AB249299

Anti-TPST2 antibody [EPR10569] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TPST2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

Protein-tyrosine sulfotransferase 2, Tyrosylprotein sulfotransferase 2, TPST-2, TPST2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] - BSA and Azide free (AB249299)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] - BSA and Azide free (AB249299)

This data was developed using ab157191, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TPST2 with ab157191 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] - BSA and Azide free (AB249299)

This data was developed using ab157191, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TPST2 with ab157191 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TPST2 antibody [EPR10569] - BSA and Azide free (AB249299)

This data was developed using ab157191, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TPST2 antibody [EPR10569] (<a href='/en-us/products/primary-antibodies/tpst2-antibody-epr10569-ab157191'>ab157191</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 21 kDa,42 kDa

false

Unconjugated

Anti-TPST2 antibody [EPR10569]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10569

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249299 is the carrier-free version of ab157191.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPST2 also known as tyrosylprotein sulfotransferase 2 is an enzyme that transfers a sulfate group to the tyrosine residues of proteins. It has an approximate mass of 54 kDa. TPST2 is expressed in various tissues including the pancreas thyroid and lung. The activity of TPST2 is essential for the post-translational modification process which is important for protein function and interaction.

Biological function summary

Tyrosine sulfation by TPST2 enhances protein-protein interactions impacting various cellular processes. TPST2 often forms a part of larger enzyme complexes within the Golgi apparatus facilitating the sulfation of substrates. This post-translational modification is important for modulating the activity and binding affinity of many proteins with consequences on cell signaling and communication.

Pathways

The enzyme TPST2 participates in the sulfation pathway impacting protein function and regulation within cells. It works alongside proteins such as heparan sulfate proteoglycans (HSPGs) in modulating biological processes. Tyrosine sulfation conducted by TPST2 plays a role in the immune response pathway assisting in the regulation of leukocyte adhesion and signaling.

Aberrations in TPST2 expression or function can connect to several conditions. Research has indicated potential links between TPST2 and inflammatory disorders. Disorders like rheumatoid arthritis may show association due to altered sulfation patterns impacting immune cell interactions. TPST2 has also been studied in relation to cancer where modification of cell surface proteins may influence tumor progression and metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.

See full target information TPST2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TPST2 antibody [EPR10569] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] - BSA and Azide free (AB249299)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] - BSA and Azide free (AB249299)

Western blot - Anti-TPST2 antibody [EPR10569] - BSA and Azide free (AB249299)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise