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Mouse Monoclonal TRA-1-60 (R) antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 138 publications.


Images

Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288), expandable thumbnail
  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288), expandable thumbnail
  • Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288), expandable thumbnail
  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288), expandable thumbnail

Publications

Key facts

Isotype

IgM

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWB
Human
Tested
Tested
Rabbit
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Predicted
Predicted

Species

Rabbit

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-10.00000 µg/mL

Notes

-

Predicted
Predicted

Species

Rabbit

Dilution info

-

Notes

-

Alternative names

Recommended products

Mouse Monoclonal TRA-1-60 (R) antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 138 publications.

Key facts

Isotype

IgM

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

TRA-1-60

Purity

Tissue culture supernatant

Concentration
Loading...
Purification notes

Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TRA-1-60 also known as TRA-1-60 (R) is an antigenic target associated with cell surface glycoproteins and stem cell markers. It has a molecular mass of approximately 200 kDa and is known to be expressed on the surface of embryonic stem cells and germ cell tumors. This target along with its related antibodies such as anti-TRA-1-60 and anti-TRA serves as a marker for undifferentiated human embryonic stem cells and is used extensively in stem cell research to identify and isolate pluripotent cells.

Biological function summary

TRA-1-60 plays a significant role in the maintenance of stem cell pluripotency and self-renewal. It is a carbohydrate epitope on the cell surface and part of a larger complex that includes distinct markers such as SSEA-3 and SSEA-4. This complex is important in the signaling pathways that help preserve the undifferentiated state of stem cells enabling them to differentiate into various cell types as needed during development and tissue regeneration.

Pathways

TRA-1-60 participates in key pathways that regulate cell development and differentiation notably the Wnt and TGF-beta pathways. These pathways are essential for maintaining stem cell pluripotency and are connected to related proteins such as beta-catenin in the Wnt pathway and SMAD proteins in the TGF-beta pathway. TRA-1-60 interacts with these proteins to support the delicate balance between cell differentiation and self-renewal.

Associated diseases and disorders

TRA-1-60 has associations with testicular germ cell tumors and teratomas. The expression of TRA-1-60 in such tumors correlates with tumorigenic potential and is often used as a diagnostic marker. In these diseases TRA-1-60 associates with proteins like PLAP (placental alkaline phosphatase) which is also a marker for germ cell tumors. The presence of TRA-1-60 provides insights into the differentiation state of the tumor cells and helps in monitoring disease progression.

Product promise

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4 product images

  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288), expandable thumbnail
    This image is courtesy of a customer review submitted by Ms Fiona Lewis

    Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

    Immunocytochemical analysis of ab16288 staining TRA-160 (R) in HUES7 cells. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1:500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgM, dilution 1:100, was used as secondary antibody.

  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288), expandable thumbnail
    This image is courtesy of an anonymous collaborator.

    Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

    This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody Anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.

  • Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288), expandable thumbnail

    Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

    As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.

    All lanes: Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/mL

    Lane 1: Human Embryonic Stem Cell Lysate at 20 µg

    Lane 2: Mouse Embryonic Stem Cell Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) ab5930) at 1/5000 dilution

    Predicted band size: 235 kDa

    Observed band size: 250 kDa

  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288), expandable thumbnail
    This image is courtesy of a customer review submitted by Vladimir Milenkovic, University Hospital Regensburg, Germany

    Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)



    Immunocytochemistry analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1:500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1:500 dilution.

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Product protocols

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