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BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Polyclonal TPK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TPK1.
IgG
Rabbit
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Expected | Tested | Tested |
Mouse | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
Thiamin pyrophosphokinase 1, hTPK1, Placental protein 20, Thiamine pyrophosphokinase 1, PP20, TPK1
Rabbit Polyclonal TPK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TPK1.
Thiamin pyrophosphokinase 1, hTPK1, Placental protein 20, Thiamine pyrophosphokinase 1, PP20, TPK1
IgG
Rabbit
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
TPK1 plays an important role in energy metabolism. Thiamine pyrophosphate the product of TPK1 activity serves as an essential cofactor in several enzyme complexes including pyruvate dehydrogenase complex and alpha-ketoglutarate dehydrogenase complex. These complexes are involved in key metabolic pathways such as glycolysis and the citric acid cycle enabling the cell to produce energy efficiently. Therefore TPK1 activity supports energy production and cellular respiration.
TPK1 also known as thiamin pyrophosphokinase 1 is an enzyme responsible for the conversion of thiamine (vitamin B1) to thiamine pyrophosphate (TPP). The enzyme facilitates the transfer of pyrophosphate from ATP to thiamine which is the active form needed for cellular metabolism. TPK1 has a molecular mass of approximately 24 kDa. This enzyme is expressed in various tissues including liver kidney and brain reflecting its significant metabolic role.
TPK1 significantly impacts carbohydrate metabolism and the Krebs cycle. It is involved in the thiamine metabolism pathway which is central to processing carbohydrates and generating energy. Proteins like pyruvate dehydrogenase and transketolase work in conjunction with TPK1 by using the TPP produced. These interactions highlight TPK1's role in linking thiamine conversion and energy-related pathways.
TPK1 mutations can lead to thiamine metabolism dysfunction syndrome type 5 (THMD5) and contribute to Leigh syndrome a severe neurological disorder. TPK1 dysfunction impacts brain metabolism due to its high energy demands. The disorder shares a connection with pyruvate dehydrogenase as both proteins require TPP as a cofactor. Mutations that affect TPK1 can impair these pathways leading to neurological and metabolic complications.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-TPK1 antibody (AB230263) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate.
Lane 2: Mouse kidney tissue lysate.
All lanes: Goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 27 kDa
PC-3 (human prostate adenocarcinoma cell line) cells stained for TPK1 (green) using ab230263 at 1/100 dilution in ICC/IF. Secondary used is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human kidney tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human heart tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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