AB108991

Anti-TRAF4 antibody [EPR1729]

Name: Anti-TRAF4 antibody [EPR1729] (ab108991) | Abcam
Brand: Abcam Limited
SKU: AB108991
Price: 570 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal TRAF4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CART1, MLN62, RNF83, TRAF4, TNF receptor-associated factor 4, Cysteine-rich domain associated with RING and Traf domains protein 1, Metastatic lymph node gene 62 protein, RING finger protein 83, MLN 62

2 Images

Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] (AB108991)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] (AB108991)

Overlay histogram showing HeLa cells stained with ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108991 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Western blot - Anti-TRAF4 antibody [EPR1729] (AB108991)

All lanes:

Western blot - Anti-TRAF4 antibody [EPR1729] (ab108991) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

SKBR-3 cell lysate at 10 µg

Predicted band size: 54 kDa

false

Carrier free

Anti-TRAF4 antibody [EPR1729] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1729

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF4 also known as Tumor Necrosis Factor Receptor-Associated Factor 4 is a scaffold protein with a molecular weight of approximately 55 kDa. TRAF4 interacts with a variety of intracellular signaling molecules influencing signal transduction. It is expressed prominently in epithelial cells and the respiratory tract reflecting its involvement in tissue homeostasis and cell communication. The protein contains a conserved RING finger domain a novel zinc finger TRAF-type domain and a coiled-coil region which are essential for its function in protein-protein interactions.

Biological function summary

TRAF4 participates in the regulation of immune responses and development of epithelial tissues. This protein modulates signaling pathways by promoting or inhibiting the activation of transcription factors including NF-κB and AP-1 which are critical for the immune system's function. TRAF4 associates with various adapter proteins forming signaling complexes that influence cellular outcomes during immune responses. It also plays a role in the development and integrity of the respiratory epithelium highlighting its importance in maintaining epithelial barrier functions.

Pathways

TRAF4 integrates into the TNF receptor signaling pathway and the TGF-beta signaling pathway. In the TNF receptor pathway TRAF4 interacts with other TRAF family members mediating responses to inflammatory cytokines and regulating apoptosis. Within the TGF-beta pathway TRAF4 modulates signal transduction involving Smad proteins impacting cellular growth and differentiation. The protein's role within these pathways highlights its capacity to influence both immune responses and tissue homeostasis.

TRAF4 has associations with cancer progression and chronic inflammatory diseases. Altered expression levels of TRAF4 can contribute to tumorigenesis by affecting cell survival and proliferation involving interaction with proteins like NF-κB and TGF-beta receptors. In chronic inflammatory diseases TRAF4's dysregulation can exacerbate inflammation through pathways that involve interactions with cytokines and other TRAF family proteins. TRAF4 represents a potential target for therapeutic intervention aiming to modulate immune responses and ameliorate disease symptoms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein with E3 ligase activity that is involved in many diverse biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation or apoptosis (PubMed : 30352854, PubMed : 31076633, PubMed : 32268273, PubMed : 33991522). Promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation (PubMed : 30352854). Ubiquitinates SMURF2 through 'Lys-48'-linked ubiquitin chain leading to SMURF2 degradation through the proteasome and subsequently osteogenic differentiation (PubMed : 31076633). Promotes 'Lys-63'-mediated ubiquitination of CHK1 which in turn activates cell cycle arrest and activation of DNA repair (PubMed : 32357935). In addition, promotes an atypical 'Lys-29'-linked ubiquitination at the C-terminal end of IRS1 which is crucial for insulin-like growth factor (IGF) signal transduction (PubMed : 33991522). Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Inhibits adipogenic differentiation by activating pyruvate kinase PKM activity and subsequently the beta-catenin signaling pathway (PubMed : 32268273).

See full target information TRAF4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 296:100739 PubMed33991522

2021

The E3 ligase TRAF4 promotes IGF signaling by mediating atypical ubiquitination of IRS-1.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Yu,Ramesh Singh,Zhao Wang,Bert W O'Malley,Ping Yi

Molecular therapy. Nucleic acids 24:711-727 PubMed33996254

2021

HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Zhang,Huiqing Wen,Bangjian Peng,Jun Weng,Fanhong Zeng

Molecular medicine reports 20:1873-1882 PubMed31257517

2019

Long non‑coding RNA DLEU1 promotes cell proliferation of glioblastoma multiforme.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancun Wang,Xingyun Quan,Dingting Peng,Guancheng Hu

Journal of immunology (Baltimore, Md. : 1950) 189:33-7 PubMed22649194

2012

Cutting edge: TNF receptor-associated factor 4 restricts IL-17-mediated pathology and signaling processes.

Applications

Unspecified application

Species

Unspecified reactive species

Jarod A Zepp,Caini Liu,Wen Qian,Ling Wu,Muhammet F Gulen,Zizhen Kang,Xiaoxia Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAF4 antibody [EPR1729]

Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] (AB108991)

Western blot - Anti-TRAF4 antibody [EPR1729] (AB108991)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise