AB243028

Anti-Tpit antibody [CL6251]

Name: Anti-Tpit antibody [CL6251] (ab243028) | Abcam
Brand: Abcam Limited
SKU: AB243028

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(7 Publications)

Anti-Tpit antibody [CL6251] (ab243028) is a mouse monoclonal antibody detecting Tpit in IHC-P. Suitable for Human.

View Alternative Names

TPIT, TBX19, T-box transcription factor TBX19, T-box protein 19

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

Formalin-fixed, paraffin-embedded human pituitary gland tissue stained for Tpit with ab243028 at a 1 : 1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

Negative control - Formalin-fixed, paraffin-embedded human tonsil tissue stained for Tpit with ab243028 at a 1 : 1000 dilution in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL6251

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TBX19 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60806

Epitope

Binds to an epitope located within the peptide sequence VLGEPSLTSIAVSTW as determined by overlapping synthetic peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-Tpit antibody [CL6251] (ab243028) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tpit also known as TPIT or Tbx19 is a transcription factor that plays a significant role in the differentiation of certain pituitary cells. It has a molecular mass of approximately 62 kDa. Tpit is expressed in the pituitary gland specifically in the cells that produce pro-opiomelanocortin (POMC) which develop into corticotrophs and melanotrophs. Its expression is important for the proper functioning of these endocrine cells directly impacting the hormone production process.

Biological function summary

Tpit acts as a regulator in pituitary development and functioning. It binds to DNA and activates the transcription of POMC leading to the production of hormones such as ACTH (adrenocorticotropic hormone) and MSH (melanocyte-stimulating hormone). Tpit is not a part of a larger complex but functions independently to exert its transcriptional regulation role. Through this mechanism Tpit influences metabolic and stress-related processes in the body.

Pathways

Tpit is deeply involved in the hypothalamic-pituitary-adrenal (HPA) axis a critical stress response system that regulates cortisol production. Tpit regulates POMC expression which in turn affects downstream hormones like ACTH integral to the HPA axis. Moreover its activity is related to other proteins such as CRH (corticotropin-releasing hormone receptor) as they jointly control hormonal signaling pathways. Tpit ensures that the pituitary gland properly synthesizes and secretes hormones in response to various physiological stimuli.

Tpit mutations are associated with disorders such as isolated ACTH deficiency. This disorder results from the failure to produce ACTH leading to adrenal insufficiency and symptoms of fatigue and hypoglycemia. Tpit's role links it to other proteins such as NR5A1 (nuclear receptor subfamily 5 group A member 1) that influence pituitary function and development. Understanding Tpit's involvement in these disorders can help in the development of targeted therapies and improved diagnostic methods in endocrine diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator involved in developmental processes. Can activate POMC gene expression and repress the alpha glycoprotein subunit and thyroid-stimulating hormone beta promoters.

See full target information TBX19

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Pituitary 26:227-236 PubMed36952069

2023

TGFBR3L is associated with gonadotropin production in non-functioning gonadotroph pituitary neuroendocrine tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Anders Jensen Kolnes,Kristin Astrid Berland Øystese,Evelina Sjöstedt,Nicoleta Cristina Olarescu,Ansgar Heck,Jens Pahnke,Daniel Dahlberg,Jon Berg-Johnsen,Geir Ringstad,Olivera Casar-Borota,Jens Bollerslev,Anders Palmstrøm Jørgensen

Cells 11: PubMed35954244

2022

CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Dong,Wenjian Shi,Yongliang Liu,Jingwu Li,Yu Zhang,Guilan Dong,Xiaoliu Dong,Hua Gao

Acta histochemica et cytochemica 54:87-96 PubMed34276102

2021

Metabolic Reprogramming Drives Pituitary Tumor Growth through Epigenetic Regulation of TERT.

Applications

Unspecified application

Species

Unspecified reactive species

Hiromi Onizuka,Kenta Masui,Kosaku Amano,Takakazu Kawamata,Tomoko Yamamoto,Yoji Nagashima,Noriyuki Shibata

Aging 13:11281-11295 PubMed33820867

2021

Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip.

Applications

Unspecified application

Species

Unspecified reactive species

Renjie Xu,Fei Zhang,Junlan Lu,Kexin Wang,Peng Pan,Ye Sun,Yuxin Zhang

Genes 11: PubMed33261069

2020

Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from - and -Cell Lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Taniguchi-Ponciano,Eduardo Peña-Martínez,Gloria Silva-Román,Sandra Vela-Patiño,Ana Laura Guzman-Ortiz,Hector Quezada,Erick Gomez-Apo,Laura Chavez-Macias,Sophia Mercado-Medrez,Guadalupe Vargas-Ortega,Ana Laura Espinosa-de-Los-Monteros,Baldomero Gonzales-Virla,Aldo Ferreira-Hermosillo,Etual Espinosa-Cardenas,Claudia Ramirez-Renteria,Ernesto Sosa,Blas Lopez-Felix,Gerardo Guinto,Daniel Marrero-Rodríguez,Moises Mercado

Scientific reports 10:19373 PubMed33168897

2020

Transcriptome and methylome analysis reveals three cellular origins of pituitary tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Taniguchi-Ponciano,Sergio Andonegui-Elguera,Eduardo Peña-Martínez,Gloria Silva-Román,Sandra Vela-Patiño,Erick Gomez-Apo,Laura Chavez-Macias,Guadalupe Vargas-Ortega,Laura Espinosa-de-Los-Monteros,Baldomero Gonzalez-Virla,Carolina Perez,Aldo Ferreira-Hermosillo,Etual Espinosa-Cardenas,Claudia Ramirez-Renteria,Ernesto Sosa,Blas Lopez-Felix,Gerardo Guinto,Daniel Marrero-Rodríguez,Moises Mercado

Cancers 11: PubMed31835737

2019

How Valuable Is the RT-qPCR of Pituitary-Specific Transcription Factors for Identifying Pituitary Neuroendocrine Tumor Subtypes According to the New WHO 2017 Criteria?

Applications

Unspecified application

Species

Unspecified reactive species

María Eugenia Torregrosa-Quesada,Araceli García-Martínez,Sandra Silva-Ortega,Sebastián Martínez-López,Rosa Cámara,Carmen Fajardo,Cristina Lamas,Ignacio Aranda,Antonio Picó

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tpit antibody [CL6251]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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