AB244317

Anti-TRAF2 antibody

Name: Anti-TRAF2 antibody (ab244317) | Abcam
Brand: Abcam Limited
SKU: AB244317
Price: 630 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal TRAF2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRAF2 aa 1-200.

View Alternative Names

TRAP3, TRAF2, TNF receptor-associated factor 2, E3 ubiquitin-protein ligase TRAF2, RING-type E3 ubiquitin transferase TRAF2, Tumor necrosis factor type 2 receptor-associated protein 3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody (AB244317)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody (AB244317)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TRAF2 (green) using ab244317 at 4 μg/ml in ICC/IF.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody (AB244317)

Formalin-fixed, paraffin-embedded human testis tissue stained for TRAF2 using ab244317 at 1/20 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-TRAF2 antibody (AB244317)

All lanes:

Western blot - Anti-TRAF2 antibody (ab244317) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Predicted band size: 55 kDa

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Western blot - Anti-TRAF2 antibody (AB244317)

All lanes:

Western blot - Anti-TRAF2 antibody (ab244317) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat Wistar cell line) cell lysate

Predicted band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TRAF2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q12933

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF2 known also as TNF Receptor-Associated Factor 2 is an adapter protein weighing approximately 56 kDa. It is expressed in many tissues such as lymph nodes spleen and thymus. This protein acts as a signal transducer playing an integral role in the activation of NF-kB and MAPK signaling pathways. TRAF2 interacts with TNF receptors and other proteins using its RING finger and zinc finger domains for ubiquitination processes.

Biological function summary

TRAF2 modulates immune responses by regulating cell survival proliferation and apoptosis. It often forms complexes with other TRAF family proteins to mediate signal transmission. These complexes control the balance between pro-apoptotic and anti-apoptotic signals impacting the regulation of immune and inflammatory responses. Its interactions are necessary for the proper function of NF-kB ensuring balanced immune activities.

Pathways

TRAF2 is a critical component within both NF-kB and JNK signaling pathways. In the NF-kB pathway TRAF2 intersects with proteins like IKK and NEMO facilitating the transcription of genes involved in immune and inflammatory responses. In JNK signaling TRAF2 influences cellular responses through regulation of apoptotic signals. By linking these pathways TRAF2 ensures a coordinated cellular response to extracellular stimuli.

TRAF2’s dysregulation is linked with autoimmune diseases and certain types of cancer. Overactivity can contribute to chronic inflammation highlighting its role in autoimmune disorders like rheumatoid arthritis. In cancer TRAF2’s involvement with TNF receptors can lead to uncontrolled cell proliferation. Proteins like cIAP1 and cIAP2 are often related to TRAF2 in these contexts influencing the progression and modulation of disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis (PubMed : 22212761). Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.

See full target information TRAF2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 16:5829-5845 PubMed39544779

2024

Zhi Zhu Ma Ren pill relieves constipation in mice through endoplasmic reticulum stress-mediated apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wen,Yu Zhan,Jun Li,Ling Xu,Chengzi Huang,Rong Wu,Xuegui Tang

Frontiers in immunology 14:1160490 PubMed37359541

2023

-GlcNAcylation of RIPK1 rescues red blood cells from necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junghwa Seo,Yeolhoe Kim,Suena Ji,Han Byeol Kim,Hyeryeon Jung,Eugene C Yi,Yong-Ho Lee,Injae Shin,Won Ho Yang,Jin Won Cho

General physiology and biophysics 41:151-158 PubMed35416178

2022

TRAF3 plays a role in lupus nephritis by regulating Th17 cell and Treg cell balance as well as NF-κB signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Qian Sun,Wen-Si Liu,Hui-Ming Zhang,Chun-Yan Xuan,Yue Wang,Bing-Bing Fu

Journal of cellular and molecular medicine 25:11039-11052 PubMed34791807

2021

Pharmacological inhibition of Carbonic Anhydrase IX and XII to enhance targeting of acute myeloid leukaemia cells under hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Fangli Chen,Emilia Licarete,Xue Wu,Daniela Petrusca,Callista Maguire,Max Jacobsen,Austyn Colter,George E Sandusky,Magdalena Czader,Maegan L Capitano,James P Ropa,H Scott Boswell,Fabrizio Carta,Claudiu T Supuran,Brian Parkin,Melissa L Fishel,Heiko Konig

Cellular signalling 73:109690 PubMed32553549

2020

Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-κB/iNOS/NO-dependent cardiomyocyte contractile depression and death.

Applications

Unspecified application

Species

Unspecified reactive species

Tadashi Yoshida,Nitin A Das,Andrea J Carpenter,Reza Izadpanah,Senthil A Kumar,Sandeep Gautam,Shawn B Bender,Ulrich Siebenlist,Bysani Chandrasekar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAF2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody (AB244317)

Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody (AB244317)

Western blot - Anti-TRAF2 antibody (AB244317)

Western blot - Anti-TRAF2 antibody (AB244317)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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