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AB72455

Anti-TR150 antibody

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Rabbit Polyclonal TR150 antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human THRAP3.

View Alternative Names

BCLAF2, TRAP150, THRAP3, Thyroid hormone receptor-associated protein 3, BCLAF1 and THRAP3 family member 2, Thyroid hormone receptor-associated protein complex 150 kDa component, Trap150

1 Images
Immunoprecipitation - Anti-TR150 antibody (AB72455)
  • IP

Unknown

Immunoprecipitation - Anti-TR150 antibody (AB72455)

HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab72455 used for IP at 3 µg/mg lysate. For subsequent Western blotting of immunoprecipitated TR150, another antibody to TR150 was used at 0.1 mcg/ml.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TR150 antibody (ab72455)

Predicted band size: 109 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human THRAP3. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2W1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TR150 also known as Transcription Regulator 150 is a protein with a molecular mass of approximately 150 kDa. You can find its expression in various tissues with higher levels in liver and brain tissues. Mechanically TR150 acts as a transcriptional regulator and binds directly to DNA to modulate the expression of different genes. Its capability to bind specific DNA sequences allows it to influence gene transcription effectively.
Biological function summary

TR150 plays a critical role in regulating gene expression that affects cellular functions like cell cycle progression and cell differentiation. It functions as part of a transcriptional regulatory complex interacting with cofactors and other transcriptional regulators to fine-tune gene expression. Its activity impacts cellular growth and development indicating its integral role in maintaining cellular homeostasis.

Pathways

TR150 is involved in the cell cycle regulation pathway and the Wnt signaling pathway. In the cell cycle pathway TR150 works alongside proteins such as Cyclin D1 to control cell division. In the Wnt signaling pathway it interacts with β-catenin and influences the transcriptional activation of Wnt target genes. Through these pathways TR150 helps in the coordination of proliferative signals and gene expression patterns.

Altered expression of TR150 has been linked to cancer and neurodegenerative diseases. Overexpression or dysfunction in TR150 can disrupt normal cell cycle control contributing to oncogenesis. In neurodegenerative diseases abnormal TR150 expression may interfere with neuronal function and survival. In these contexts it connects to proteins like p53 in cancer pathways and Tau in neurodegenerative pathways highlighting its involvement in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes (PubMed : 24043798).
See full target information THRAP3
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