AB300075

Anti-TRAF1 antibody [EPR26204-112]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TRAF1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

EBI6, TRAF1, TNF receptor-associated factor 1, Epstein-Barr virus-induced protein 6

22 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 μ/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Cytoplasmic staining on human thymus (PMID : 11046039).The section was incubated with ab300075 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used. Cytoplasmic staining on Reed-Sternberg cells in human Hodgkin's lymphoma (PMID : 12502848). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on human thymus (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Human lymph node hyperplasia tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on human lymph node hyperplasia (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used. Cytoplasmic staining on Reed-Sternberg cells in human Hodgkin's lymphoma (PMID : 12502848). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Human lymph node hyperplasia tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on human lymph node hyperplasia (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

TRAF1 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg with ab300075 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300075 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg

Lane 2 : ab300075 in Raji whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300075 in Raji whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Supplier Data

Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

TRAF1 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg with ab300075 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300075 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg Lane 2 : ab300075 in Raji whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300075 in Raji whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

All lanes:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Exposure time: 10s

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labelling TRAF1 with ab300075 at 1/100 (5.64 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on mouse thymus medulla (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in mouse spleen (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

Supplier Data

Immunohistochemistry - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in mouse spleen (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in rat spleen (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on mouse thymus medulla (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in rat spleen (PMID : 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

Supplier Data

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

5% NFDM/TBST was used as blocking and iluting buffer.

Exposure time :

Lane 1-2 : 92 seconds
Lane3 : 3 minutes

Left-hand blot (lanes 1 & 2) was developed using a high sensitivity ECL substrate.
Lane 3 : Lysates were freshly made and used in WB test immediately to minimize protein degradation.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 25321474, 25996949).

All lanes:

Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

Daudi whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Supplier Data

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Daudi (PMID : 34589290)

Negative control : Jurkat (PMID : 11046039)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-TRAF1 antibody [EPR26204-112] (ab300075) staining at 1/1000 dilution.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] (<a href='/en-us/products/primary-antibodies/traf1-phospho-s146-antibody-epr25987-11-ab315154'>ab315154</a>) at 1/1000 dilution

Lane 1:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

Daudi (human burkitts lymphoma lymphoblast) whole cell lysate (untreated membrane) at 20 µg

Lane 3:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate (untreated membrane) at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate (untreated membrane) at 20 µg

Lane 5:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 6:

Daudi (human burkitts lymphoma lymphoblast) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 7:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 8:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 25321474, 25996949).

All lanes:

Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human lymphoma tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

true

Exposure time: 92s

Supplier Data

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

5% NFDM/TBST was used as blocking and diluting buffer. Exposure time : Lane 1-2 : 92 seconds Lane3 : 3 minutes Left-hand blot (lanes 1 & 2) was developed using a high sensitivity ECL substrate. Lane 3 : Lysates were freshly made and used in WB test immediately to minimize protein degradation. The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 25321474, 25996949).

All lanes:

Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate, at 20 µg

Lane 2:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

Daudi whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Supplier Data

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

5% NFDM/TBST was used as blocking and diluting buffer.

Lysates were freshly made and used in WB test immediately to minimize protein degradation.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 25321474, 25996949).
Negative control : HEK-293 (PMID : 12502848)

All lanes:

Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell fresh lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

5% NFDM/TBST was used as blocking and iluting buffer. Lysates were freshly made and used in WB test immediately to minimize protein degradation. The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 25321474, 25996949). Negative control : HEK-293 (PMID : 12502848)

All lanes:

Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell fresh lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

5% NFDM/TBST was used as blocking and diluting buffer.

This blot was developed using a high sensitivity ECL substrate.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 25321474, 25996949).

All lanes:

Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human lymphoma tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Exposure time: 92s

Supplier Data

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-TRAF1 antibody [EPR26204-112] (ab300075) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] (<a href='/en-us/products/primary-antibodies/traf1-phospho-s146-antibody-epr25987-11-ab315154'>ab315154</a>) at 1/1000 dilution

Lane 1:

Untreated Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

Raji treated with 100 nM Calycin A for 25 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Raji treated with 100 nM Calycin A for 25 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,15 kDa

false

Exposure time: 180s

Carrier free

Anti-TRAF1 antibody [EPR26204-112] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26204-112

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF1 or TNF Receptor Associated Factor 1 is a protein involved in signal transduction from various receptors including TNFR2 (tumor necrosis factor receptor 2). TRAF1 has a molecular mass of approximately 50 kDa. This protein is found mostly in the cytoplasm and functions as an adapter molecule. It helps to mediate signal transduction by interacting with receptor-associated signaling complexes. It does not have an intrinsic catalytic domain. TRAF1 expression is most prevalent in immune cells such as lymphocytes.

Biological function summary

TRAF1 plays a role in immune response regulation functioning as part of a larger TRAF protein complex that includes TRAF2 and TRAF3. This complex is significant for propagating signaling cascades important for immune system development and homeostasis. TRAF1 impacts the transcriptional activity that modulates inflammatory responses. It achieves this by interacting with other proteins to transmit signals from the cell surface to the nucleus subsequently affecting gene expression.

Pathways

TRAF1 is involved in the NF-κB and JNK signaling pathways which are important for immune cell activation and apoptosis regulation. In the NF-κB pathway TRAF1 works closely with TRAF2 facilitating the recruitment and activation of IKK (IκB kinase) which leads to the release and nuclear translocation of NF-κB. In the JNK pathway TRAF1 indirectly influences MAP kinase signaling cascades affecting cytokine production and cell death. These roles in signal transduction highlight TRAF1's function in maintaining immune cell function and integrity.

TRAF1 has connections to autoimmune diseases such as rheumatoid arthritis and certain cancers. In rheumatoid arthritis elevated TRAF1 expression correlates with inflammatory cell survival potentially exacerbating the disease. TRAF1 interaction with TNFR2 is significant in cancer aiding in tumor cell survival and proliferation. Its involvement with proteins like TRAF2 and cytokines like TNF-alpha highlights its impact on disease progression and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.

See full target information TRAF1

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Publications (1)

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Hereditas 162:44 PubMed40121492

2025

Activation of TRAF1 induced by USP7/SP1 exacerbates the severity of infantile pneumonia.

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Unspecified application

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Unspecified reactive species

Ying Liu,Yilun Ji,Yu Zhang,Zhengsi Li

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Anti-TRAF1 antibody [EPR26204-112]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Western blot - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

Related conjugates and formulations (1)

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Publications (1)

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