AB84516

Anti-TPR antibody

Name: Anti-TPR antibody (ab84516) | Abcam
Brand: Abcam Limited
SKU: AB84516
Price: 420 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(22 Publications)

Rabbit Polyclonal TPR antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human Nucleoprotein TPR aa 2300 to C-terminus.

View Alternative Names

Nucleoprotein TPR, Megator, NPC-associated intranuclear protein, Translocated promoter region protein, TPR

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPR antibody (AB84516)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPR antibody (AB84516)

ab84516 at a 1/250 dilution, staining in human TPR in human prostate carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPR antibody (AB84516)

ab84516 at a 1/250 dilution, staining TPR in human breast carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-TPR antibody (AB84516)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TPR antibody (AB84516)

ab84516 at a 1/250 dilution, staining human TPR in NBF-fixed asynchronous HeLa cells by immunocytochemistry. Detection : anti-rabbit IgG-FITC conjugate used at a dilution of 1/100.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPR antibody (AB84516)

ab84516 at a 1 : 250 dilution, staining mouse TPR in mouse squamous cell carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human Nucleoprotein TPR aa 2300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P12270

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "Epitope exposure is recommended. Exposure with citrate buffer will enhance staining. Likely to work with frozen sections.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "Permeabilization with Triton-X 100 is recommended." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "Epitope exposure is recommended. Exposure with citrate buffer will enhance staining. Likely to work with frozen sections.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Concentration is optimized for IHC and not determined

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified using an epitope specific to TPR immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The translocated promoter region protein (TPR) also known commonly as 'horse TPR' consists of approximately 267 kDa. TPR is a nucleoporin protein primarily expressed in the nuclear envelope of eukaryotic cells. It serves as a structural and regulatory component of the nuclear pore complex (NPC) playing a critical role in nucleocytoplasmic transport. A structural scaffold TPR facilitates the organization and entrance of nuclear transport receptors and cargo molecules through the NPC impacting the efficient movement of proteins and RNA across the nuclear membrane.

Biological function summary

TPR interacts with other nucleoporins within the NPC to ensure proper nuclear function. TPR is integral in maintaining the spatial organization and transport selectivity within the nucleoplasm-cytoplasm interface by forming an interaction network with other proteins like NUP98 and NUP153. TPR functions as part of the NPC assembly contributing to essential cellular processes like gene expression regulation and DNA repair by participating in chromatin organization.

Pathways

TPR integrates into the nuclear transport pathway and it also plays a role in the MAPK signaling pathway. Within the nuclear transport pathway TPR facilitates the proper translocation of molecules necessary for downstream cellular functions. In the MAPK signaling pathway it synchronizes with other proteins like ERK to control processes such as proliferation and differentiation. TPR's interaction with these pathways positions it as an important player in cellular communication and response.

TPR has connections to cancer and neurodegenerative diseases. In various cancers abnormal TPR function impacts cell growth and proliferation by altering nuclear transport dynamics. Additionally it associates with Alzheimer's disease through its interactions with tau protein affecting neuronal function. These connections make TPR a significant target for understanding disease mechanisms and developing therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. According to some authors, plays a limited role in the regulation of nuclear protein export (PubMed : 11952838, PubMed : 22253824). Also plays a role as a structural and functional element of the perinuclear chromatin distribution; involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases.

See full target information Nucleoprotein TPR

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39625470

2024

TPR is required for cytoplasmic chromatin fragment formation during senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Bethany M Bartlett,Yatendra Kumar,Shelagh Boyle,Tamoghna Chowdhury,Andrea Quintanilla,Charlene Boumendil,Juan Carlos Acosta,Wendy A Bickmore

Cells 12: PubMed38067185

2023

Tpr Misregulation in Hippocampal Neural Stem Cells in Mouse Models of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Subash C Malik,Jia-Di Lin,Stephanie Ziegler-Waldkirch,Stefan Tholen,Sachin S Deshpande,Marius Schwabenland,Oliver Schilling,Andreas Vlachos,Melanie Meyer-Luehmann,Christian Schachtrup

Journal of cell science 136: PubMed37305998

2023

Y-complex nucleoporins independently contribute to nuclear pore assembly and gene regulation in neuronal progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Clarisse Orniacki,Annalisa Verrico,Stéphane Pelletier,Benoit Souquet,Fanny Coulpier,Laurent Jourdren,Serena Benetti,Valérie Doye

Journal of experimental & clinical cancer research : CR 41:273 PubMed36096808

2022

Combined alteration of lamin and nuclear morphology influences the localization of the tumor-associated factor AKTIP.

Applications

Unspecified application

Species

Unspecified reactive species

Mattia La Torre,Chiara Merigliano,Klizia Maccaroni,Alexandre Chojnowski,Wah Ing Goh,Maria Giubettini,Fiammetta Vernì,Cristina Capanni,Daniela Rhodes,Graham Wright,Brian Burke,Silvia Soddu,Romina Burla,Isabella Saggio

Cells 10: PubMed34063931

2021

Nucleoporin TPR Affects C2C12 Myogenic Differentiation via Regulation of Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Uhlířová,Lenka Šebestová,Karel Fišer,Tomáš Sieger,Jindřiška Fišerová,Pavel Hozák

Cell 184:2860-2877.e22 PubMed33964210

2021

Parental genome unification is highly error-prone in mammalian embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Tommaso Cavazza,Yuko Takeda,Antonio Z Politi,Magomet Aushev,Patrick Aldag,Clara Baker,Meenakshi Choudhary,Jonas Bucevičius,Gražvydas Lukinavičius,Kay Elder,Martyn Blayney,Andrea Lucas-Hahn,Heiner Niemann,Mary Herbert,Melina Schuh

The Journal of cell biology 220: PubMed33570570

2021

Computational analyses reveal spatial relationships between nuclear pore complexes and specific lamins.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Kittisopikul,Takeshi Shimi,Meltem Tatli,Joseph Riley Tran,Yixian Zheng,Ohad Medalia,Khuloud Jaqaman,Stephen A Adam,Robert D Goldman

Genes & development 34:1619-1636 PubMed33122293

2020

Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Pinzaru,Mike Kareh,Noa Lamm,Eros Lazzerini-Denchi,Anthony J Cesare,Agnel Sfeir

Nature communications 11:4875 PubMed32978388

2020

Characterization of BRCA1-deficient premalignant tissues and cancers identifies Plekha5 as a tumor metastasis suppressor.

Applications

Unspecified application

Species

Unspecified reactive species

Jianlin Liu,Ragini Adhav,Kai Miao,Sek Man Su,Lihua Mo,Un In Chan,Xin Zhang,Jun Xu,Jianjie Li,Xiaodong Shu,Jianming Zeng,Xu Zhang,Xueying Lyu,Lakhansing Pardeshi,Kaeling Tan,Heng Sun,Koon Ho Wong,Chuxia Deng,Xiaoling Xu

The Journal of cell biology 218:2962-2981 PubMed31375530

2019

Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore.

Applications

Unspecified application

Species

Unspecified reactive species

Rakefet Ben-Yishay,Amir Mor,Amit Shraga,Asaf Ashkenazy-Titelman,Noa Kinor,Avital Schwed-Gross,Avi Jacob,Noga Kozer,Pramod Kumar,Yuval Garini,Yaron Shav-Tal

View all publications

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Product promise

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Anti-TPR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPR antibody (AB84516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPR antibody (AB84516)

Immunocytochemistry/ Immunofluorescence - Anti-TPR antibody (AB84516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPR antibody (AB84516)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Product promise