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AB264124

Anti-TPX2 antibody

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Rabbit Polyclonal TPX2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TPX2 aa 150-200.

View Alternative Names

C20orf1, C20orf2, DIL2, HCA519, TPX2, Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, DIL-2, p100

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody (AB264124)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody (AB264124)

Formaldehyde-fixed asynchronous HeLa (Human epithelial cell line from cervix adenocarcinoma) cell stained for TPX2 using ab264124 at a 1/400 dilution in ICC/IF. A red-fluorescent goat anti-rabbit IgG heavy and light chain, cross-adsorbed DyLight® 594 conjugated secondary antibody was used at a dilution of 1/100.

Immunoprecipitation - Anti-TPX2 antibody (AB264124)
  • IP

Supplier Data

Immunoprecipitation - Anti-TPX2 antibody (AB264124)

TPX2 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264124 at 0.4μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264124 at 0.1μg/ml.
Lane 1 : HeLa whole cell lysate 50μg (Input).
Lane 2 : ab264124 IP in HeLa whole cell lysate.
Lane 3 : ab264124 IP in HeLa whole cell lysate plus blocking peptide.

Exposure time : 1 min.

All lanes:

Immunoprecipitation - Anti-TPX2 antibody (ab264124)

Predicted band size: 86 kDa

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Western blot - Anti-TPX2 antibody (AB264124)
  • WB

Supplier Data

Western blot - Anti-TPX2 antibody (AB264124)

4-8% SDS-PAGE.

All lanes:

Western blot - Anti-TPX2 antibody (ab264124) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Predicted band size: 86 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TPX2 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q9ULW0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/1000", "ICCIF-species-notes": "<p>Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Targeting Protein for Xklp2 (TPX2) also known as Microtubule-Associated Protein Required for Spindle acts mechanically in the assembly of mitotic spindles. With a molecular mass of approximately 82 kDa TPX2 localizes to the nucleus in interphase and distributes along the spindle microtubules during mitosis. Its expression is high in actively dividing cells such as those found in developing tissues and proliferative zones. This association with microtubules positions TPX2 as a critical player in cell division.
Biological function summary

TPX2 contributes to the proper regulation and progression of mitosis. As part of the mitotic spindle apparatus TPX2 interacts with other spindle assembly factors to facilitate the nucleation and stabilization of microtubules. It orchestrates the proper arrangement of spindle poles ensuring accurate chromosome segregation. TPX2 operates within a complex that includes proteins such as Aurora A kinase which further accentuates its role in spindle dynamics and mitotic progression.

Pathways

TPX2 interacts intricately with the cell cycle and mitotic spindle checkpoint pathways. As an important regulator in these pathways it works alongside proteins like Aurora A kinase and Eg5 to control spindle formation and function which are necessary for the G2/M phase transition. These pathways ensure that cell division proceeds only when all chromosomal alignments and spindle assemblies meet the required fidelity underlining the importance of TPX2 in maintaining genomic stability.

The overexpression of TPX2 often correlates with several types of cancer including breast and pancreatic cancers. Aberrant TPX2 levels disturb normal mitosis contributing to aneuploidy and tumor progression. Furthermore TPX2 overactivity can interact with oncogenic proteins such as MYC enhancing tumorigenic potential. Understanding TPX2's role in these diseases offers potential targets for therapeutic intervention making it an important subject of study in cancer research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed : 18663142, PubMed : 19208764, PubMed : 37728657). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed : 18663142, PubMed : 19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed : 26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activate AURKA kinase and stimulate local microtubule nucleation (PubMed : 26165940).
See full target information TPX2
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