AB307299

Anti-TPN antibody [5D3] - BSA and Azide free

Recombinant
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Armenian hamster Recombinant Monoclonal TPN antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

View Alternative Names

Tapa, Tapbp, Tapasin, TPN, TPSN, TAP-associated protein, TAP-binding protein

2 Images

Supplier Data

Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (AB307299)

This data was developed using ab307298, the same antibody clone in a different buffer formulation. Blocking/diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 3.25 seconds.

All lanes:

Western blot - Anti-TPN antibody [5D3] (<a href='/en-us/products/primary-antibodies/tpn-antibody-5d3-ab307298'>ab307298</a>) at 1/1000 dilution

All lanes:

Mouse spleen lysate 20 μg

Observed band size: 48 kDa

false

Exposure time: 3.25s

Supplier Data

Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (AB307299)

This data was developed using ab307298, the same antibody clone in a different buffer formulation. Blocking/diluting buffer and concentration : 5% NFDM/TBST. Upregulating the expression of TPN protein by IFN gamma treatment (PMID : 11169257). Exposure time : 3.25 seconds.

All lanes:

Western blot - Anti-TPN antibody [5D3] (<a href='/en-us/products/primary-antibodies/tpn-antibody-5d3-ab307298'>ab307298</a>) at 1/1000 dilution

Lane 1:

Untreated B16-F10 (Mouse skin melanoma), whole cell lysate 20 μg

Lane 2:

B16-F10 treated with /ml IFN gamma for 16 hours, whole cell lysate 20 μg

Observed band size: 48 kDa

false

Exposure time: 3.25s

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

5D3

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

See full target information Tapbp

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TPN antibody [5D3] - BSA and Azide free

Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (AB307299)

Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (AB307299)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise