AB54685

Anti-TPP1 antibody [3B1]

Name: Anti-TPP1 antibody [3B1] (ab54685) | Abcam
Brand: Abcam Limited
SKU: AB54685
Price: 675 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(17 Publications)

Mouse Monoclonal TPP1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TPP1 aa 150-350.

View Alternative Names

CLN2, GIG1, UNQ267/PRO304, TPP1, Tripeptidyl-peptidase 1, TPP-1, Cell growth-inhibiting gene 1 protein, Lysosomal pepstatin-insensitive protease, Tripeptidyl aminopeptidase, Tripeptidyl-peptidase I, LPIC, TPP-I

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [3B1] (AB54685)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [3B1] (AB54685)

TPP1 antibody (ab54685) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-TPP1 antibody [3B1] (AB54685)

Flow Cyt

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Flow Cytometry - Anti-TPP1 antibody [3B1] (AB54685)

Overlay histogram showing JEG3 cells stained with ab54685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54685, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.

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Western blot - Anti-TPP1 antibody [3B1] (AB54685)

TPP1 antibody (ab54685) at 1ug/lane + A-431 cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-TPP1 antibody [3B1] (ab54685)

Predicted band size: 61 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3B1

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human TPP1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

O14773

Specificity

This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tripeptidyl peptidase I (TPP1) also known as CLN2 is a lysosomal serine protease with a molecular mass of about 61 kDa. TPP1 is expressed in numerous tissues with notable activity in the brain and retinal cells. Mechanically TPP1's primary function includes cleaving N-terminal tripeptides from substrates an important step in protein degradation and turnover within the lysosome.

Biological function summary

This protease plays an essential role in maintaining cellular function by preventing the accumulation of partially degraded proteins. TPP1 exists as part of a lysosomal enzyme complex involved in the catabolic process that recycles cellular components. Its action ensures proper protein homeostasis protecting cells from toxic buildup that can lead to cellular dysfunction.

Pathways

TPP1 is a part of the lysosomal degradation pathway critical for cellular waste management and nutrient recycling. This pathway intersects with broader metabolic routes where TPP1 activity supports the catabolism of intracytoplasmic substrates. TPP1 also interacts with proteins such as cathepsin D another enzyme contributing to proteolytic pathways to maintain the balance and efficiency of protein degradation processes.

TPP1 mutations are associated with neuronal ceroid lipofuscinosis type 2 (CLN2 disease) a rare neurodegenerative disorder. This disease involves progressive brain and retinal degeneration due to lysosomal storage malfunction. The TPP1 connection to CLN2 disease highlights its essential role in neurological health. Additionally TPP1 has links to Batten disease where compromised enzyme function leads to severe neurological symptoms. Other proteins like CLN3 may also interact with TPP1 in these disease contexts influencing their progression and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lysosomal serine protease with tripeptidyl-peptidase I activity (PubMed : 11054422, PubMed : 19038966, PubMed : 19038967). May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases (PubMed : 11054422, PubMed : 19038966, PubMed : 19038967). Requires substrates with an unsubstituted N-terminus (PubMed : 19038966).

See full target information TPP1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2022:6856768 PubMed36147632

2022

Tripeptidyl Peptidase 1 Regulates Human Trophoblast Cell Proliferation Implying a Role in Placentation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Evdokia Dimitriadis

Cell reports 40:111058 PubMed35830804

2022

Treadmill exercise reduces α-synuclein spreading via PPARα.

Applications

Unspecified application

Species

Unspecified reactive species

Debashis Dutta,Ramesh Kumar Paidi,Sumita Raha,Avik Roy,Sujyoti Chandra,Kalipada Pahan

Stem cell research 53:102323 PubMed33845243

2021

Generation of pathogenic TPP1 mutations in human stem cells as a model for neuronal ceroid lipofuscinosis type 2 disease.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Adriana M Prada,Michael Schmidt,Eric M Morrow

Nucleic acids research 49:3692-3708 PubMed33764477

2021

Effect of small molecule eRF3 degraders on premature termination codon readthrough.

Applications

Unspecified application

Species

Unspecified reactive species

Alireza Baradaran-Heravi,Aruna D Balgi,Sara Hosseini-Farahabadi,Kunho Choi,Cristina Has,Michel Roberge

Aging 13:6025-6040 PubMed33568575

2021

SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Chen,Zude Liu,Hantao Wang,Lie Qian,Zhanchun Li,Qingxin Song,Guibin Zhong

Reproductive biology and endocrinology : RB&E 18:124 PubMed33317560

2020

Tripeptidyl peptidase I promotes human endometrial epithelial cell adhesive capacity implying a role in receptivity.

Applications

Unspecified application

Species

Unspecified reactive species

Leilani L Santos,Cheuk Kwan Ling,Evdokia Dimitriadis

Science advances 6: PubMed33310843

2020

Exploiting the diphtheria toxin internalization receptor enhances delivery of proteins to lysosomes for enzyme replacement therapy.

Applications

Unspecified application

Species

Unspecified reactive species

S N Sugiman-Marangos,G L Beilhartz,X Zhao,D Zhou,R Hua,P K Kim,J M Rini,B A Minassian,R A Melnyk

PloS one 14:e0216423 PubMed31120902

2019

The antimalarial drug mefloquine enhances TP53 premature termination codon readthrough by aminoglycoside G418.

Applications

Unspecified application

Species

Unspecified reactive species

Michael W Ferguson,Chloe A N Gerak,Christalle C T Chow,Ettore J Rastelli,Kyle E Elmore,Florian Stahl,Sara Hosseini-Farahabadi,Alireza Baradaran-Heravi,Don M Coltart,Michel Roberge

Journal of immunology (Baltimore, Md. : 1950) 201:1875-1888 PubMed30135181

2018

Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Vigneron,Violette Ferrari,Benoît J Van den Eynde,Peter Cresswell,Ralf M Leonhardt

Orphanet journal of rare diseases 13:54 PubMed29631617

2018

Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses.

Applications

Species

Unspecified reactive species

Ni Sima,Rong Li,Wei Huang,Miao Xu,Jeanette Beers,Jizhong Zou,Steven Titus,Elizabeth A Ottinger,Juan J Marugan,Xing Xie,Wei Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TPP1 antibody [3B1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [3B1] (AB54685)

Flow Cytometry - Anti-TPP1 antibody [3B1] (AB54685)

Western blot - Anti-TPP1 antibody [3B1] (AB54685)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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