AB138448

Anti-TRA2A antibody

Name: Anti-TRA2A antibody (ab138448) | Abcam
Brand: Abcam Limited
SKU: AB138448
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal TRA2A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TRA2A aa 1-50.

View Alternative Names

Transformer-2 protein homolog alpha, TRA-2 alpha, TRA2-alpha, Transformer-2 protein homolog A, TRA2A

3 Images

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Immunoprecipitation - Anti-TRA2A antibody (AB138448)

Immunoprecipitation detection of TRA2A in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138448 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138448 at 0.4µg/ml. Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TRA2A antibody (ab138448)

Predicted band size: 33 kDa

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Supplier Data

Immunoprecipitation - Anti-TRA2A antibody (AB138448)

ab138448 at 6 μg/mg of lysate immunoprecipitating TRA2A in HeLa (human cervix adenocarcinoma) whole cell lysate.

Lane 1 : ab137248 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)

Lane 2 : ab138448 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)

Lane 3 (-) : Control IgG + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)

For western blotting, ab138448 at 0.4 μg/mL

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TRA2A antibody (ab138448)

Predicted band size: 33 kDa

false

Unknown

Western blot - Anti-TRA2A antibody (AB138448)

All lanes:

Western blot - Anti-TRA2A antibody (ab138448) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

293T cell lysate at 50 µg

Lane 4:

Jurkat cell lysate at 50 µg

Predicted band size: 33 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human TRA2A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q13595

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Baboon": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRA2A also known as transformer 2 alpha homolog is an RNA-binding protein involved in numerous cellular processes. This protein has a molecular weight of approximately 31 kDa. It is expressed in many tissues including the brain and testes. TRA2A binds to specific RNA sequences modulating splicing events and affecting the choice of splice sites. It belongs to the serine/arginine-rich protein family which plays key roles in the regulation of both alternative and constitutive splicing.

Biological function summary

TRA2A influences gene expression and transcript variants by engaging in complex interactions. It acts as part of the spliceosome machinery which is a large ribonucleoprotein complex responsible for splicing pre-mRNA. TRA2A ensures precise removal of introns and ligation of exons producing different mRNA isoforms. These isoforms translate into functionally distinct proteins influencing cellular activity.

Pathways

TRA2A participates in the mRNA processing pathway influencing genetic output by regulating splice site selection. TRA2A connects to the MAPK signaling pathway as it is known to interact with proteins like SAM68 which also affects mRNA splicing. This connectivity with other proteins highlights the regulatory network's complexity ensuring accurate gene expression profiles.

TRA2A has been implicated in cancer and neurodegenerative disorders. Altered expression and splicing activity of TRA2A contribute to oncogenesis as seen in breast cancer where abnormal splicing can drive tumor progression. TRA2A also impacts neurological conditions interplaying with proteins like tau in Alzheimer's disease where aberrant splicing plays a part in disease pathology. Understanding these links offers potential therapeutic angles for targeting TRA2A-related pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

See full target information TRA2A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 19:3678-3693 PubMed37564197

2023

Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingbo Cao,Yupeng Ren,Yuxuan Li,Junfeng Deng,Xiaorui Su,Yongchang Tang,Feng Yuan,Haixia Deng,Gaoyuan Yang,Zhiwei He,Bo Liu,Zhicheng Yao,Meihai Deng

Neoplasma 69:1322-1337 PubMed36264775

2022

Functions of long non-coding RNA LNC11649 in non-small cell lung cancer cells as a reprocessed form of MALAT1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Zhe Qi,Ting-Ting Zhao,Shu-Shan Qi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRA2A antibody

Immunoprecipitation - Anti-TRA2A antibody (AB138448)

Immunoprecipitation - Anti-TRA2A antibody (AB138448)

Western blot - Anti-TRA2A antibody (AB138448)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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