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AB155284

Anti-TPMT antibody [EPR10820(B)]

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(2 Publications)

Rabbit Recombinant Monoclonal TPMT antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Thiopurine S-methyltransferase, Thiopurine methyltransferase, TPMT

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155284 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TPMT with ab155284 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-TPMT antibody [EPR10820(B)] (AB155284)
  • WB

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Western blot - Anti-TPMT antibody [EPR10820(B)] (AB155284)

All lanes:

Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

TF1 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa

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Flow Cytometry (Intracellular) - Anti-TPMT antibody [EPR10820(B)] (AB155284)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TPMT antibody [EPR10820(B)] (AB155284)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling TPMT with ab155284 at 1/100 dilution (red) compared with a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPMT with ab155284 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)

Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155284 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (AB155284)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155284 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-TPMT antibody [EPR10820(B)] (AB155284)
  • WB

Lab

Western blot - Anti-TPMT antibody [EPR10820(B)] (AB155284)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : TPMT knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HepG2 whole cell lysate (20 μg)
Lane 4 : Jurkat whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab155284 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab155284 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. ab155284 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)

Predicted band size: 28 kDa

false

  • Carrier free

    Anti-TPMT antibody [EPR10820(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10820(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thiopurine S-methyltransferase often called TPMT plays a role as an enzyme that catalyzes the S-methylation of thiopurine compounds like 6-mercaptopurine and azathioprine. It exists as a monomer with a molecular mass of about 28 kDa. In humans TPMT expresses mainly in the liver and kidney. The activity of TPMT varies among individuals due to genetic polymorphisms.
Biological function summary

TPMT is important in the metabolism of therapeutic thiopurine drugs. These drugs often require precise dosing to achieve effectiveness without toxicity. TPMT does not form part of a larger complex; it functions independently to methylate thiopurine drugs. Genetic variations can lead to different TPMT activity levels affecting a person's response to these drugs significantly.

Pathways

TPMT is integral to the drug metabolism pathway specifically in the detoxification and elimination processes of thiopurine drugs. While TPMT predominantly acts on thiopurines thioredoxin reductase 1 (TXNRD1) may also have a role in modulating redox balance and affect the thiopurine metabolism indirectly through related cellular processes. Together they influence the cellular response to drugs like azathioprine.

Genetic variants in TPMT significantly contribute to thiopurine drug intolerance or toxicity particularly in conditions like inflammatory bowel disease and acute lymphoblastic leukemia where these drugs are used. Patients with low TPMT activity are at risk for severe myelosuppression a life-threatening bone marrow suppression. TPMT's relationship with the enzyme inosine monophosphate dehydrogenase (IMPDH) also illustrates its connection to nucleotide synthesis further linking TPMT to therapeutic outcomes in cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor (PubMed : 18484748, PubMed : 657528). TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.
See full target information TPMT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 13:1195063 PubMed37404723

2023

Peptidyl-prolyl cis/trans isomerase Pin1 interacts with hepatitis B virus core particle, but not with HBc protein, to promote HBV replication.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeonjoong Kwon,Jumi Kim,Chanho Song,Muhammad Azhar Sajjad,Jiseon Ha,Jaesung Jung,Sun Park,Ho-Joon Shin,Kyongmin Kim

Biochemistry and biophysics reports 29:101223 PubMed35146136

2022

Band 3/anion exchanger 1/solute carrier family 4 member 1 expression as determinant of cellular sensitivity to selenite exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Yasunori Fukumoto,Kemmu Matsuhashi,Yu-Ki Tanaka,Noriyuki Suzuki,Yasumitsu Ogra
View all publications
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