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Rabbit Polyclonal TPK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TPK1.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human TPK1. Database link Q9H3S4
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Expected
Tested
Tested
Mouse
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

Alternative names

Recommended products

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Rabbit Polyclonal TPK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TPK1.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human TPK1. Database link Q9H3S4
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

TPK1 plays an important role in energy metabolism. Thiamine pyrophosphate the product of TPK1 activity serves as an essential cofactor in several enzyme complexes including pyruvate dehydrogenase complex and alpha-ketoglutarate dehydrogenase complex. These complexes are involved in key metabolic pathways such as glycolysis and the citric acid cycle enabling the cell to produce energy efficiently. Therefore TPK1 activity supports energy production and cellular respiration.

Activity summary

TPK1 also known as thiamin pyrophosphokinase 1 is an enzyme responsible for the conversion of thiamine (vitamin B1) to thiamine pyrophosphate (TPP). The enzyme facilitates the transfer of pyrophosphate from ATP to thiamine which is the active form needed for cellular metabolism. TPK1 has a molecular mass of approximately 24 kDa. This enzyme is expressed in various tissues including liver kidney and brain reflecting its significant metabolic role.

Pathways

TPK1 significantly impacts carbohydrate metabolism and the Krebs cycle. It is involved in the thiamine metabolism pathway which is central to processing carbohydrates and generating energy. Proteins like pyruvate dehydrogenase and transketolase work in conjunction with TPK1 by using the TPP produced. These interactions highlight TPK1's role in linking thiamine conversion and energy-related pathways.

Associated diseases and disorders

TPK1 mutations can lead to thiamine metabolism dysfunction syndrome type 5 (THMD5) and contribute to Leigh syndrome a severe neurological disorder. TPK1 dysfunction impacts brain metabolism due to its high energy demands. The disorder shares a connection with pyruvate dehydrogenase as both proteins require TPP as a cofactor. Mutations that affect TPK1 can impair these pathways leading to neurological and metabolic complications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-TPK1 antibody (ab230263), expandable thumbnail

    Western blot - Anti-TPK1 antibody (ab230263)

    All lanes: Western blot - Anti-TPK1 antibody (AB230263) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate.

    Lane 2: Mouse kidney tissue lysate.

    Secondary

    All lanes: Goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TPK1 antibody (ab230263), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TPK1 antibody (ab230263)

    PC-3 (human prostate adenocarcinoma cell line) cells stained for TPK1 (green) using ab230263 at 1/100 dilution in ICC/IF. Secondary used is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (ab230263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (ab230263)

    Paraffin-embedded human kidney tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (ab230263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (ab230263)

    Paraffin-embedded human heart tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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