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AB249609

Anti-TPR antibody [EPR8982] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TPR antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Nucleoprotein TPR, Megator, NPC-associated intranuclear protein, Translocated promoter region protein, TPR

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TPR antibody [EPR8982] - BSA and Azide free (AB249609)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TPR antibody [EPR8982] - BSA and Azide free (AB249609)

This data was developed using ab170940, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling TPR with ab170940 at 1/100 dilution.

Western blot - Anti-TPR antibody [EPR8982] - BSA and Azide free (AB249609)
  • WB

Supplier Data

Western blot - Anti-TPR antibody [EPR8982] - BSA and Azide free (AB249609)

This data was developed using ab170940, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TPR antibody [EPR8982] (<a href='/en-us/products/primary-antibodies/tpr-antibody-epr8982-ab170940'>ab170940</a>) at 1/1000 dilution

Lane 1:

HeLa + Camptothecin cell lysates at 10 µg

Lanes 2 and 4:

HeLa cell lysates at 10 µg

Lane 3:

HeLa + Staurosporine cell lysates at 10 µg

Lane 5:

293T cell lysates at 10 µg

Lane 6:

Jurkat cell lysates at 10 µg

Predicted band size: 267 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8982

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249609 is the carrier-free version of ab170940.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The translocated promoter region protein (TPR) also known commonly as 'horse TPR' consists of approximately 267 kDa. TPR is a nucleoporin protein primarily expressed in the nuclear envelope of eukaryotic cells. It serves as a structural and regulatory component of the nuclear pore complex (NPC) playing a critical role in nucleocytoplasmic transport. A structural scaffold TPR facilitates the organization and entrance of nuclear transport receptors and cargo molecules through the NPC impacting the efficient movement of proteins and RNA across the nuclear membrane.
Biological function summary

TPR interacts with other nucleoporins within the NPC to ensure proper nuclear function. TPR is integral in maintaining the spatial organization and transport selectivity within the nucleoplasm-cytoplasm interface by forming an interaction network with other proteins like NUP98 and NUP153. TPR functions as part of the NPC assembly contributing to essential cellular processes like gene expression regulation and DNA repair by participating in chromatin organization.

Pathways

TPR integrates into the nuclear transport pathway and it also plays a role in the MAPK signaling pathway. Within the nuclear transport pathway TPR facilitates the proper translocation of molecules necessary for downstream cellular functions. In the MAPK signaling pathway it synchronizes with other proteins like ERK to control processes such as proliferation and differentiation. TPR's interaction with these pathways positions it as an important player in cellular communication and response.

TPR has connections to cancer and neurodegenerative diseases. In various cancers abnormal TPR function impacts cell growth and proliferation by altering nuclear transport dynamics. Additionally it associates with Alzheimer's disease through its interactions with tau protein affecting neuronal function. These connections make TPR a significant target for understanding disease mechanisms and developing therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. According to some authors, plays a limited role in the regulation of nuclear protein export (PubMed : 11952838, PubMed : 22253824). Also plays a role as a structural and functional element of the perinuclear chromatin distribution; involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases.
See full target information Nucleoprotein TPR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5206 PubMed39959296

2025

Cell-Sonar, an Easy and Low-cost Method to Track a Target Protein by Expression Changes of Specific Protein Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Lara Maria Molitor,Franz Worek,Simone Rothmiller
View all publications
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