Rabbit Recombinant Monoclonal TRAF3 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Images
![Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357), expandable thumbnail](https://content.abcam.com/products/images/traf3-antibody-epr22992-93-ab239357--western-blot-img16185.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357)")
![Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357), expandable thumbnail](https://content.abcam.com/products/images/traf3-antibody-epr22992-93-ab239357--western-blot-img113551.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357)")
Publications
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- Life science alliance 7:2023FAT10 is phosphorylated by IKKβ to inhibit the antiviral type-I interferon response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKritika Saxena,Nicola Domenico Roverato,Melody Reithmann,Mei Min Mah,Richard Schregle,Gunter Schmidtke,Ivan Silbern,Henning Urlaub,Annette AichemPubMed 37940187
- Frontiers in pharmacology 13:9059662022Curcumenol Mitigates the Inflammation and Ameliorates the Catabolism Status of the Intervertebral Discs Inhibiting the TNFα/NFκB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Yang,Baixing Li,Haijun Tian,Xiaofei Cheng,Tangjun Zhou,Jie ZhaoPubMed 35795557
- Liver international : official journal of the International Association for the Study of the Liver 42:1676-16912022Hepatic exosomes with declined MiR-27b-3p trigger RIG-I/TBK1 signal pathway in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie You,Wenyu Wu,Mengxin Lu,Yanghao Xie,Rui Miao,Misi Gu,Dong Xi,Weiming Yan,Di Wu,Xiaojing Wang,Tao Chen,Qin Ning,Meifang HanPubMed 35460174
- Molecular therapy. Nucleic acids 24:711-7272021HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi Zhang,Huiqing Wen,Bangjian Peng,Jun Weng,Fanhong ZengPubMed 33996254
- OncoTargets and therapy 13:8783-87942020RIG-I Promotes Cell Death in Hepatocellular Carcinoma by Inducing M1 Polarization of Perineal Macrophages Through the RIG-I/MAVS/NF-κB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBei Zhou et. al.PubMed 32982277
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus controls a lot of biological processes in both immune and non-immune cell types (PubMed:33148796, PubMed:33608556). In TLR and RLR signaling pathways, acts as an E3 ubiquitin ligase promoting the synthesis of 'Lys-63'-linked polyubiquitin chains on several substrates such as ASC that lead to the activation of the type I interferon response or the inflammasome (PubMed:25847972, PubMed:27980081). Following the activation of certain TLRs such as TLR4, acts as a negative NF-kappa-B regulator, possibly to avoid unregulated inflammatory response, and its degradation via 'Lys-48'-linked polyubiquitination is required for MAPK activation and production of inflammatory cytokines. Alternatively, when TLR4 orchestrates bacterial expulsion, TRAF3 undergoes 'Lys-33'-linked polyubiquitination and subsequently binds to RALGDS, mobilizing the exocyst complex to rapidly expel intracellular bacteria back for clearance (PubMed:27438768). Acts also as a constitutive negative regulator of the alternative NF-kappa-B pathway, which controls B-cell survival and lymphoid organ development. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
Alternative names
CAP-1, CRAF1, TRAFAMN, TRAF3, TNF receptor-associated factor 3, CD40 receptor-associated factor 1, CD40-binding protein, LMP1-associated protein 1, RING-type E3 ubiquitin transferase TRAF3, CD40BP, LAP1
Recommended products
Rabbit Recombinant Monoclonal TRAF3 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22992-93
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAF3 also known as TNF receptor-associated factor 3 weighs approximately 63 kDa. This adaptor protein is expressed in many tissues including lymphoid organs and the spleen. Mechanically TRAF3 interacts with the tumor necrosis factor receptor (TNFR) superfamily serving as a pivotal mediator in transducing signals. The protein engages directly with cytoplasmic domains of receptors relaying information downstream to influence cellular responses.
- Biological function summary
The protein TRAF3 influences immune response regulation and lymphocyte activation by being part of signaling complexes. It binds with other TRAF family members forming multimeric structures for modulating NF-kB and MAPK pathways. TRAF3 plays a role in both adaptive and innate immune mechanisms controlling cell survival signals and apoptosis. Its activity impacts B-cell and T-cell functions further emphasizing its role in immune cell dynamics.
- Pathways
TRAF3 significantly impacts NF-kB activation and MAP kinase signaling. It cooperates with other proteins like TRAF2 and NIK to modulate these pathways. The NF-kB pathway important for immune and inflammatory responses involves TRAF3 in negatively regulating this pathway by targeting NIK for degradation. Similarly in the MAPK signaling TRAF3 influences response to environmental stresses.
- Associated diseases and disorders
TRAF3 connects to conditions such as autoimmune diseases and lymphoma. In autoimmune diseases the improper function of TRAF3 affects immune tolerance leading to abnormal B-cell activity. In lymphomas TRAF3 mutations can result in loss of function contributing to the development of B-cell malignancies. Interactions with proteins like TRAF2 in these disorders illustrate its significance in pathology and potential targeting in therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357), expandable thumbnail](https://content.abcam.com/products/images/traf3-antibody-epr22992-93-ab239357--western-blot-img16185.jpg "Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)")
Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab239357 was shown to specifically react with TRAF3 in wild-type HAP1 cells as signal was lost in TRAF3 knockout cells. Wild-type and TRAF3 knockout samples were subjected to SDS-PAGE. ab239357 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. Exposure time: 37 seconds.
All lanes: Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution
Lane 1: Wild type HAP1 whole cell lysate at 40 µg
Lane 2: TRAF3 knockout HAP1 whole cell lysate at 40 µg
Lane 3: Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 40 µg
Lane 4: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
![Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357), expandable thumbnail](https://content.abcam.com/products/images/traf3-antibody-epr22992-93-ab239357--western-blot-img113551.jpg "Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)")
Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 128 seconds; Lane 5: 3 minutes; Lane 6: 125 seconds; Lane 7: 8 seconds.
All lanes: Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Lane 5: Mouse brain tissue lysate at 10 µg
Lane 6: Rat spleen tissue lysate at 10 µg
Lane 7: Rat brain tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
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