Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker

• WB

Lab

Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (AB16289)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16289 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/mL

All lanes:

HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 59 kDa

true

Exposure time: 20min

• WB

Unknown

Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (AB16289)

All lanes:

Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/mL

All lanes:

MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (<a href='/en-us/products/unavailable/mel-2-human-embryonic-stem-cell-female-cell-line-whole-cell-lysate-ab27196'>ab27196</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (<a href='/en-us/products/secondary-antibodies/goat-fab2-mouse-igm-mu-chain-hrp-ab5930'>ab5930</a>) at 1/5000 dilution

Predicted band size: 59 kDa

true

Exposure time: 3min