AB169052

Anti-TRA2A antibody

Name: Anti-TRA2A antibody (ab169052) | Abcam
Brand: Abcam Limited
SKU: AB169052
Price: 535 USD
Availability: InStock

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(2 Publications)

Mouse Polyclonal TRA2A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRA2A.

View Alternative Names

Transformer-2 protein homolog alpha, TRA-2 alpha, TRA2-alpha, Transformer-2 protein homolog A, TRA2A

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB169052)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB169052)

Immunofluorescent analysis of HeLa cells labeling TRA2A with ab169052 at 10 µg/ml.

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Western blot - Anti-TRA2A antibody (AB169052)

All lanes:

Western blot - Anti-TRA2A antibody (ab169052) at 1 µg/mL

All lanes:

Raw 267.4 cell lysate at 50 µg

Secondary

All lanes:

Goat anti-mouse HRP conjugated antibody at 1/2500 dilution

Predicted band size: 33 kDa

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Unknown

Western blot - Anti-TRA2A antibody (AB169052)

All lanes:

Western blot - Anti-TRA2A antibody (ab169052) at 1 µg/mL

All lanes:

HepG2 cell lysate at 50 µg

Secondary

All lanes:

Goat anti-mouse HRP conjugated antibody at 1/2500 dilution

Predicted band size: 33 kDa

true

Unknown

Western blot - Anti-TRA2A antibody (AB169052)

All lanes:

Western blot - Anti-TRA2A antibody (ab169052) at 1 µg/mL

All lanes:

NIH 3T3 cell lysate at 50 µg

Secondary

All lanes:

Goat anti-mouse HRP conjugated antibody at 1/2500 dilution

Predicted band size: 33 kDa

true

Unknown

Western blot - Anti-TRA2A antibody (AB169052)

All lanes:

Western blot - Anti-TRA2A antibody (ab169052) at 1 µg/mL

Lane 1:

TRA2A transfected 293T cell lysate at 15 µL

Lane 2:

non 293T cell lysate at 15 µL

Secondary

All lanes:

Goat anti-mouse HRP conjugated antibody at 1/2500 dilution

Predicted band size: 33 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TRA2A.

Q13595

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRA2A also known as transformer 2 alpha homolog is an RNA-binding protein involved in numerous cellular processes. This protein has a molecular weight of approximately 31 kDa. It is expressed in many tissues including the brain and testes. TRA2A binds to specific RNA sequences modulating splicing events and affecting the choice of splice sites. It belongs to the serine/arginine-rich protein family which plays key roles in the regulation of both alternative and constitutive splicing.

Biological function summary

TRA2A influences gene expression and transcript variants by engaging in complex interactions. It acts as part of the spliceosome machinery which is a large ribonucleoprotein complex responsible for splicing pre-mRNA. TRA2A ensures precise removal of introns and ligation of exons producing different mRNA isoforms. These isoforms translate into functionally distinct proteins influencing cellular activity.

Pathways

TRA2A participates in the mRNA processing pathway influencing genetic output by regulating splice site selection. TRA2A connects to the MAPK signaling pathway as it is known to interact with proteins like SAM68 which also affects mRNA splicing. This connectivity with other proteins highlights the regulatory network's complexity ensuring accurate gene expression profiles.

TRA2A has been implicated in cancer and neurodegenerative disorders. Altered expression and splicing activity of TRA2A contribute to oncogenesis as seen in breast cancer where abnormal splicing can drive tumor progression. TRA2A also impacts neurological conditions interplaying with proteins like tau in Alzheimer's disease where aberrant splicing plays a part in disease pathology. Understanding these links offers potential therapeutic angles for targeting TRA2A-related pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

See full target information TRA2A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 19:176 PubMed36329446

2022

Tripartite motif-containing protein 46 accelerates influenza A H7N9 virus infection by promoting K48-linked ubiquitination of TBK1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Su,Xian-Tian Lin,Shuai Zhao,Xiao-Qin Zheng,Yu-Qing Zhou,Lan-Lan Xiao,Hui Chen,Zheng-Yu Zhang,Li-Jun Zhang,Xiao-Xin Wu

Journal of advanced research 39:103-117 PubMed35777901

2022

miR-155 down-regulation protects the heart from hypoxic damage by activating fructose metabolism in cardiac fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Hong Zhang,Zhan Yang,Xin-Hua Zhang,Qing Miao,Min Li,Tian-Ying Zhai,Bin Zheng,Jin-Kun Wen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRA2A antibody

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB169052)

Western blot - Anti-TRA2A antibody (AB169052)

Western blot - Anti-TRA2A antibody (AB169052)

Western blot - Anti-TRA2A antibody (AB169052)

Western blot - Anti-TRA2A antibody (AB169052)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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