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Rabbit Recombinant Monoclonal TPX2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23182-47] (AB252944), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23182-47] (AB252944), expandable thumbnail
  • Western blot - Anti-TPX2 antibody [EPR23182-47] (AB252944), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (AB252944), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TPX2 antibody [EPR23182-47] (AB252944), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-PIP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Tested
Expected
Tested
Not recommended
Rat
Tested
Expected
Expected
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764, PubMed:37728657). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activate AURKA kinase and stimulate local microtubule nucleation (PubMed:26165940).

Alternative names

C20orf1, C20orf2, DIL2, HCA519, TPX2, Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, DIL-2, p100

Recommended products

Rabbit Recombinant Monoclonal TPX2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23182-47
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Targeting Protein for Xklp2 (TPX2) also known as Microtubule-Associated Protein Required for Spindle acts mechanically in the assembly of mitotic spindles. With a molecular mass of approximately 82 kDa TPX2 localizes to the nucleus in interphase and distributes along the spindle microtubules during mitosis. Its expression is high in actively dividing cells such as those found in developing tissues and proliferative zones. This association with microtubules positions TPX2 as a critical player in cell division.

Biological function summary

TPX2 contributes to the proper regulation and progression of mitosis. As part of the mitotic spindle apparatus TPX2 interacts with other spindle assembly factors to facilitate the nucleation and stabilization of microtubules. It orchestrates the proper arrangement of spindle poles ensuring accurate chromosome segregation. TPX2 operates within a complex that includes proteins such as Aurora A kinase which further accentuates its role in spindle dynamics and mitotic progression.

Pathways

TPX2 interacts intricately with the cell cycle and mitotic spindle checkpoint pathways. As an important regulator in these pathways it works alongside proteins like Aurora A kinase and Eg5 to control spindle formation and function which are necessary for the G2/M phase transition. These pathways ensure that cell division proceeds only when all chromosomal alignments and spindle assemblies meet the required fidelity underlining the importance of TPX2 in maintaining genomic stability.

Associated diseases and disorders

The overexpression of TPX2 often correlates with several types of cancer including breast and pancreatic cancers. Aberrant TPX2 levels disturb normal mitosis contributing to aneuploidy and tumor progression. Furthermore TPX2 overactivity can interact with oncogenic proteins such as MYC enhancing tumorigenic potential. Understanding TPX2's role in these diseases offers potential targets for therapeutic intervention making it an important subject of study in cancer research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling TPX2 with ab252944 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HeLa cells. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labeling TPX2 with ab252944 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in RAW 264.7 cells. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Western blot - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:25239289, 16489064).

    The lower bands are mainly caused by degradation (PMID:16287863).

    Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 26 secs.

    All lanes: Western blot - Anti-TPX2 antibody [EPR23182-47] (ab252944) at 1/1000 dilution

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 2: L6 (rat skeletal muscle myoblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling TPX2 with ab252944 at 1/1000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proliferative cells of mouse colon (PMID:23634259). Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry (Intracellular) - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Intracellular flow cytometric analysis of 80% methanol fixed 0.1% Tween-20 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TPX2 with ab252944 at 1/50 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Western blot - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:25239289, 16489064).

    Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 3 secs.

    All lanes: Western blot - Anti-TPX2 antibody [EPR23182-47] (ab252944) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 3: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 86 kDa

    Observed band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling TPX2 with ab252944 at 1/1000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proliferative cells of rat colon (PMID:23634259). Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TPX2 with ab252944 at 1/1000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proliferative cells of human lung cancer (PMID:23634259, 16489064). Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (ab252944), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23182-47] (ab252944)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling TPX2 with ab252944 at 1/1000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proliferative cells of human colon cancer (PMID:23634259, 22207630). Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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