Anti-TPSB2 antibody [EPR28111-57]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TPSB2 antibody [EPR28111-57] (AB314321)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-937 (human histiocytic lymphoma monocyte) cells labelling TPSB2 with ab314321 at 1/100 (5.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in U-937 cell line.Negative control : K-562 (PMID : 8210998) Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• WB

Supplier Data

Western blot - Anti-TPSB2 antibody [EPR28111-57] (AB314321)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8210998, 23222722). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TPSB2 antibody [EPR28111-57] (ab314321) at 1/1000 dilution

Lane 1:

Human colon cancer tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Lane 3:

Human tonsil tissue lysate at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 30-36 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-TPSB2 antibody [EPR28111-57] (AB314321)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : K562 (PMID : 8210998). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8210998, 23222722). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TPSB2 antibody [EPR28111-57] (ab314321) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30-36 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-TPSB2 antibody [EPR28111-57] (AB314321)

Blocking and diluting buffer and concentration : 5% NFDM/TBST TPSB2 is a glycoprotein of approximately 36 kDa and detected as a 30kDa band after treated with Protein Deglycosylation Peptide : N-glycosidase F (PNGase F). In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-TPSB2 antibody [EPR28111-57] (ab314321) at 1/1000 dilution

Lane 1:

Untreated U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

U937 whole cell lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa,36 kDa

false

Exposure time: 81s