Rabbit Polyclonal TRAF3 antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TRAF3 aa 100-200 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Hepatology (Baltimore, Md.) 77:395-4102022TNF receptor-related factor 3 inactivation promotes the development of intrahepatic cholangiocarcinoma through NF-κB-inducing kinase-mediated hepatocyte transdifferentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuto Shiode,Takahiro Kodama,Satoshi Shigeno,Kazuhiro Murai,Satoshi Tanaka,Justin Y Newberg,Jumpei Kondo,Shogo Kobayashi,Ryoko Yamada,Hayato Hikita,Ryotaro Sakamori,Hiroshi Suemizu,Tomohide Tatsumi,Hidetoshi Eguchi,Nancy A Jenkins,Neal G Copeland,Tetsuo TakeharaPubMed 34995376
- The American journal of pathology 190:1701-17122020Tumor Necrosis Factor Receptor Associated Factor 3 Modulates Cartilage Degradation through Suppression of Interleukin 17 Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoli Hu,Ning Zhang,Jiao Li,Jing Wang,Wen Wu,Jing Li,Wenxue Tong,Xiaoying Zhao,Liming Dai,Xiaoling ZhangPubMed 32416098
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TRAF3 aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13114
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus controls a lot of biological processes in both immune and non-immune cell types (PubMed:33148796, PubMed:33608556). In TLR and RLR signaling pathways, acts as an E3 ubiquitin ligase promoting the synthesis of 'Lys-63'-linked polyubiquitin chains on several substrates such as ASC that lead to the activation of the type I interferon response or the inflammasome (PubMed:25847972, PubMed:27980081). Following the activation of certain TLRs such as TLR4, acts as a negative NF-kappa-B regulator, possibly to avoid unregulated inflammatory response, and its degradation via 'Lys-48'-linked polyubiquitination is required for MAPK activation and production of inflammatory cytokines. Alternatively, when TLR4 orchestrates bacterial expulsion, TRAF3 undergoes 'Lys-33'-linked polyubiquitination and subsequently binds to RALGDS, mobilizing the exocyst complex to rapidly expel intracellular bacteria back for clearance (PubMed:27438768). Acts also as a constitutive negative regulator of the alternative NF-kappa-B pathway, which controls B-cell survival and lymphoid organ development. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
Alternative names
CAP-1, CRAF1, TRAFAMN, TRAF3, TNF receptor-associated factor 3, CD40 receptor-associated factor 1, CD40-binding protein, LMP1-associated protein 1, RING-type E3 ubiquitin transferase TRAF3, CD40BP, LAP1
Recommended products
Rabbit Polyclonal TRAF3 antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TRAF3 aa 100-200 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TRAF3 aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13114
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAF3 also known as TNF receptor-associated factor 3 weighs approximately 63 kDa. This adaptor protein is expressed in many tissues including lymphoid organs and the spleen. Mechanically TRAF3 interacts with the tumor necrosis factor receptor (TNFR) superfamily serving as a pivotal mediator in transducing signals. The protein engages directly with cytoplasmic domains of receptors relaying information downstream to influence cellular responses.
- Biological function summary
The protein TRAF3 influences immune response regulation and lymphocyte activation by being part of signaling complexes. It binds with other TRAF family members forming multimeric structures for modulating NF-kB and MAPK pathways. TRAF3 plays a role in both adaptive and innate immune mechanisms controlling cell survival signals and apoptosis. Its activity impacts B-cell and T-cell functions further emphasizing its role in immune cell dynamics.
- Pathways
TRAF3 significantly impacts NF-kB activation and MAP kinase signaling. It cooperates with other proteins like TRAF2 and NIK to modulate these pathways. The NF-kB pathway important for immune and inflammatory responses involves TRAF3 in negatively regulating this pathway by targeting NIK for degradation. Similarly in the MAPK signaling TRAF3 influences response to environmental stresses.
- Associated diseases and disorders
TRAF3 connects to conditions such as autoimmune diseases and lymphoma. In autoimmune diseases the improper function of TRAF3 affects immune tolerance leading to abnormal B-cell activity. In lymphomas TRAF3 mutations can result in loss of function contributing to the development of B-cell malignancies. Interactions with proteins like TRAF2 in these disorders illustrate its significance in pathology and potential targeting in therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF3 antibody (ab217033)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human gastric carcinoma tissue labeling TRAF3 with ab217033 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF3 antibody (ab217033)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse lymphoma tissue labeling TRAF3 with ab217033 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
Flow Cytometry (Intracellular) - Anti-TRAF3 antibody (ab217033)
Intracellular flow cytometric analysis of Jurkat cellslabeling TRAF3 with ab217033 at 0.2μg for 30 minutes followed by incubation with a PE Conjugated secondary for 30 minutes (green), compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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