JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB137582

Anti-TR4 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal TR4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR2C2 aa 50-400.

View Alternative Names

TAK1, TR4, NR2C2, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR4 antibody (AB137582)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR4 antibody (AB137582)

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft labelling TR4 with ab137582 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TR4 antibody (AB137582)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TR4 antibody (AB137582)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labelling TR4 with ab137582 at 1/500 dilution.

Western blot - Anti-TR4 antibody (AB137582)
  • WB

Unknown

Western blot - Anti-TR4 antibody (AB137582)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-TR4 antibody (ab137582) at 1/500 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NR2C2 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

P49116

style
left-column

Alternative Products

Primary Antibodies

AB109301

Anti-TR4 antibody [EPR1773(2)]

RabMAb|Recombinant

Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (AB109301)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 19
Primary Antibodies

AB248451

Anti-Prostate Secretory Protein/PSP antibody [EPR7346] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Prostate Secretory Protein/PSP antibody [EPR7346] - BSA and Azide free (AB248451)

  • 0
  • 1
Primary Antibodies

AB247896

Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] - BSA and Azide free (AB247896)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TR4 also known as testicular receptor 4 or NR2C2 is a nuclear receptor that belongs to the steroid hormone receptor superfamily. It acts as a transcription factor regulating the expression of specific genes. TR4 has a molecular weight of approximately 68 kDa. We find TR4 expression in various tissues including the testis prostate and kidney reflecting its diverse roles in different cellular processes.
Biological function summary

TR4 significantly influences reproductive development and metabolic processes. It plays an important role in regulating genes involved in lipid and glucose metabolism. TR4 does not function alone; it often forms complexes with other proteins to exert its effects on gene expression. The formation of these complexes enhances its ability to control a wide range of biological activities.

Pathways

TR4 actively participates in the insulin signaling and androgen receptor pathways. It modulates the action of insulin by influencing genes associated with glucose metabolism impacting energy homeostasis. In the androgen receptor pathway TR4 shares regulatory roles with proteins like AR and NR2C1 ensuring proper development and function of reproductive organs. Its involvement in these pathways exemplifies its role in maintaining metabolic and reproductive health.

Scientists link TR4 with metabolic syndrome and prostate cancer. Abnormal TR4 expression or function associates with disrupted glucose and lipid metabolism contributing to metabolic syndrome. In prostate cancer TR4 interacts with the androgen receptor affecting tumor growth and progression. Understanding these connections helps in exploring TR4 as a potential therapeutic target for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
See full target information NR2C2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 44:885-892 PubMed31524228

2019

HMGB1 enhances mechanical stress-induced cardiomyocyte hypertrophy in vitro via the RAGE/ERK1/2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Xue Yang,Guoliang Jiang,Ying Yu,Jian Wu,Yangang Su,Aijun Sun,Yunzeng Zou,Hong Jiang,Junbo Ge

Biochemical and biophysical research communications 508:210-216 PubMed30473219

2018

Role and mechanism of miR-4778-3p and its targets NR2C2 and Med19 in cervical cancer radioresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Pei Li,Jing Hu,Li Na Zhao,Jian Ping Li,Rui Ma,Wei Wei Li,Mei Shi,Li Chun Wei
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com