AB71985

Anti-TR150 antibody

Name: Anti-TR150 antibody (ab71985) | Abcam
Brand: Abcam Limited
SKU: AB71985

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(3 Publications)

Rabbit Polyclonal TR150 antibody. Suitable for WB, IHC-P and reacts with Human, Chimpanzee samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human THRAP3 aa 900 to C-terminus.

View Alternative Names

BCLAF2, TRAP150, THRAP3, Thyroid hormone receptor-associated protein 3, BCLAF1 and THRAP3 family member 2, Thyroid hormone receptor-associated protein complex 150 kDa component, Trap150

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR150 antibody (AB71985)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR150 antibody (AB71985)

Paraffin embedded human colon tissue immunostained with ab71985 at 10 μg/ml.

Unknown

Western blot - Anti-TR150 antibody (AB71985)

All lanes:

Western blot - Anti-TR150 antibody (ab71985) at 0.1 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

HeLa cell lysate with immunising peptide

Predicted band size: 109 kDa

Observed band size: 109 kDa,140 kDa,40 kDa,60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Chimpanzee

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human THRAP3 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TR150 also known as Transcription Regulator 150 is a protein with a molecular mass of approximately 150 kDa. You can find its expression in various tissues with higher levels in liver and brain tissues. Mechanically TR150 acts as a transcriptional regulator and binds directly to DNA to modulate the expression of different genes. Its capability to bind specific DNA sequences allows it to influence gene transcription effectively.

Biological function summary

TR150 plays a critical role in regulating gene expression that affects cellular functions like cell cycle progression and cell differentiation. It functions as part of a transcriptional regulatory complex interacting with cofactors and other transcriptional regulators to fine-tune gene expression. Its activity impacts cellular growth and development indicating its integral role in maintaining cellular homeostasis.

Pathways

TR150 is involved in the cell cycle regulation pathway and the Wnt signaling pathway. In the cell cycle pathway TR150 works alongside proteins such as Cyclin D1 to control cell division. In the Wnt signaling pathway it interacts with β-catenin and influences the transcriptional activation of Wnt target genes. Through these pathways TR150 helps in the coordination of proliferative signals and gene expression patterns.

Altered expression of TR150 has been linked to cancer and neurodegenerative diseases. Overexpression or dysfunction in TR150 can disrupt normal cell cycle control contributing to oncogenesis. In neurodegenerative diseases abnormal TR150 expression may interfere with neuronal function and survival. In these contexts it connects to proteins like p53 in cancer pathways and Tau in neurodegenerative pathways highlighting its involvement in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes (PubMed : 24043798).

See full target information THRAP3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 22:986-1004 PubMed36762777

2023

Metformin attenuates multiple myeloma cell proliferation and encourages apoptosis by suppressing METTL3-mediated m6A methylation of THRAP3, RBM25, and USP4.

Applications

Unspecified application

Species

Unspecified reactive species

Cong-Jie Chen,Jie-Yun Huang,Jian-Qing Huang,Jia-Yi Deng,Xiao-Hui Shangguan,Ai-Zhen Chen,Long-Tian Chen,Wei-Hao Wu

Proceedings of the National Academy of Sciences of the United States of America 110:16021-6 PubMed24043798

2013

A positive feedback loop links circadian clock factor CLOCK-BMAL1 to the basic transcriptional machinery.

Applications

IP, IP

Species

Mouse, Human

Laura Lande-Diner,Cyril Boyault,Jin Young Kim,Charles J Weitz

Molecular cell 40:126-37 PubMed20932480

2010

Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing.

Applications

Species

Human

Florian Heyd,Kristen W Lynch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TR150 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR150 antibody (AB71985)

Western blot - Anti-TR150 antibody (AB71985)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise