Anti-TPN antibody [7F6] - BSA and Azide free

• WB

Lab

Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (AB255872)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The double bands observed in lane 5 is consistent with what has been described in the literatures (PMID : 29107940, 17620360).

Exposure time : lane 1-3 : 6 seconds; lane 4-5 : 3 seconds.

This image was produced using the same antibody clone but in a different formulation ab252869, PBS, sodium azide, glycerol and BSA.

All lanes:

Western blot - Anti-TPN antibody [7F6] (<a href='/en-us/products/primary-antibodies/tpn-antibody-7f6-ab252869'>ab252869</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HUVEC (human umbilical vein endothelial cell), whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 4:

Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate at 20 µg

Lane 5:

Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 48 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (AB255872)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

This image was produced using the same antibody clone but in a different formulation ab252869, PBS, sodium azide, glycerol and BSA.

All lanes:

Western blot - Anti-TPN antibody [7F6] (<a href='/en-us/products/primary-antibodies/tpn-antibody-7f6-ab252869'>ab252869</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa treated with 100 U/ml IFN gamma for 24 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 48 kDa

Observed band size: 47 kDa

false