AB223040

Anti-TRADD antibody

Name: Anti-TRADD antibody (ab223040) | Abcam
Brand: Abcam Limited
SKU: AB223040
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal TRADD antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRADD.

View Alternative Names

Tumor necrosis factor receptor type 1-associated DEATH domain protein, TNFR1-associated DEATH domain protein, TNFRSF1A-associated via death domain, TRADD

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody (AB223040)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody (AB223040)

U251 cells stained for TRADD (green) using ab223040 at 1/100 dilution in ICC/IF. Secondary : Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (AB223040)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (AB223040)

Paraffin-embedded human liver tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (AB223040)

Paraffin-embedded human tonsil tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-TRADD antibody (AB223040)

TRADD was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate with ab223040 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab223040 at 1/500 dilution. HRP-conjugated Protein G antibody was used as secondary antibody at 1/2000 dilution.

Lane 1 : Rabbit control IgG instead of ab223040 in K562 whole cell lysate.

Lane 2 : ab223040 IP in K562 whole cell lysate.

Lane 3 : K562 whole cell lysate 10 μg (Input).

All lanes:

Immunoprecipitation - Anti-TRADD antibody (ab223040)

Predicted band size: 34 kDa

Observed band size: 35 kDa

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Supplier Data

Western blot - Anti-TRADD antibody (AB223040)

All lanes:

Western blot - Anti-TRADD antibody (ab223040) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 2:

Rat kidney tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, IP, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TRADD.

Q15628

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRADD also known as TNFRSF1A-associated via death domain acts mechanically as an adaptor protein that interacts with tumor necrosis factor receptor 1 (TNFR1) and other signaling molecules. It has a mass of approximately 34 kDa. TRADD is expressed in various tissues including the spleen thymus and liver. It contributes to cellular responses by serving as an important component in TNF receptor signaling.

Biological function summary

The adaptor protein TRADD facilitates the transmission of death and survival signals from TNFR1. It forms part of a signaling complex that contributes to the activation of downstream pathways involved in apoptosis and inflammation. By interacting with other adaptor proteins like RIPK1 and FADD TRADD plays a role in assembling signaling complexes that govern cell fate decisions.

Pathways

TRADD is a pivotal player in both the TNF signaling and NF-kB pathways. In the TNF signaling pathway TRADD recruits RIPK1 and FADD leading to downstream activation of caspases and the apoptotic response. In the NF-kB pathway TRADD facilitates the activation of transcription factors that control genes involved in inflammatory responses. These interactions enable TRADD to mediate signals that influence cell survival proliferation and immune responses.

Dysfunction or overexpression of TRADD has connections to various conditions particularly cancer and autoimmune diseases. In cancer aberrant TNF signaling involving TRADD may promote survival and accumulation of malignant cells with overlapping involvement of NF-kB. Autoimmune diseases may arise when altered TRADD function affects inflammatory responses through interactions with TNFR1 and downstream NF-kB mediators. Understanding TRADD's role provides opportunities for therapeutic targeting in these health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD (PubMed : 23955153, PubMed : 7758105, PubMed : 8612133). Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B (PubMed : 7758105, PubMed : 8612133). The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12 : acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity).

See full target information TRADD

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and molecular physiology 324:L325-L334 PubMed36719084

2023

Hyperoxia impairs intraflagellar transport and causes dysregulated metabolism with resultant decreased cilia length.

Applications

Unspecified application

Species

Unspecified reactive species

Daniella Teape,Abigail Peterson,Nagib Ahsan,Kimberlyn Ellis,Nicholas Correia,Ryan Luo,Katy Hegarty,Hongwei Yao,Phyllis Dennery

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRADD antibody

Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody (AB223040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (AB223040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (AB223040)

Immunoprecipitation - Anti-TRADD antibody (AB223040)

Western blot - Anti-TRADD antibody (AB223040)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise