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AB166909

Anti-TPMT antibody [EPR10821(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal TPMT antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Thiopurine S-methyltransferase, Thiopurine methyltransferase, TPMT

3 Images
Flow Cytometry (Intracellular) - Anti-TPMT antibody [EPR10821(B)] (AB166909)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TPMT antibody [EPR10821(B)] (AB166909)

Intracellular flow cytometric analysis of permeabilized K562 cells using ab166909 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-TPMT antibody [EPR10821(B)] (AB166909)
  • WB

Lab

Western blot - Anti-TPMT antibody [EPR10821(B)] (AB166909)

Lanes 1 - 4 : Merged signal (red and green). Green - ab166909 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab166909 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. ab166909 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

TPMT knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

K562 whole cell lysate at 20 µg

Predicted band size: 28 kDa

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Western blot - Anti-TPMT antibody [EPR10821(B)] (AB166909)
  • WB

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Western blot - Anti-TPMT antibody [EPR10821(B)] (AB166909)

All lanes:

Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

TF-1 cell lysate at 10 µg

Predicted band size: 28 kDa

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  • Carrier free

    Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10821(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thiopurine S-methyltransferase often called TPMT plays a role as an enzyme that catalyzes the S-methylation of thiopurine compounds like 6-mercaptopurine and azathioprine. It exists as a monomer with a molecular mass of about 28 kDa. In humans TPMT expresses mainly in the liver and kidney. The activity of TPMT varies among individuals due to genetic polymorphisms.
Biological function summary

TPMT is important in the metabolism of therapeutic thiopurine drugs. These drugs often require precise dosing to achieve effectiveness without toxicity. TPMT does not form part of a larger complex; it functions independently to methylate thiopurine drugs. Genetic variations can lead to different TPMT activity levels affecting a person's response to these drugs significantly.

Pathways

TPMT is integral to the drug metabolism pathway specifically in the detoxification and elimination processes of thiopurine drugs. While TPMT predominantly acts on thiopurines thioredoxin reductase 1 (TXNRD1) may also have a role in modulating redox balance and affect the thiopurine metabolism indirectly through related cellular processes. Together they influence the cellular response to drugs like azathioprine.

Genetic variants in TPMT significantly contribute to thiopurine drug intolerance or toxicity particularly in conditions like inflammatory bowel disease and acute lymphoblastic leukemia where these drugs are used. Patients with low TPMT activity are at risk for severe myelosuppression a life-threatening bone marrow suppression. TPMT's relationship with the enzyme inosine monophosphate dehydrogenase (IMPDH) also illustrates its connection to nucleotide synthesis further linking TPMT to therapeutic outcomes in cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor (PubMed : 18484748, PubMed : 657528). TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.
See full target information TPMT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:379 PubMed38816421

2024

Biallelic variants in CSMD1 are implicated in a neurodevelopmental disorder with intellectual disability and variable cortical malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Werren,Emily R Peirent,Henna Jantti,Alba Guxholli,Kinshuk Raj Srivastava,Naama Orenstein,Vinodh Narayanan,Wojciech Wiszniewski,Mateusz Dawidziuk,Pawel Gawlinski,Muhammad Umair,Amjad Khan,Shahid Niaz Khan,David Geneviève,Daphné Lehalle,K L I van Gassen,Jacques C Giltay,Renske Oegema,Richard H van Jaarsveld,Rafiullah Rafiullah,Gudrun A Rappold,Rachel Rabin,John G Pappas,Marsha M Wheeler,Michael J Bamshad,Yao-Chang Tsan,Matthew B Johnson,Catherine E Keegan,Anshika Srivastava,Stephanie L Bielas
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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