AB129279

Anti-TRAF1 antibody

Name: Anti-TRAF1 antibody (ab129279) | Abcam
Brand: Abcam Limited
SKU: AB129279
Price: 645 USD
Availability: InStock

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(5 Publications)

Goat Polyclonal TRAF1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TRAF1 aa 350 to C-terminus.

View Alternative Names

EBI6, TRAF1, TNF receptor-associated factor 1, Epstein-Barr virus-induced protein 6

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (AB129279)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (AB129279)

ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (AB129279)

ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human small intestine tissue by Immunohistochemistry.

Unknown

Western blot - Anti-TRAF1 antibody (AB129279)

All lanes:

Western blot - Anti-TRAF1 antibody (ab129279) at 0.5 µg/mL

All lanes:

U937 cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 46 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TRAF1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13077

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p></p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF1 or TNF Receptor Associated Factor 1 is a protein involved in signal transduction from various receptors including TNFR2 (tumor necrosis factor receptor 2). TRAF1 has a molecular mass of approximately 50 kDa. This protein is found mostly in the cytoplasm and functions as an adapter molecule. It helps to mediate signal transduction by interacting with receptor-associated signaling complexes. It does not have an intrinsic catalytic domain. TRAF1 expression is most prevalent in immune cells such as lymphocytes.

Biological function summary

TRAF1 plays a role in immune response regulation functioning as part of a larger TRAF protein complex that includes TRAF2 and TRAF3. This complex is significant for propagating signaling cascades important for immune system development and homeostasis. TRAF1 impacts the transcriptional activity that modulates inflammatory responses. It achieves this by interacting with other proteins to transmit signals from the cell surface to the nucleus subsequently affecting gene expression.

Pathways

TRAF1 is involved in the NF-κB and JNK signaling pathways which are important for immune cell activation and apoptosis regulation. In the NF-κB pathway TRAF1 works closely with TRAF2 facilitating the recruitment and activation of IKK (IκB kinase) which leads to the release and nuclear translocation of NF-κB. In the JNK pathway TRAF1 indirectly influences MAP kinase signaling cascades affecting cytokine production and cell death. These roles in signal transduction highlight TRAF1's function in maintaining immune cell function and integrity.

TRAF1 has connections to autoimmune diseases such as rheumatoid arthritis and certain cancers. In rheumatoid arthritis elevated TRAF1 expression correlates with inflammatory cell survival potentially exacerbating the disease. TRAF1 interaction with TNFR2 is significant in cancer aiding in tumor cell survival and proliferation. Its involvement with proteins like TRAF2 and cytokines like TNF-alpha highlights its impact on disease progression and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.

See full target information TRAF1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic translation 36:132-144 PubMed36185580

2022

Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ji,Yueyang Lu,Zhuoyi Ma,Ke Gan,Yan Liu,Yue Cheng,Junliang Xu,Shijia Liu,Yunke Guo,Shanhang Han,Zengyan Zhao,Hanmei Xu,Weiyan Qi

Experimental and therapeutic medicine 22:1311 PubMed34630665

2021

Blocking tumor necrosis factor-α delays progression of chronic obstructive pulmonary disease in rats through inhibiting MAPK signaling pathway and activating SOCS3/TRAF1.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Feng,Yan-Zi Yu,Qing-Hua Meng

Biochemical and biophysical research communications : PubMed31668814

2019

WITHDRAWN: ESM1-dependent mesenchymal transition enhances radioresistance of glioblastoma via transcriptional regulation of NFκB.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Wang,Jie Zuo,Alafate Wahafu,Hai Yu,Wanfu Xie,Hua Zhang,Maode Wang

Proceedings of the National Academy of Sciences of the United States of America 114:562-567 PubMed28031488

2016

CD74 is a novel transcription regulator.

Applications

Unspecified application

Species

Unspecified reactive species

Naama Gil-Yarom,Lihi Radomir,Lital Sever,Matthias P Kramer,Hadas Lewinsky,Chamutal Bornstein,Ronnie Blecher-Gonen,Zohar Barnett-Itzhaki,Vita Mirkin,Gilgi Friedlander,Lev Shvidel,Yair Herishanu,Elias J Lolis,Shirly Becker-Herman,Ido Amit,Idit Shachar

Nature communications 4:2852 PubMed24284943

2013

TRAF1 is a critical regulator of cerebral ischaemia-reperfusion injury and neuronal death.

Applications

IHC-Fr

Species

Mouse

Yan-Yun Lu,Zuo-Zhi Li,Ding-Sheng Jiang,Lang Wang,Yan Zhang,Ke Chen,Xiao-Fei Zhang,Yi Liu,Guo-Chang Fan,Yingjie Chen,Qinglin Yang,Yan Zhou,Xiao-Dong Zhang,De-Pei Liu,Hongliang Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (AB129279)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (AB129279)

Western blot - Anti-TRAF1 antibody (AB129279)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise