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Rabbit Monoclonal TRAF1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Rat, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-PIP
Human
Tested
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Tested
Expected
Rat
Not recommended
Not recommended
Not recommended
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.

Alternative names

Recommended products

Rabbit Monoclonal TRAF1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26204-112
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRAF1 or TNF Receptor Associated Factor 1 is a protein involved in signal transduction from various receptors including TNFR2 (tumor necrosis factor receptor 2). TRAF1 has a molecular mass of approximately 50 kDa. This protein is found mostly in the cytoplasm and functions as an adapter molecule. It helps to mediate signal transduction by interacting with receptor-associated signaling complexes. It does not have an intrinsic catalytic domain. TRAF1 expression is most prevalent in immune cells such as lymphocytes.

Biological function summary

TRAF1 plays a role in immune response regulation functioning as part of a larger TRAF protein complex that includes TRAF2 and TRAF3. This complex is significant for propagating signaling cascades important for immune system development and homeostasis. TRAF1 impacts the transcriptional activity that modulates inflammatory responses. It achieves this by interacting with other proteins to transmit signals from the cell surface to the nucleus subsequently affecting gene expression.

Pathways

TRAF1 is involved in the NF-κB and JNK signaling pathways which are important for immune cell activation and apoptosis regulation. In the NF-κB pathway TRAF1 works closely with TRAF2 facilitating the recruitment and activation of IKK (IκB kinase) which leads to the release and nuclear translocation of NF-κB. In the JNK pathway TRAF1 indirectly influences MAP kinase signaling cascades affecting cytokine production and cell death. These roles in signal transduction highlight TRAF1's function in maintaining immune cell function and integrity.

Associated diseases and disorders

TRAF1 has connections to autoimmune diseases such as rheumatoid arthritis and certain cancers. In rheumatoid arthritis elevated TRAF1 expression correlates with inflammatory cell survival potentially exacerbating the disease. TRAF1 interaction with TNFR2 is significant in cancer aiding in tumor cell survival and proliferation. Its involvement with proteins like TRAF2 and cytokines like TNF-alpha highlights its impact on disease progression and potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

22 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used. Cytoplasmic staining on Reed-Sternberg cells in human Hodgkin's lymphoma (PMID: 12502848). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in rat spleen (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in mouse spleen (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labelling TRAF1 with ab300075 at 1/100 (5.64 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on mouse thymus medulla (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 μ/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Cytoplasmic staining on human thymus (PMID: 11046039).The section was incubated with ab300075 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Human lymph node hyperplasia tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 μg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on human lymph node hyperplasia (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    5% NFDM/TBST was used as blocking and diluting buffer.

    Lysates were freshly made and used in WB test immediately to minimize protein degradation.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949).
    Negative control: HEK-293 (PMID: 12502848)

    All lanes: Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell fresh lysate at 20 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell fresh lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    TRAF1 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg with ab300075 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300075 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg

    Lane 2: ab300075 in Raji whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300075 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    5% NFDM/TBST was used as blocking and iluting buffer.

    Exposure time:

    Lane 1-2: 92 seconds
    Lane3: 3 minutes

    Left-hand blot (lanes 1 & 2) was developed using a high sensitivity ECL substrate.
    Lane 3: Lysates were freshly made and used in WB test immediately to minimize protein degradation.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949).

    All lanes: Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

    Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 2: Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 3: Daudi whole cell fresh lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    5% NFDM/TBST was used as blocking and diluting buffer.

    This blot was developed using a high sensitivity ECL substrate.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949).

    All lanes: Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 20 µg

    Lane 2: Human lymphoma tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

    Exposure time: 92s

  • Immunohistochemistry - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in mouse spleen (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949). 

    All lanes: Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 20 µg

    Lane 2: Human lymphoma tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

    Exposure time: 92s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on megakaryocytes in rat spleen (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Daudi (PMID: 34589290)

    Negative control: Jurkat (PMID: 11046039)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-TRAF1 antibody [EPR26204-112] (ab300075) staining at 1/1000 dilution.

    This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] (Anti-TRAF1 (phospho S146) antibody [EPR25987-11] ab315154) at 1/1000 dilution

    Lane 1: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (untreated membrane) at 20 µg

    Lane 2: Daudi (human burkitts lymphoma lymphoblast) whole cell lysate (untreated membrane) at 20 µg

    Lane 3: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate (untreated membrane) at 20 µg

    Lane 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate (untreated membrane) at 20 µg

    Lane 5: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 6: Daudi (human burkitts lymphoma lymphoblast) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 7: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 8: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    In Western blot, Anti-TRAF1 antibody [EPR26204-112] (ab300075) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] (Anti-TRAF1 (phospho S146) antibody [EPR25987-11] ab315154) at 1/1000 dilution

    Lane 1: Untreated Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (untreated membrane) at 20 µg

    Lane 2: Raji treated with 100 nM Calycin A for 25 minutes whole cell lysate (untreated membrane) at 20 µg

    Lane 3: Untreated Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 4: Raji treated with 100 nM Calycin A for 25 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50 kDa, 15 kDa

    Exposure time: 180s

  • Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    5% NFDM/TBST was used as blocking and iluting buffer. 

    Lysates were freshly made and used in WB test immediately to minimize protein degradation.

    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949).

    Negative control: HEK-293 (PMID: 12502848)

    All lanes: Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell fresh lysate at 20 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell fresh lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

    Exposure time: 15s

  • Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    5% NFDM/TBST was used as blocking and diluting buffer. 

    Exposure time: 

    Lane 1-2: 92 seconds
    Lane3: 3 minutes

    Left-hand blot (lanes 1 & 2) was developed using a high sensitivity ECL substrate.
    Lane 3: Lysates were freshly made and used in WB test immediately to minimize protein degradation.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25321474, 25996949).

    All lanes: Western blot - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

    Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate, at 20 µg

    Lane 2: Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 3: Daudi whole cell fresh lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used. Cytoplasmic staining on Reed-Sternberg cells in human Hodgkin's lymphoma (PMID: 12502848). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Human lymph node hyperplasia tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on human lymph node hyperplasia (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labelling TRAF1 with ab300075 at 1/1000 (0.564 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on human thymus (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labelling TRAF1 with ab300075 at 1/100 (5.64 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Cytoplasmic staining on mouse thymus medulla (PMID: 11046039). The section was incubated with ab300075 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (ab300075), expandable thumbnail

    Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (ab300075)

    TRAF1 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg with ab300075 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300075 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg

    Lane 2: ab300075 in Raji whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300075 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-TRAF1 antibody [EPR26204-112] (ab300075) at 1/1000 dilution

    Lanes 1 - 2: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

    Exposure time: 10s

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