Rabbit Recombinant Monoclonal TR4 antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 10 publications.
Images
![Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (AB109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--immunoprecipitation-img16248.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (AB109301)")
![Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img150339.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)")
![Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img101018.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)")
![Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img56560.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)")
![Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img83643.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)")
Publications
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- Nature communications 14:17322023A regulatory variant at 19p13.3 is associated with primary biliary cholangitis risk and ARID3A expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou Li,Zhiqiang Li,Ruiling Chen,Min Lian,Hanxiao Wang,Yiran Wei,Zhengrui You,Jun Zhang,Bo Li,Yikang Li,Bingyuan Huang,Yong Chen,Qiaoyan Liu,Zhuwan Lyu,Xueying Liang,Qi Miao,Xiao Xiao,Qixia Wang,Jingyuan Fang,YongYong Shi,Xiangdong Liu,Michael F Seldin,M Eric Gershwin,Ruqi Tang,Xiong MaPubMed 36977669
- Frontiers in molecular biosciences 8:6704092021Testicular Nuclear Receptor 4 Regulates Proliferation and Apoptosis of Bladder Cancer Bcl-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Wang,Wenqin Luo,Xuliang Wang,Dingwei Xue,Liangliang Ren,Li Xu,Guangju Ge,Liqun Xia,Shicheng Yu,Mingchao Wang,Zhenwei Zhou,Gonghui Li,Haiyang WuPubMed 34616769
- Journal of cellular and molecular medicine 25:2404-24172021Targeting the TR4 nuclear receptor with antagonist bexarotene can suppress the proopiomelanocortin signalling in AtT-20 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiqun Xia,Danyang Shen,Youyun Zhang,Jieyang Lu,Mingchao Wang,Huan Wang,Yuanlei Chen,Dingwei Xue,Dajiang Xie,Gonghui LiPubMed 33491272
- Journal of assisted reproduction and genetics :2020Wnt pathway modulation generates blastomere-derived mouse embryonic stem cells with different pluripotency features.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Vila-Cejudo et. al.PubMed 33047186
- Oncogene 39:1891-19032019Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinyi Hu,Yin Sun,Jie Luo,Xiang He,Meihua Ye,Gonghui Li,Yong Zhang,Jian Bai,Dahong Zhang,Chawnshang ChangPubMed 31748715
- Oncogene 39:530-5452019Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHangchuan Shi,Yin Sun,Miao He,Xiong Yang,Michiaki Hamada,Tsukasa Fukunaga,Xiaoping Zhang,Chawnshang ChangPubMed 31501521
- International journal of cancer 143:100-1122018Preclinical studies using miR-32-5p to suppress clear cell renal cell carcinoma metastasis via altering the miR-32-5p/TR4/HGF/Met signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingchao Wang,Yin Sun,Junjie Xu,Jieyang Lu,Kefeng Wang,Dong-Rong Yang,Guosheng Yang,Gonghui Li,Chawnshang ChangPubMed 29396852
- Cancer science 109:384-3942017Testicular orphan receptor 4 promotes tumor progression and implies poor survival through AKT3 regulation in seminoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanlei Chen,Jieyang Lu,Liqun Xia,Dingwei Xue,Xiaoming Yu,Danyang Shen,Liwei Xu,Gonghui LiPubMed 29197138
- Hormone molecular biology and clinical investigation 29:13-26201617β-Estradiol suppresses visceral adipogenesis and activates brown adipose tissue-specific gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaad Misfer Al-Qahtani,Galyna Bryzgalova,Ismael Valladolid-Acebes,Marion Korach-André,Karin Dahlman-Wright,Suad Efendić,Per-Olof Berggren,Neil PortwoodPubMed 27831918
- Oncotarget 7:69149-691582016Targeting the ERK pathway for the treatment of Cushing's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongyun Zhang,Marvin Bergsneider,Marilene B Wang,Anthony P HeaneyPubMed 27708250
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended |
| Mouse | Not recommended | Expected | Tested | Not recommended |
| Rat | Not recommended | Expected | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10 - 1/100 | Notes - |
Species Mouse | Dilution info 1/10 - 1/100 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
Alternative names
TAK1, TR4, NR2C2, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4
Recommended products
Rabbit Recombinant Monoclonal TR4 antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR1773(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TR4 also known as testicular receptor 4 or NR2C2 is a nuclear receptor that belongs to the steroid hormone receptor superfamily. It acts as a transcription factor regulating the expression of specific genes. TR4 has a molecular weight of approximately 68 kDa. We find TR4 expression in various tissues including the testis prostate and kidney reflecting its diverse roles in different cellular processes.
- Biological function summary
TR4 significantly influences reproductive development and metabolic processes. It plays an important role in regulating genes involved in lipid and glucose metabolism. TR4 does not function alone; it often forms complexes with other proteins to exert its effects on gene expression. The formation of these complexes enhances its ability to control a wide range of biological activities.
- Pathways
TR4 actively participates in the insulin signaling and androgen receptor pathways. It modulates the action of insulin by influencing genes associated with glucose metabolism impacting energy homeostasis. In the androgen receptor pathway TR4 shares regulatory roles with proteins like AR and NR2C1 ensuring proper development and function of reproductive organs. Its involvement in these pathways exemplifies its role in maintaining metabolic and reproductive health.
- Associated diseases and disorders
Scientists link TR4 with metabolic syndrome and prostate cancer. Abnormal TR4 expression or function associates with disrupted glucose and lipid metabolism contributing to metabolic syndrome. In prostate cancer TR4 interacts with the androgen receptor affecting tumor growth and progression. Understanding these connections helps in exploring TR4 as a potential therapeutic target for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--immunoprecipitation-img16248.jpg "Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)
ab109301 (purified) at 1/30 immunoprecipitating TR4 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)
Predicted band size: 65 kDa
![Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img150339.jpg "Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
Lanes 1-3: Merged signal (red and green). Green - ab109301 observed at 67 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in knockout cell line Human NR2C2 (TR4) knockout HEK-293T cell line ab266228 (knockout cell lysate Human NR2C2 (TR4) knockout HEK-293T cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 knockout samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: NR2C2 knockout HEK293T cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa
![Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img101018.jpg "Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
Lanes 1-3: Merged signal (red and green). Green - ab109301 observed at 67 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line Human NR2C2 (TR4) knockout HEK-293T cell line ab266228 (CRISPR/Cas9 edited cell lysate Human NR2C2 (TR4) knockout HEK-293T cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: NR2C2 CRISPR/Cas9 edited HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human NR2C2 (TR4) knockout HEK-293T cell line (Human NR2C2 (TR4) knockout HEK-293T cell line ab266228)
Lane 3: HeLa cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa
![Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img56560.jpg "Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution
Lane 1: PC-12 whole cell lysate at 20 µg
Lane 2: 3T3-L1 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa
![Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img83643.jpg "Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution
All lanes: HEK-293 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa
![Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img146539.jpg "Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
All lanes: Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution
All lanes: PC-3 whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa
![Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img57680.jpg "Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
All lanes: Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution
Lane 1: PC3 cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Predicted band size: 12 kDa, 134 kDa, 14 kDa, 37 kDa, 52 kDa, 55 kDa, 65 kDa, 96 kDa
Observed band size: 14 kDa, 67 kDa
![Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img146539.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
![Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301), expandable thumbnail](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img57680.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)")
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