AB239357

Anti-TRAF3 antibody [EPR22992-93]

RabMAb
Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(11 Publications)

Rabbit Recombinant Monoclonal TRAF3 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

View Alternative Names

CAP-1, CRAF1, TRAFAMN, TRAF3, TNF receptor-associated factor 3, CD40 receptor-associated factor 1, CD40-binding protein, LMP1-associated protein 1, RING-type E3 ubiquitin transferase TRAF3, CD40BP, LAP1

2 Images

Lab

Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ab239357 was shown to specifically react with TRAF3 in wild-type HAP1 cells as signal was lost in TRAF3 knockout cells. Wild-type and TRAF3 knockout samples were subjected to SDS-PAGE. ab239357 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. Exposure time : 37 seconds.

All lanes:

Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution

Lane 1:

Wild type HAP1 whole cell lysate at 40 µg

Lane 2:

TRAF3 knockout HAP1 whole cell lysate at 40 µg

Lane 3:

Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 40 µg

Lane 4:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Lab

Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1-4 : 128 seconds; Lane 5 : 3 minutes; Lane 6 : 125 seconds; Lane 7 : 8 seconds.

All lanes:

Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Lane 5:

Mouse brain tissue lysate at 10 µg

Lane 6:

Rat spleen tissue lysate at 10 µg

Lane 7:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Carrier free

Anti-TRAF3 antibody [EPR22992-93] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22992-93

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF3 also known as TNF receptor-associated factor 3 weighs approximately 63 kDa. This adaptor protein is expressed in many tissues including lymphoid organs and the spleen. Mechanically TRAF3 interacts with the tumor necrosis factor receptor (TNFR) superfamily serving as a pivotal mediator in transducing signals. The protein engages directly with cytoplasmic domains of receptors relaying information downstream to influence cellular responses.

Biological function summary

The protein TRAF3 influences immune response regulation and lymphocyte activation by being part of signaling complexes. It binds with other TRAF family members forming multimeric structures for modulating NF-kB and MAPK pathways. TRAF3 plays a role in both adaptive and innate immune mechanisms controlling cell survival signals and apoptosis. Its activity impacts B-cell and T-cell functions further emphasizing its role in immune cell dynamics.

Pathways

TRAF3 significantly impacts NF-kB activation and MAP kinase signaling. It cooperates with other proteins like TRAF2 and NIK to modulate these pathways. The NF-kB pathway important for immune and inflammatory responses involves TRAF3 in negatively regulating this pathway by targeting NIK for degradation. Similarly in the MAPK signaling TRAF3 influences response to environmental stresses.

TRAF3 connects to conditions such as autoimmune diseases and lymphoma. In autoimmune diseases the improper function of TRAF3 affects immune tolerance leading to abnormal B-cell activity. In lymphomas TRAF3 mutations can result in loss of function contributing to the development of B-cell malignancies. Interactions with proteins like TRAF2 in these disorders illustrate its significance in pathology and potential targeting in therapeutic interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus controls a lot of biological processes in both immune and non-immune cell types (PubMed : 33148796, PubMed : 33608556). In TLR and RLR signaling pathways, acts as an E3 ubiquitin ligase promoting the synthesis of 'Lys-63'-linked polyubiquitin chains on several substrates such as ASC that lead to the activation of the type I interferon response or the inflammasome (PubMed : 25847972, PubMed : 27980081). Following the activation of certain TLRs such as TLR4, acts as a negative NF-kappa-B regulator, possibly to avoid unregulated inflammatory response, and its degradation via 'Lys-48'-linked polyubiquitination is required for MAPK activation and production of inflammatory cytokines. Alternatively, when TLR4 orchestrates bacterial expulsion, TRAF3 undergoes 'Lys-33'-linked polyubiquitination and subsequently binds to RALGDS, mobilizing the exocyst complex to rapidly expel intracellular bacteria back for clearance (PubMed : 27438768). Also acts as a constitutive negative regulator of the alternative NF-kappa-B pathway, which controls B-cell survival and lymphoid organ development. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.

See full target information TRAF3

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:3917-3936 PubMed40615576

2025

NAT10-mediated acetylation of NIK mRNA in B cells promotes IgA production.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Jun Jiang,Xin-Tao Mao,Wen-Ping Li,Nicole Jin,Yu Wang,Guiping Guan,Jin Jin,Yi-Yuan Li

Communications biology 8:457 PubMed40102597

2025

Peli1, regulated by mA modification, suppresses NLRP3 inflammasome activation in atherosclerosis by inhibiting YB-1.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Liu,Lu Yan,Tao Wu,Qinghua Wu,Ben Ke,Wen Shen

Scientific reports 14:12728 PubMed38830934

2024

SPA inhibits hBMSC osteogenic differentiation and M1 macrophage polarization by suppressing SETD2 in acute suppurative osteomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Zhu,Feng Chen,Hongjia Qiang,Han Qi

Life science alliance 7: PubMed37940187

2023

FAT10 is phosphorylated by IKKβ to inhibit the antiviral type-I interferon response.

Applications

Unspecified application

Species

Unspecified reactive species

Kritika Saxena,Nicola Domenico Roverato,Melody Reithmann,Mei Min Mah,Richard Schregle,Gunter Schmidtke,Ivan Silbern,Henning Urlaub,Annette Aichem

International heart journal 64:263-273 PubMed37005320

2023

CircUSP39/miR-362-3p/TRAF3 Axis Mediates Hypoxia/Reoxygenation-Induced Cardiomyocyte Oxidative Stress, Inflammation, and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Xuan Wang,Mingying Cao,Lingli Zhang,Jingna Lin

Cell reports 40:111225 PubMed35977521

2022

The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Milton Pereira,Danielle F Durso,Clare E Bryant,Evelyn A Kurt-Jones,Neal Silverman,Douglas T Golenbock,Ricardo T Gazzinelli

Frontiers in pharmacology 13:905966 PubMed35795557

2022

Curcumenol Mitigates the Inflammation and Ameliorates the Catabolism Status of the Intervertebral Discs Inhibiting the TNFα/NFκB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Yang,Baixing Li,Haijun Tian,Xiaofei Cheng,Tangjun Zhou,Jie Zhao

Liver international : official journal of the International Association for the Study of the Liver 42:1676-1691 PubMed35460174

2022

Hepatic exosomes with declined MiR-27b-3p trigger RIG-I/TBK1 signal pathway in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Jie You,Wenyu Wu,Mengxin Lu,Yanghao Xie,Rui Miao,Misi Gu,Dong Xi,Weiming Yan,Di Wu,Xiaojing Wang,Tao Chen,Qin Ning,Meifang Han

Cell death and differentiation 29:206-217 PubMed34465890

2021

Mechanosensitive turnover of phosphoribosyl pyrophosphate synthetases regulates nucleotide metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Jichun Shao,Zhijun Zeng,Yumin He,Can Tang,Su Hwan Park,Jong-Ho Lee,Rui Liu

Molecular therapy. Nucleic acids 24:711-727 PubMed33996254

2021

HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Zhang,Huiqing Wen,Bangjian Peng,Jun Weng,Fanhong Zeng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAF3 antibody [EPR22992-93]

Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357)

Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise