Armenian hamster Recombinant Monoclonal TPN antibody. Suitable for WB and reacts with Mouse samples.
IgG
Armenian hamster
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | IP | |
---|---|---|---|
Human | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Tapa, Tapa, Tapbp, Tapasin, TPN, TPSN, TAP-associated protein, TAP-binding protein
Armenian hamster Recombinant Monoclonal TPN antibody. Suitable for WB and reacts with Mouse samples.
IgG
Armenian hamster
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
5D3
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
TPN also known as Thyroid Peroxidase is an enzyme critical for thyroid hormone synthesis. The protein has a molecular weight of approximately 105 kDa. It is mainly expressed in the thyroid gland where it facilitates iodine organification a critical step in the production of thyroid hormones. TPN is a membrane-bound glycoprotein localized in the apical membrane of thyrocytes allowing it to participate directly in hormone production.
The enzyme TPN operates at the interface of thyroglobulin and iodide catalyzing the iodination of tyrosine residues within thyroglobulin. This process facilitated by TPN is key to the synthesis of thyroxine (T4) and triiodothyronine (T3) the main thyroid hormones. TPN functions within a larger complex involving hydrogen peroxide and cofactor flavin adenine dinucleotide (FAD) which together drive efficient hormone biosynthesis. Therefore any disruption in TPN activity can affect the availability of thyroid hormones.
TPN’s activity properly integrates into the thyroid hormone synthesis pathway and iodine metabolism. This pathway oversees the production and regulation of critical hormones that influence metabolism growth and development. The pathway involves various proteins including thyroglobulin which serves as a substrate for TPN's enzymatic action. Another related protein is thyroid-stimulating hormone receptor (TSHR) which regulates TPN activity through iodine uptake and hormone production in thyrocytes.
TPN holds significant connections to conditions such as Hashimoto's thyroiditis and Graves' disease. In Hashimoto's thyroiditis the immune system mistakenly creates autoantibodies against TPN leading to reduced hormone synthesis and hypothyroidism. Similarly TPN is a major antigen in Graves' disease where stimulating antibodies result in overproduction of hormones contributing to hyperthyroidism. In both disorders TPN's interaction with thyroglobulin and the immune response highlights its critical role in thyroid gland dysfunction.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
All lanes: Western blot - Anti-TPN antibody [5D3] (ab307298) at 1/1000 dilution
All lanes: Mouse spleen lysate 20 μg
Observed band size: 48 kDa
Exposure time: 3.25s
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Upregulating the expression of TPN protein by IFN gamma treatment (PMID: 11169257).
Exposure time: 3.25 seconds.
All lanes: Western blot - Anti-TPN antibody [5D3] (ab307298) at 1/1000 dilution
Lane 1: Untreated B16-F10 (Mouse skin melanoma), whole cell lysate 20 μg
Lane 2: B16-F10 treated with /ml IFN gamma for 16 hours, whole cell lysate 20 μg
Observed band size: 48 kDa
Exposure time: 3.25s
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