Rabbit Polyclonal TRA2A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRA2A.
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Publications
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- Journal of Cancer 12:4883-48902021TRA2A Binds With LncRNA MALAT1 To Promote Esophageal Cancer Progression By Regulating EZH2/β-catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXing Zhao,Qiuyang Chen,Yujie Cai,Danze Chen,Mingrong Bei,Hongyan Dong,Jianzhen XuPubMed 34234858
- Cell reports 25:3422-3434.e72018An Integrative Study of Protein-RNA Condensates Identifies Scaffolding RNAs and Reveals Players in Fragile X-Associated Tremor/Ataxia Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFernando Cid-Samper,Mariona Gelabert-Baldrich,Benjamin Lang,Nieves Lorenzo-Gotor,Riccardo Delli Ponti,Lies-Anne W F M Severijnen,Benedetta Bolognesi,Ellen Gelpi,Renate K Hukema,Teresa Botta-Orfila,Gian Gaetano TartagliaPubMed 30566867
- Life science alliance 1:e2018000702018Proteomics and neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannelore Hartmann et. al.PubMed 30456350
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TRA2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q13595
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Alternative names
Transformer-2 protein homolog alpha, TRA-2 alpha, TRA2-alpha, Transformer-2 protein homolog A, TRA2A
Recommended products
Rabbit Polyclonal TRA2A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRA2A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TRA2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q13595
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRA2A also known as transformer 2 alpha homolog is an RNA-binding protein involved in numerous cellular processes. This protein has a molecular weight of approximately 31 kDa. It is expressed in many tissues including the brain and testes. TRA2A binds to specific RNA sequences modulating splicing events and affecting the choice of splice sites. It belongs to the serine/arginine-rich protein family which plays key roles in the regulation of both alternative and constitutive splicing.
- Biological function summary
TRA2A influences gene expression and transcript variants by engaging in complex interactions. It acts as part of the spliceosome machinery which is a large ribonucleoprotein complex responsible for splicing pre-mRNA. TRA2A ensures precise removal of introns and ligation of exons producing different mRNA isoforms. These isoforms translate into functionally distinct proteins influencing cellular activity.
- Pathways
TRA2A participates in the mRNA processing pathway influencing genetic output by regulating splice site selection. TRA2A connects to the MAPK signaling pathway as it is known to interact with proteins like SAM68 which also affects mRNA splicing. This connectivity with other proteins highlights the regulatory network's complexity ensuring accurate gene expression profiles.
- Associated diseases and disorders
TRA2A has been implicated in cancer and neurodegenerative disorders. Altered expression and splicing activity of TRA2A contribute to oncogenesis as seen in breast cancer where abnormal splicing can drive tumor progression. TRA2A also impacts neurological conditions interplaying with proteins like tau in Alzheimer's disease where aberrant splicing plays a part in disease pathology. Understanding these links offers potential therapeutic angles for targeting TRA2A-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
This image is courtesy of an anonymous customer reviewWestern blot - Anti-TRA2A antibody (ab72625)
All lanes: Western blot - Anti-TRA2A antibody (ab72625) at 1/250 dilution
Lanes 1, 3 and 5: 293T whole cell lysate at 5 µg
Lanes 2, 4 and 6: Mouse ES (129Sv) whole cell lysate at 5 µg
SecondaryAll lanes: HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 27 kDa, 33 kDa, 85 kDa
Exposure time: 1min
Western blot - Anti-TRA2A antibody (ab72625)
All lanes: Western blot - Anti-TRA2A antibody (ab72625) at 1/500 dilution
Lane 1: Extracts from HUVEC cells at 5 µg
Lane 2: Extracts from HUVEC cells plus 5µg immunizing peptide at 5 µg
Predicted band size: 33 kDa
Observed band size: 27 kDa, 33 kDa, 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (ab72625)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab72625 at 1:50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (ab72625)
Immunofluorescence analysis of Hela cells using ab72625 at a 1:500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
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