Skip to main content

Armenian hamster Recombinant Monoclonal TPN antibody. Suitable for WB and reacts with Mouse samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-TPN antibody [5D3] (AB307298), expandable thumbnail
  • Western blot - Anti-TPN antibody [5D3] (AB307298), expandable thumbnail

Key facts

Isotype

IgG

Host species

Armenian hamster

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIP
Human
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Target data

Function

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

Alternative names

Recommended products

Armenian hamster Recombinant Monoclonal TPN antibody. Suitable for WB and reacts with Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

5D3

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TPN also known as Thyroid Peroxidase is an enzyme critical for thyroid hormone synthesis. The protein has a molecular weight of approximately 105 kDa. It is mainly expressed in the thyroid gland where it facilitates iodine organification a critical step in the production of thyroid hormones. TPN is a membrane-bound glycoprotein localized in the apical membrane of thyrocytes allowing it to participate directly in hormone production.

Biological function summary

The enzyme TPN operates at the interface of thyroglobulin and iodide catalyzing the iodination of tyrosine residues within thyroglobulin. This process facilitated by TPN is key to the synthesis of thyroxine (T4) and triiodothyronine (T3) the main thyroid hormones. TPN functions within a larger complex involving hydrogen peroxide and cofactor flavin adenine dinucleotide (FAD) which together drive efficient hormone biosynthesis. Therefore any disruption in TPN activity can affect the availability of thyroid hormones.

Pathways

TPN’s activity properly integrates into the thyroid hormone synthesis pathway and iodine metabolism. This pathway oversees the production and regulation of critical hormones that influence metabolism growth and development. The pathway involves various proteins including thyroglobulin which serves as a substrate for TPN's enzymatic action. Another related protein is thyroid-stimulating hormone receptor (TSHR) which regulates TPN activity through iodine uptake and hormone production in thyrocytes.

Associated diseases and disorders

TPN holds significant connections to conditions such as Hashimoto's thyroiditis and Graves' disease. In Hashimoto's thyroiditis the immune system mistakenly creates autoantibodies against TPN leading to reduced hormone synthesis and hypothyroidism. Similarly TPN is a major antigen in Graves' disease where stimulating antibodies result in overproduction of hormones contributing to hyperthyroidism. In both disorders TPN's interaction with thyroglobulin and the immune response highlights its critical role in thyroid gland dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-TPN antibody [5D3] (ab307298), expandable thumbnail

    Western blot - Anti-TPN antibody [5D3] (ab307298)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    All lanes: Western blot - Anti-TPN antibody [5D3] (ab307298) at 1/1000 dilution

    All lanes: Mouse spleen lysate 20 μg

    Observed band size: 48 kDa

    Exposure time: 3.25s

  • Western blot - Anti-TPN antibody [5D3] (ab307298), expandable thumbnail

    Western blot - Anti-TPN antibody [5D3] (ab307298)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Upregulating the expression of TPN protein by IFN gamma treatment (PMID: 11169257).

    Exposure time: 3.25 seconds.

    All lanes: Western blot - Anti-TPN antibody [5D3] (ab307298) at 1/1000 dilution

    Lane 1: Untreated B16-F10 (Mouse skin melanoma), whole cell lysate 20 μg

    Lane 2: B16-F10 treated with /ml IFN gamma for 16 hours, whole cell lysate 20 μg

    Observed band size: 48 kDa

    Exposure time: 3.25s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com