Anti-Tpit antibody [CL6251] ab243028 is a mouse monoclonal antibody that is used in Tpit IHC. Suitable for human samples.
- Antibody clone CL6251 is the most widely used clone for Tpit on the market
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028), expandable thumbnail](https://content.abcam.com/products/images/tpit-antibody-cl6251-ab243028--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67818.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028), expandable thumbnail](https://content.abcam.com/products/images/tpit-antibody-cl6251-ab243028--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88362.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)")
Publications
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- Pituitary 26:227-2362023TGFBR3L is associated with gonadotropin production in non-functioning gonadotroph pituitary neuroendocrine tumours.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnders Jensen Kolnes,Kristin Astrid Berland Øystese,Evelina Sjöstedt,Nicoleta Cristina Olarescu,Ansgar Heck,Jens Pahnke,Daniel Dahlberg,Jon Berg-Johnsen,Geir Ringstad,Olivera Casar-Borota,Jens Bollerslev,Anders Palmstrøm JørgensenPubMed 36952069
- Cells 11:2022CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Dong,Wenjian Shi,Yongliang Liu,Jingwu Li,Yu Zhang,Guilan Dong,Xiaoliu Dong,Hua GaoPubMed 35954244
- Acta histochemica et cytochemica 54:87-962021Metabolic Reprogramming Drives Pituitary Tumor Growth through Epigenetic Regulation of TERT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiromi Onizuka,Kenta Masui,Kosaku Amano,Takakazu Kawamata,Tomoko Yamamoto,Yoji Nagashima,Noriyuki ShibataPubMed 34276102
- Genes 11:2020Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from - and -Cell Lineages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeiko Taniguchi-Ponciano,Eduardo Peña-Martínez,Gloria Silva-Román,Sandra Vela-Patiño,Ana Laura Guzman-Ortiz,Hector Quezada,Erick Gomez-Apo,Laura Chavez-Macias,Sophia Mercado-Medrez,Guadalupe Vargas-Ortega,Ana Laura Espinosa-de-Los-Monteros,Baldomero Gonzales-Virla,Aldo Ferreira-Hermosillo,Etual Espinosa-Cardenas,Claudia Ramirez-Renteria,Ernesto Sosa,Blas Lopez-Felix,Gerardo Guinto,Daniel Marrero-Rodríguez,Moises MercadoPubMed 33261069
- Scientific reports 10:193732020Transcriptome and methylome analysis reveals three cellular origins of pituitary tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeiko Taniguchi-Ponciano,Sergio Andonegui-Elguera,Eduardo Peña-Martínez,Gloria Silva-Román,Sandra Vela-Patiño,Erick Gomez-Apo,Laura Chavez-Macias,Guadalupe Vargas-Ortega,Laura Espinosa-de-Los-Monteros,Baldomero Gonzalez-Virla,Carolina Perez,Aldo Ferreira-Hermosillo,Etual Espinosa-Cardenas,Claudia Ramirez-Renteria,Ernesto Sosa,Blas Lopez-Felix,Gerardo Guinto,Daniel Marrero-Rodríguez,Moises MercadoPubMed 33168897
- Cancers 11:2019How Valuable Is the RT-qPCR of Pituitary-Specific Transcription Factors for Identifying Pituitary Neuroendocrine Tumor Subtypes According to the New WHO 2017 Criteria?Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Eugenia Torregrosa-Quesada et. al.PubMed 31835737
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human TBX19 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60806
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Transcriptional regulator involved in developmental processes. Can activate POMC gene expression and repress the alpha glycoprotein subunit and thyroid-stimulating hormone beta promoters.
Alternative names
TPIT, TBX19, T-box transcription factor TBX19, T-box protein 19
Recommended products
Anti-Tpit antibody [CL6251] ab243028 is a mouse monoclonal antibody that is used in Tpit IHC. Suitable for human samples.
- Antibody clone CL6251 is the most widely used clone for Tpit on the market
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human TBX19 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60806
- Clone number
- CL6251
- Purification technique
- Affinity purification Protein A
- Epitope
- Binds to an epitope located within the peptide sequence VLGEPSLTSIAVSTW as determined by overlapping synthetic peptides
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tpit also known as TPIT or Tbx19 is a transcription factor that plays a significant role in the differentiation of certain pituitary cells. It has a molecular mass of approximately 62 kDa. Tpit is expressed in the pituitary gland specifically in the cells that produce pro-opiomelanocortin (POMC) which develop into corticotrophs and melanotrophs. Its expression is important for the proper functioning of these endocrine cells directly impacting the hormone production process.
- Biological function summary
Tpit acts as a regulator in pituitary development and functioning. It binds to DNA and activates the transcription of POMC leading to the production of hormones such as ACTH (adrenocorticotropic hormone) and MSH (melanocyte-stimulating hormone). Tpit is not a part of a larger complex but functions independently to exert its transcriptional regulation role. Through this mechanism Tpit influences metabolic and stress-related processes in the body.
- Pathways
Tpit is deeply involved in the hypothalamic-pituitary-adrenal (HPA) axis a critical stress response system that regulates cortisol production. Tpit regulates POMC expression which in turn affects downstream hormones like ACTH integral to the HPA axis. Moreover its activity is related to other proteins such as CRH (corticotropin-releasing hormone receptor) as they jointly control hormonal signaling pathways. Tpit ensures that the pituitary gland properly synthesizes and secretes hormones in response to various physiological stimuli.
- Associated diseases and disorders
Tpit mutations are associated with disorders such as isolated ACTH deficiency. This disorder results from the failure to produce ACTH leading to adrenal insufficiency and symptoms of fatigue and hypoglycemia. Tpit's role links it to other proteins such as NR5A1 (nuclear receptor subfamily 5 group A member 1) that influence pituitary function and development. Understanding Tpit's involvement in these disorders can help in the development of targeted therapies and improved diagnostic methods in endocrine diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028), expandable thumbnail](https://content.abcam.com/products/images/tpit-antibody-cl6251-ab243028--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67818.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)
Formalin-fixed, paraffin-embedded human pituitary gland tissue stained for Tpit with ab243028 at a 1:1000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028), expandable thumbnail](https://content.abcam.com/products/images/tpit-antibody-cl6251-ab243028--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88362.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (ab243028)
Negative control - Formalin-fixed, paraffin-embedded human tonsil tissue stained for Tpit with ab243028 at a 1:1000 dilution in immunohistochemical analysis.
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