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AB230263

Anti-TPK1 antibody

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(2 Publications)

Rabbit Polyclonal TPK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TPK1.

View Alternative Names

Thiamine pyrophosphokinase 1, hTPK1, Placental protein 20, Thiamin pyrophosphokinase 1, PP20, TPK1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)

Paraffin-embedded human heart tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TPK1 antibody (AB230263)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TPK1 antibody (AB230263)

PC-3 (human prostate adenocarcinoma cell line) cells stained for TPK1 (green) using ab230263 at 1/100 dilution in ICC/IF. Secondary used is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)

Paraffin-embedded human kidney tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-TPK1 antibody (AB230263)
  • WB

Supplier Data

Western blot - Anti-TPK1 antibody (AB230263)

All lanes:

Western blot - Anti-TPK1 antibody (ab230263) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate.

Lane 2:

Mouse kidney tissue lysate.

Secondary

All lanes:

Goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TPK1.

Q9H3S4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPK1 also known as thiamin pyrophosphokinase 1 is an enzyme responsible for the conversion of thiamine (vitamin B1) to thiamine pyrophosphate (TPP). The enzyme facilitates the transfer of pyrophosphate from ATP to thiamine which is the active form needed for cellular metabolism. TPK1 has a molecular mass of approximately 24 kDa. This enzyme is expressed in various tissues including liver kidney and brain reflecting its significant metabolic role.
Biological function summary

TPK1 plays an important role in energy metabolism. Thiamine pyrophosphate the product of TPK1 activity serves as an essential cofactor in several enzyme complexes including pyruvate dehydrogenase complex and alpha-ketoglutarate dehydrogenase complex. These complexes are involved in key metabolic pathways such as glycolysis and the citric acid cycle enabling the cell to produce energy efficiently. Therefore TPK1 activity supports energy production and cellular respiration.

Pathways

TPK1 significantly impacts carbohydrate metabolism and the Krebs cycle. It is involved in the thiamine metabolism pathway which is central to processing carbohydrates and generating energy. Proteins like pyruvate dehydrogenase and transketolase work in conjunction with TPK1 by using the TPP produced. These interactions highlight TPK1's role in linking thiamine conversion and energy-related pathways.

TPK1 mutations can lead to thiamine metabolism dysfunction syndrome type 5 (THMD5) and contribute to Leigh syndrome a severe neurological disorder. TPK1 dysfunction impacts brain metabolism due to its high energy demands. The disorder shares a connection with pyruvate dehydrogenase as both proteins require TPP as a cofactor. Mutations that affect TPK1 can impair these pathways leading to neurological and metabolic complications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP) utilizing UTP and therefore links the biosynthesis of TPP to pyrimidines metabolism (PubMed : 38547260). By producing thiamine pyrophosphate, a cofactor of the mitochondrial pyruvate dehydrogenase indirectly regulates pyruvate oxidation and lipogenesis (PubMed : 38547260). Although it can also catalyze thiamine phosphorylation using ATP and CTP in vitro, it does so with significantly lower efficiency and without physiological relevance evidence (PubMed : 11342111, PubMed : 38547260).
See full target information TPK1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed41031878

2025

Targeting specific kinase substrates rescues increased colitis severity induced by the Crohn's disease-linked LRRK2-N2081D variant.

Applications

Unspecified application

Species

Unspecified reactive species

George R Heaton,Xingjian Li,Xianting Li,Xiaoting Zhou,Yuanxi Zhang,Duc Tung Vu,Marc Oeller,Ozge Karayel,Quyen Q Hoang,Meltem Ece Kars,Nitika Kamath,Minghui Wang,Leonid Tarassishin,Matthias Mann,Inga Peter,Zhenyu Yue

Neuroreport 35:1000-1009 PubMed39190417

2024

Low expression of thiamine pyrophosphokinase-1 contributes to brain susceptibility to thiamine deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfeng Xia,Ting Qian,Guoqiang Fei,Xiaoqin Cheng,Lei Zhao,Shaoming Sang,Chunjiu Zhong
View all publications
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