AB72625

Anti-TRA2A antibody

Name: Anti-TRA2A antibody (ab72625) | Abcam
Brand: Abcam Limited
SKU: AB72625
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal TRA2A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRA2A.

View Alternative Names

Transformer-2 protein homolog alpha, TRA-2 alpha, TRA2-alpha, Transformer-2 protein homolog A, TRA2A

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab72625 at 1 : 50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

Immunofluorescence analysis of Hela cells using ab72625 at a 1 : 500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

AbReview39331****

Western blot - Anti-TRA2A antibody (AB72625)

All lanes:

Western blot - Anti-TRA2A antibody (ab72625) at 1/250 dilution

Lanes 1, 3 and 5:

293T whole cell lysate at 5 µg

Lanes 2, 4 and 6:

Mouse ES (129Sv) whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 27 kDa,33 kDa,85 kDa

false

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-TRA2A antibody (AB72625)

All lanes:

Western blot - Anti-TRA2A antibody (ab72625) at 1/500 dilution

Lane 1:

Extracts from HUVEC cells at 5 µg

Lane 2:

Extracts from HUVEC cells plus 5µg immunizing peptide at 5 µg

Predicted band size: 33 kDa

Observed band size: 27 kDa,33 kDa,44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human TRA2A. The exact immunogen used to generate this antibody is proprietary information.

Q13595

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRA2A also known as transformer 2 alpha homolog is an RNA-binding protein involved in numerous cellular processes. This protein has a molecular weight of approximately 31 kDa. It is expressed in many tissues including the brain and testes. TRA2A binds to specific RNA sequences modulating splicing events and affecting the choice of splice sites. It belongs to the serine/arginine-rich protein family which plays key roles in the regulation of both alternative and constitutive splicing.

Biological function summary

TRA2A influences gene expression and transcript variants by engaging in complex interactions. It acts as part of the spliceosome machinery which is a large ribonucleoprotein complex responsible for splicing pre-mRNA. TRA2A ensures precise removal of introns and ligation of exons producing different mRNA isoforms. These isoforms translate into functionally distinct proteins influencing cellular activity.

Pathways

TRA2A participates in the mRNA processing pathway influencing genetic output by regulating splice site selection. TRA2A connects to the MAPK signaling pathway as it is known to interact with proteins like SAM68 which also affects mRNA splicing. This connectivity with other proteins highlights the regulatory network's complexity ensuring accurate gene expression profiles.

TRA2A has been implicated in cancer and neurodegenerative disorders. Altered expression and splicing activity of TRA2A contribute to oncogenesis as seen in breast cancer where abnormal splicing can drive tumor progression. TRA2A also impacts neurological conditions interplaying with proteins like tau in Alzheimer's disease where aberrant splicing plays a part in disease pathology. Understanding these links offers potential therapeutic angles for targeting TRA2A-related pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

See full target information TRA2A

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 12:4883-4890 PubMed34234858

2021

TRA2A Binds With LncRNA MALAT1 To Promote Esophageal Cancer Progression By Regulating EZH2/β-catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Zhao,Qiuyang Chen,Yujie Cai,Danze Chen,Mingrong Bei,Hongyan Dong,Jianzhen Xu

Cell reports 25:3422-3434.e7 PubMed30566867

2018

An Integrative Study of Protein-RNA Condensates Identifies Scaffolding RNAs and Reveals Players in Fragile X-Associated Tremor/Ataxia Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Cid-Samper,Mariona Gelabert-Baldrich,Benjamin Lang,Nieves Lorenzo-Gotor,Riccardo Delli Ponti,Lies-Anne W F M Severijnen,Benedetta Bolognesi,Ellen Gelpi,Renate K Hukema,Teresa Botta-Orfila,Gian Gaetano Tartaglia

Life science alliance 1:e201800070 PubMed30456350

2018

Proteomics and neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Hannelore Hartmann,Daniel Hornburg,Mareike Czuppa,Jakob Bader,Meike Michaelsen,Daniel Farny,Thomas Arzberger,Matthias Mann,Felix Meissner,Dieter Edbauer

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRA2A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

Western blot - Anti-TRA2A antibody (AB72625)

Western blot - Anti-TRA2A antibody (AB72625)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise