AB119987

Anti-TPPP antibody [EPR3315]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TPPP antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TPPP1, TPPP, Tubulin polymerization-promoting protein, 25 kDa brain-specific protein, TPPP/p25, p24, p25-alpha

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3315] (AB119987)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3315] (AB119987)

ab119987 at 1/100 dilution staining TPPP in Paraffin-embedded Human astrocytoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TPPP antibody [EPR3315] (AB119987)

The cell lysates were prepared in 1%SDS Hot lysis method

All lanes:

Western blot - Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa

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Lab

Western blot - Anti-TPPP antibody [EPR3315] (AB119987)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution

All lanes:

Human brain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3315

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98°C, below boiling, and then let cool for 10-20 min.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPPP also known as Tubulin Polymerization Promoting Protein or p25 is a small protein with an approximate mass of 25 kDa. TPPP is expressed mainly in the brain particularly abundant in oligodendrocytes. This protein plays an important role in regulating the polymerization of tubulin into microtubules. Through its binding to tubulin TPPP stabilizes microtubule structures facilitating proper microtubule assembly and maintenance. The modulation of these structures is essential for cellular operations like intracellular transport and cell division.

Biological function summary

The protein participates in the dynamics of cytoskeletal elements critical for maintaining cell shape and function. TPPP is part of cytoskeletal networks where it contributes to cellular integrity in neurons. TPPP's activity influences the formation and maintenance of myelin the insulating layer enveloping nerve fibers which is important for fast and efficient nerve impulse conduction. In its role TPPP interacts cooperatively with other cytoskeletal proteins enhancing microtubule resilience and cell structural stability.

Pathways

TPPP interacts in processes involving microtubule organization and stabilization. It is integral in the MAPK/ERK signaling pathway which is prominent in regulating cellular processes like proliferation and differentiation. Additionally TPPP may interplay with proteins such as MAP2 and Tau both involved in the MAP-kinase pathways regulating microtubule dynamics indicating a collaborative network in cellular signaling and structural reinforcement in neural cells.

TPPP has correlations with neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. Abnormal regulation or aggregation of TPPP is linked to the formation of pathological protein inclusions found in these conditions. The interaction between TPPP and Alpha-synuclein is particularly notable as TPPP may regulate or enhance the aggregation of Alpha-synuclein contributing to the development of Lewy bodies in Parkinson's. Understanding these interactions and pathways is essential for developing therapeutic strategies targeting these neurodegenerative disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed : 31522887). Acts as a microtubule nucleation factor in oligodendrocytes : specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (PubMed : 31522887, PubMed : 33831707). Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (PubMed : 31522887). Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (PubMed : 21316364, PubMed : 21995432). In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (PubMed : 17105200, PubMed : 17693641, PubMed : 18028908, PubMed : 26289831). Regulates microtubule dynamics by promoting tubulin acetylation : acts by inhibiting the tubulin deacetylase activity of HDAC6 (PubMed : 20308065, PubMed : 23093407). Also regulates cell migration : phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility (PubMed : 23093407). Plays a role in cell proliferation by regulating the G1/S-phase transition (PubMed : 23355470). Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (PubMed : 22328514).

See full target information TPPP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:38532 PubMed27922126

2016

Viral and bacterial co-infection in severe pneumonia triggers innate immune responses and specifically enhances IP-10: a translational study.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Hoffmann,Daniela Machado,Olivier Terrier,Stephane Pouzol,Mélina Messaoudi,Wilma Basualdo,Emilio E Espínola,Rosa M Guillen,Manuel Rosa-Calatrava,Valentina Picot,Thomas Bénet,Hubert Endtz,Graciela Russomando,Gláucia Paranhos-Baccalà

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TPPP antibody [EPR3315]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3315] (AB119987)

Western blot - Anti-TPPP antibody [EPR3315] (AB119987)

Western blot - Anti-TPPP antibody [EPR3315] (AB119987)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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