Rabbit Polyclonal TR4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR2C2 aa 50-400.
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Publications
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- International journal of molecular medicine 44:885-8922019HMGB1 enhances mechanical stress-induced cardiomyocyte hypertrophy in vitro via the RAGE/ERK1/2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhang et. al.PubMed 31524228
- Biochemical and biophysical research communications 508:210-2162018Role and mechanism of miR-4778-3p and its targets NR2C2 and Med19 in cervical cancer radioresistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhang,Pei Li,Jing Hu,Li Na Zhao,Jian Ping Li,Rui Ma,Wei Wei Li,Mei Shi,Li Chun WeiPubMed 30473219
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NR2C2 aa 50-400. The exact immunogen used to generate this antibody is proprietary information. Database link P49116
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
Alternative names
TAK1, TR4, NR2C2, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4
Recommended products
Rabbit Polyclonal TR4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR2C2 aa 50-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NR2C2 aa 50-400. The exact immunogen used to generate this antibody is proprietary information. Database link P49116
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TR4 also known as testicular receptor 4 or NR2C2 is a nuclear receptor that belongs to the steroid hormone receptor superfamily. It acts as a transcription factor regulating the expression of specific genes. TR4 has a molecular weight of approximately 68 kDa. We find TR4 expression in various tissues including the testis prostate and kidney reflecting its diverse roles in different cellular processes.
- Biological function summary
TR4 significantly influences reproductive development and metabolic processes. It plays an important role in regulating genes involved in lipid and glucose metabolism. TR4 does not function alone; it often forms complexes with other proteins to exert its effects on gene expression. The formation of these complexes enhances its ability to control a wide range of biological activities.
- Pathways
TR4 actively participates in the insulin signaling and androgen receptor pathways. It modulates the action of insulin by influencing genes associated with glucose metabolism impacting energy homeostasis. In the androgen receptor pathway TR4 shares regulatory roles with proteins like AR and NR2C1 ensuring proper development and function of reproductive organs. Its involvement in these pathways exemplifies its role in maintaining metabolic and reproductive health.
- Associated diseases and disorders
Scientists link TR4 with metabolic syndrome and prostate cancer. Abnormal TR4 expression or function associates with disrupted glucose and lipid metabolism contributing to metabolic syndrome. In prostate cancer TR4 interacts with the androgen receptor affecting tumor growth and progression. Understanding these connections helps in exploring TR4 as a potential therapeutic target for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-TR4 antibody (ab137582)
7.5% SDS-PAGE
All lanes: Western blot - Anti-TR4 antibody (ab137582) at 1/500 dilution
Lane 1: H1299 whole cell lysate at 30 µg
Lane 2: Molt-4 whole cell lysate at 30 µg
Predicted band size: 65 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR4 antibody (ab137582)
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft labelling TR4 with ab137582 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TR4 antibody (ab137582)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labelling TR4 with ab137582 at 1/500 dilution.
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