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AB315155

Anti-TRAF1 (phospho S146) antibody [EPR25987-11] - BSA and Azide free

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Rabbit Recombinant Monoclonal TRAF1 phospho S146 antibody. Carrier free. Suitable for WB, Dot and reacts with Human, Synthetic peptide - Human samples.

View Alternative Names

EBI6, TRAF1, TNF receptor-associated factor 1, Epstein-Barr virus-induced protein 6

3 Images
Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] - BSA and Azide free (AB315155)
  • WB

Supplier Data

Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] - BSA and Azide free (AB315155)

This data was developed using ab315154, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-TRAF1 antibody [EPR26204-112] (ab300075) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] (<a href='/en-us/products/primary-antibodies/traf1-phospho-s146-antibody-epr25987-11-ab315154'>ab315154</a>) at 1/1000 dilution

Lane 1:

Untreated Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

Raji treated with 100 nM Calycin A for 25 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Raji treated with 100 nM Calycin A for 25 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,15 kDa

false

Exposure time: 180s

Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] - BSA and Azide free (AB315155)
  • WB

Supplier Data

Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] - BSA and Azide free (AB315155)

This data was developed using ab315154, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Daudi (PMID : 34589290)

Negative control : Jurkat (PMID : 11046039)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-TRAF1 antibody [EPR26204-112] (ab300075) staining at 1/1000 dilution.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] (<a href='/en-us/products/primary-antibodies/traf1-phospho-s146-antibody-epr25987-11-ab315154'>ab315154</a>) at 1/1000 dilution

Lane 1:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

Daudi (human burkitts lymphoma lymphoblast) whole cell lysate (untreated membrane) at 20 µg

Lane 3:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate (untreated membrane) at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate (untreated membrane) at 20 µg

Lane 5:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 6:

Daudi (human burkitts lymphoma lymphoblast) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 7:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 8:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 180s

Dot Blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] - BSA and Azide free (AB315155)
  • Dot

Supplier Data

Dot Blot - Anti-TRAF1 (phospho S146) antibody [EPR25987-11] - BSA and Azide free (AB315155)

This data was developed using ab315154, the same antibody clone in a different buffer formulation.

Dot blot analysis of TRAF1 (phospho S146) using ab315154 at 1 : 1000 (0.471 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

  • Unconjugated

    Anti-TRAF1 (phospho S146) antibody [EPR25987-11]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25987-11

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab315155 is the carrier-free version of ab315154.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF1 or TNF Receptor Associated Factor 1 is a protein involved in signal transduction from various receptors including TNFR2 (tumor necrosis factor receptor 2). TRAF1 has a molecular mass of approximately 50 kDa. This protein is found mostly in the cytoplasm and functions as an adapter molecule. It helps to mediate signal transduction by interacting with receptor-associated signaling complexes. It does not have an intrinsic catalytic domain. TRAF1 expression is most prevalent in immune cells such as lymphocytes.
Biological function summary

TRAF1 plays a role in immune response regulation functioning as part of a larger TRAF protein complex that includes TRAF2 and TRAF3. This complex is significant for propagating signaling cascades important for immune system development and homeostasis. TRAF1 impacts the transcriptional activity that modulates inflammatory responses. It achieves this by interacting with other proteins to transmit signals from the cell surface to the nucleus subsequently affecting gene expression.

Pathways

TRAF1 is involved in the NF-κB and JNK signaling pathways which are important for immune cell activation and apoptosis regulation. In the NF-κB pathway TRAF1 works closely with TRAF2 facilitating the recruitment and activation of IKK (IκB kinase) which leads to the release and nuclear translocation of NF-κB. In the JNK pathway TRAF1 indirectly influences MAP kinase signaling cascades affecting cytokine production and cell death. These roles in signal transduction highlight TRAF1's function in maintaining immune cell function and integrity.

TRAF1 has connections to autoimmune diseases such as rheumatoid arthritis and certain cancers. In rheumatoid arthritis elevated TRAF1 expression correlates with inflammatory cell survival potentially exacerbating the disease. TRAF1 interaction with TNFR2 is significant in cancer aiding in tumor cell survival and proliferation. Its involvement with proteins like TRAF2 and cytokines like TNF-alpha highlights its impact on disease progression and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
See full target information TRAF1 phospho S146
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com