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Armenian hamster Recombinant Monoclonal TPN antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

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Images

Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (AB307299), expandable thumbnail
  • Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (AB307299), expandable thumbnail

Key facts

Isotype

IgG

Host species

Armenian hamster

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-P
Human
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

Alternative names

Recommended products

Armenian hamster Recombinant Monoclonal TPN antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

5D3

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TPN also known as Thyroid Peroxidase is an enzyme critical for thyroid hormone synthesis. The protein has a molecular weight of approximately 105 kDa. It is mainly expressed in the thyroid gland where it facilitates iodine organification a critical step in the production of thyroid hormones. TPN is a membrane-bound glycoprotein localized in the apical membrane of thyrocytes allowing it to participate directly in hormone production.

Biological function summary

The enzyme TPN operates at the interface of thyroglobulin and iodide catalyzing the iodination of tyrosine residues within thyroglobulin. This process facilitated by TPN is key to the synthesis of thyroxine (T4) and triiodothyronine (T3) the main thyroid hormones. TPN functions within a larger complex involving hydrogen peroxide and cofactor flavin adenine dinucleotide (FAD) which together drive efficient hormone biosynthesis. Therefore any disruption in TPN activity can affect the availability of thyroid hormones.

Pathways

TPN’s activity properly integrates into the thyroid hormone synthesis pathway and iodine metabolism. This pathway oversees the production and regulation of critical hormones that influence metabolism growth and development. The pathway involves various proteins including thyroglobulin which serves as a substrate for TPN's enzymatic action. Another related protein is thyroid-stimulating hormone receptor (TSHR) which regulates TPN activity through iodine uptake and hormone production in thyrocytes.

Associated diseases and disorders

TPN holds significant connections to conditions such as Hashimoto's thyroiditis and Graves' disease. In Hashimoto's thyroiditis the immune system mistakenly creates autoantibodies against TPN leading to reduced hormone synthesis and hypothyroidism. Similarly TPN is a major antigen in Graves' disease where stimulating antibodies result in overproduction of hormones contributing to hyperthyroidism. In both disorders TPN's interaction with thyroglobulin and the immune response highlights its critical role in thyroid gland dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (ab307299), expandable thumbnail

    Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (ab307299)

    This data was developed using Anti-TPN antibody [5D3] ab307298, the same antibody clone in a different buffer formulation.
    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    All lanes: Western blot - Anti-TPN antibody [5D3] (Anti-TPN antibody [5D3] ab307298) at 1/1000 dilution

    All lanes: Mouse spleen lysate 20 μg

    Observed band size: 48 kDa

    Exposure time: 3.25s

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

  • Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (ab307299), expandable thumbnail

    Western blot - Anti-TPN antibody [5D3] - BSA and Azide free (ab307299)

    This data was developed using Anti-TPN antibody [5D3] ab307298, the same antibody clone in a different buffer formulation.
    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Upregulating the expression of TPN protein by IFN gamma treatment (PMID: 11169257).

    Exposure time: 3.25 seconds.

    All lanes: Western blot - Anti-TPN antibody [5D3] (Anti-TPN antibody [5D3] ab307298) at 1/1000 dilution

    Lane 1: Untreated B16-F10 (Mouse skin melanoma), whole cell lysate 20 μg

    Lane 2: B16-F10 treated with /ml IFN gamma for 16 hours, whole cell lysate 20 μg

    Observed band size: 48 kDa

    Exposure time: 3.25s

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Upregulating the expression of TPN protein by IFN gamma treatment (PMID: 11169257).

    Exposure time: 3.25 seconds.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com