Mouse Monoclonal TRA-1-60 (R) antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 138 publications.
IgM
Mouse
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Liquid
Monoclonal
ICC | WB | |
---|---|---|
Human | Tested | Tested |
Rabbit | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-10.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit | Dilution info - | Notes - |
TRA 1 60
Mouse Monoclonal TRA-1-60 (R) antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 138 publications.
IgM
Mouse
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Liquid
Monoclonal
TRA-1-60
Tissue culture supernatant
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
TRA-1-60 also known as TRA-1-60 (R) is an antigenic target associated with cell surface glycoproteins and stem cell markers. It has a molecular mass of approximately 200 kDa and is known to be expressed on the surface of embryonic stem cells and germ cell tumors. This target along with its related antibodies such as anti-TRA-1-60 and anti-TRA serves as a marker for undifferentiated human embryonic stem cells and is used extensively in stem cell research to identify and isolate pluripotent cells.
TRA-1-60 plays a significant role in the maintenance of stem cell pluripotency and self-renewal. It is a carbohydrate epitope on the cell surface and part of a larger complex that includes distinct markers such as SSEA-3 and SSEA-4. This complex is important in the signaling pathways that help preserve the undifferentiated state of stem cells enabling them to differentiate into various cell types as needed during development and tissue regeneration.
TRA-1-60 participates in key pathways that regulate cell development and differentiation notably the Wnt and TGF-beta pathways. These pathways are essential for maintaining stem cell pluripotency and are connected to related proteins such as beta-catenin in the Wnt pathway and SMAD proteins in the TGF-beta pathway. TRA-1-60 interacts with these proteins to support the delicate balance between cell differentiation and self-renewal.
TRA-1-60 has associations with testicular germ cell tumors and teratomas. The expression of TRA-1-60 in such tumors correlates with tumorigenic potential and is often used as a diagnostic marker. In these diseases TRA-1-60 associates with proteins like PLAP (placental alkaline phosphatase) which is also a marker for germ cell tumors. The presence of TRA-1-60 provides insights into the differentiation state of the tumor cells and helps in monitoring disease progression.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Immunocytochemical analysis of ab16288 staining TRA-160 (R) in HUES7 cells. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1:500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgM, dilution 1:100, was used as secondary antibody.
This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody Anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.
All lanes: Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/mL
Lane 1: Human Embryonic Stem Cell Lysate at 20 µg
Lane 2: Mouse Embryonic Stem Cell Lysate at 20 µg
All lanes: Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) ab5930) at 1/5000 dilution
Predicted band size: 235 kDa
Observed band size: 250 kDa
Immunocytochemistry analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1:500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1:500 dilution.
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