH2 expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: kidney, spleen (PMID: 12511643).
Exposure time: Lanes 1-3: 70 seconds; Lanes 4-6: 26 seconds
All lanes: Western blot - Anti-TPH2 antibody [EPR25100-29] (ab288067) at 1/1000 dilution
Lane 1: Mouse midbrain tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Rat midbrain tissue lysate at 20 µg
Lane 5: Rat kidney tissue lysate at 20 µg
Lane 6: Rat spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR25100-29] (ab288067)
Immunohistochemical analysis of paraffin-embedded Mouse midbrain tissue labelling TPH2 with ab288067 at 1/2000 (0.315 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Positive staining in mouse midbrain.The section was incubated with ab288067 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-TPH2 antibody [EPR25100-29] (ab288067)
TPH2 Multiplex immunohistochemistry staining of Mouse midbrain tissue using rabbit Anti-TPH2 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse midbrain tissue staining THP2 with ab288067 at a 1/2000 (0.315 ug/ml) dilution, P2Y12 with Anti-P2Y12 antibody [EPR26298-93] ab300140 at 1/40000 (0.013 ug/ml) dilution and GFAP with Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at 1/1000 ( 1.325 ug/ml) dilution.
Panel A: merged staining of anti-THP2 (green; Opal™520), anti-P2Y12 (grey; Opal™570) and anti-GFAP (magenta; Opal™690) on mouse midbrain.
Panel B: anti-THP2 staining the serotonergic neurons in mouse midbrain.
Panel C: anti-P2Y12 staining microglia in mouse midbrain.
Panel D: anti-GFAP staining astrocytes in mouse midbrain.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab2888067, Anti-P2Y12 antibody [EPR26298-93] ab300140 and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.