Anti-TPTE antibody [EPR11867(2)] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TPTE antibody [EPR11867(2)] - BSA and Azide free (AB250148)

This data was developed using ab180137, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed PC3 cells labeling TPTE with ab180137 at 1/10 dilution. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPTE antibody [EPR11867(2)] - BSA and Azide free (AB250148)

This data was developed using ab180137, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TPTE with ab180137 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TPTE antibody [EPR11867(2)] - BSA and Azide free (AB250148)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue. Panel A : Merged staining of anti-IGF2BP1/IMP1 (green; Opal™690), anti-TPTE (gray; Opal™520) and anti-SAGE1 (red; Opal™570) on human testis. Panel B : Anti-IGF2BP1/IMP1 stained on cytoplasm of spermatogonia. Panel C : Anti-TPTE stained on spermatocytes. Panel D : Anti-SAGE1 stained on nucleus of spermatogonia. The section was incubated in three rounds of staining : in the order of ab290736, ab250148, and ab233388 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TPTE antibody [EPR11867(2)] - BSA and Azide free (AB250148)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue. Panel A : Merged staining of anti-AKAP4 (magenta; Opal™690), anti-TPTE (green; Opal™520) and anti-IGF2BP1/IMP1 (red; Opal™570) on human testis. Panel B : Anti-IGF2BP1/IMP1 stained on spermatogonia. Panel C : Anti-TPTE stained on spermatocytes. Panel D : Anti-AKAP4 stained on spermatids. The section was incubated in three rounds of staining : in the order of ab238887, ab250148, and ab290736 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.