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Rabbit Recombinant Monoclonal TPN antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples.

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Images

Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (AB288574), expandable thumbnail
  • Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (AB288574), expandable thumbnail
  • Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (AB288574), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (AB288574), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (AB288574), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Not recommended
Tested
Tested
Tested
Tested
Mouse
Not recommended
Tested
Expected
Expected
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

This product is not suitable for IHC with samples from mouse.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

This product is not suitable for IHC with samples from mouse.

Species
Rat
Dilution info
-
Notes

This product is not suitable for IHC with samples from mouse.

Target data

Function

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

Alternative names

NGS17, TAPA, TAPBP, Tapasin, TPN, TPSN, NGS-17, TAP-associated protein, TAP-binding protein

Recommended products

Rabbit Recombinant Monoclonal TPN antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25083-20
Purification technique
Affinity purification Protein A
Specificity

This product is not suitable for IHC with samples from mouse.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TPN also known as Thyroid Peroxidase is an enzyme critical for thyroid hormone synthesis. The protein has a molecular weight of approximately 105 kDa. It is mainly expressed in the thyroid gland where it facilitates iodine organification a critical step in the production of thyroid hormones. TPN is a membrane-bound glycoprotein localized in the apical membrane of thyrocytes allowing it to participate directly in hormone production.

Biological function summary

The enzyme TPN operates at the interface of thyroglobulin and iodide catalyzing the iodination of tyrosine residues within thyroglobulin. This process facilitated by TPN is key to the synthesis of thyroxine (T4) and triiodothyronine (T3) the main thyroid hormones. TPN functions within a larger complex involving hydrogen peroxide and cofactor flavin adenine dinucleotide (FAD) which together drive efficient hormone biosynthesis. Therefore any disruption in TPN activity can affect the availability of thyroid hormones.

Pathways

TPN’s activity properly integrates into the thyroid hormone synthesis pathway and iodine metabolism. This pathway oversees the production and regulation of critical hormones that influence metabolism growth and development. The pathway involves various proteins including thyroglobulin which serves as a substrate for TPN's enzymatic action. Another related protein is thyroid-stimulating hormone receptor (TSHR) which regulates TPN activity through iodine uptake and hormone production in thyrocytes.

Associated diseases and disorders

TPN holds significant connections to conditions such as Hashimoto's thyroiditis and Graves' disease. In Hashimoto's thyroiditis the immune system mistakenly creates autoantibodies against TPN leading to reduced hormone synthesis and hypothyroidism. Similarly TPN is a major antigen in Graves' disease where stimulating antibodies result in overproduction of hormones contributing to hyperthyroidism. In both disorders TPN's interaction with thyroglobulin and the immune response highlights its critical role in thyroid gland dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Upregulating the expression of TPN protein by IFN gamma treatment (PMID: 11169257)

    All lanes: Western blot - Anti-TPN antibody [EPR25083-20] (Anti-TPN antibody [EPR25083-20] ab288565) at 1/1000 dilution

    Lane 1: Untreated HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

    Lane 2: HeLa treated with 100 ng/ml IFN gamma for 16 hours, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

    Exposure time: 48s

  • Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Upregulating the expression of TPN protein by IFN gamma treatment (PMID: 11169257)

    All lanes: Western blot - Anti-TPN antibody [EPR25083-20] (Anti-TPN antibody [EPR25083-20] ab288565) at 1/1000 dilution

    Lane 1: Untreated B16-F10 (Mouse skin melanoma), whole cell lysate at 10 µg

    Lane 2: B16-F10 treated with 100 ng/ml IFN gamma for 16 hours, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

    Exposure time: 48s

  • Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Western blot - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure times:

    Lanes 1 & 2: 48 seconds

    Lane 3: 3 minutes

    Lane 4: 26 seconds

    Laned 5 & 6: 15 seconds

    All lanes: Western blot - Anti-TPN antibody [EPR25083-20] (Anti-TPN antibody [EPR25083-20] ab288565) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Lane 3: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

    Lane 5: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TPN with Anti-TPN antibody [EPR25083-20] ab288565 at 1/500 (1.002 ug/ml) followed by ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Cytoplasmic staining on the stromal cells of human colon (PMID: 26310568). The section was incubated with Anti-TPN antibody [EPR25083-20] ab288565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling TPN with Anti-TPN antibody [EPR25083-20] ab288565 at 1/500 (1.002 ug/ml) followed by ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Mainly cytoplasmic staining on the leukocytes of human lung. The section was incubated with Anti-TPN antibody [EPR25083-20] ab288565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labelling TPN with Anti-TPN antibody [EPR25083-20] ab288565 at 1/500 (1.002 ug/ml) followed by ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Cytoplasmic staining on the cancer cells and stromal cells of human colon carcinoma (PMID: 26310568). The section was incubated with Anti-TPN antibody [EPR25083-20] ab288565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labelling TPN with Anti-TPN antibody [EPR25083-20] ab288565 at 1/500 (1.002 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Cytoplasmic staining on the cancer cells and stromal cells of human lung adenocarcinoma. The section was incubated with Anti-TPN antibody [EPR25083-20] ab288565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling TPN with Anti-TPN antibody [EPR25083-20] ab288565 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat cells labelling TPN with Anti-TPN antibody [EPR25083-20] ab288565 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Jurkat cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488), preadsorbed, at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TPN antibody [EPR25083-20] - BSA and Azide free (ab288574)

    This data was developed using Anti-TPN antibody [EPR25083-20] ab288565, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling TPN with Anti-TPN antibody [EPR25083-20] ab288565 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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