Rabbit Polyclonal Tpit antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBX19 aa 1-300.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TBX19 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link O60806
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
| Dog | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Transcriptional regulator involved in developmental processes. Can activate POMC gene expression and repress the alpha glycoprotein subunit and thyroid-stimulating hormone beta promoters.
Alternative names
TPIT, TBX19, T-box transcription factor TBX19, T-box protein 19
Recommended products
Rabbit Polyclonal Tpit antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBX19 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TBX19 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link O60806
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tpit also known as TPIT or Tbx19 is a transcription factor that plays a significant role in the differentiation of certain pituitary cells. It has a molecular mass of approximately 62 kDa. Tpit is expressed in the pituitary gland specifically in the cells that produce pro-opiomelanocortin (POMC) which develop into corticotrophs and melanotrophs. Its expression is important for the proper functioning of these endocrine cells directly impacting the hormone production process.
- Biological function summary
Tpit acts as a regulator in pituitary development and functioning. It binds to DNA and activates the transcription of POMC leading to the production of hormones such as ACTH (adrenocorticotropic hormone) and MSH (melanocyte-stimulating hormone). Tpit is not a part of a larger complex but functions independently to exert its transcriptional regulation role. Through this mechanism Tpit influences metabolic and stress-related processes in the body.
- Pathways
Tpit is deeply involved in the hypothalamic-pituitary-adrenal (HPA) axis a critical stress response system that regulates cortisol production. Tpit regulates POMC expression which in turn affects downstream hormones like ACTH integral to the HPA axis. Moreover its activity is related to other proteins such as CRH (corticotropin-releasing hormone receptor) as they jointly control hormonal signaling pathways. Tpit ensures that the pituitary gland properly synthesizes and secretes hormones in response to various physiological stimuli.
- Associated diseases and disorders
Tpit mutations are associated with disorders such as isolated ACTH deficiency. This disorder results from the failure to produce ACTH leading to adrenal insufficiency and symptoms of fatigue and hypoglycemia. Tpit's role links it to other proteins such as NR5A1 (nuclear receptor subfamily 5 group A member 1) that influence pituitary function and development. Understanding Tpit's involvement in these disorders can help in the development of targeted therapies and improved diagnostic methods in endocrine diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Tpit antibody (ab155554)
10% SDS PAGE
All lanes: Western blot - Anti-Tpit antibody (ab155554) at 1/5000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 48 kDa
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