Rabbit Recombinant Monoclonal TRAF4 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (AB247736), expandable thumbnail](https://content.abcam.com/products/images/traf4-antibody-epr1729-bsa-and-azide-free-ab247736--flow-cytometry-intracellular-img139124.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (AB247736)")
![Western blot - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (AB247736), expandable thumbnail](https://content.abcam.com/products/images/traf4-antibody-epr1729-bsa-and-azide-free-ab247736--western-blot-img126073.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (AB247736)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Adapter protein with E3 ligase activity that is involved in many diverse biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation or apoptosis (PubMed:30352854, PubMed:31076633, PubMed:32268273, PubMed:33991522). Promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation (PubMed:30352854). Ubiquitinates SMURF2 through 'Lys-48'-linked ubiquitin chain leading to SMURF2 degradation through the proteasome and subsequently osteogenic differentiation (PubMed:31076633). Promotes 'Lys-63'-mediated ubiquitination of CHK1 which in turn activates cell cycle arrest and activation of DNA repair (PubMed:32357935). In addition, promotes an atypical 'Lys-29'-linked ubiquitination at the C-terminal end of IRS1 which is crucial for insulin-like growth factor (IGF) signal transduction (PubMed:33991522). Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Inhibits adipogenic differentiation by activating pyruvate kinase PKM activity and subsequently the beta-catenin signaling pathway (PubMed:32268273).
Alternative names
CART1, MLN62, RNF83, TRAF4, TNF receptor-associated factor 4, Cysteine-rich domain associated with RING and Traf domains protein 1, Metastatic lymph node gene 62 protein, RING finger protein 83, MLN 62
Recommended products
Rabbit Recombinant Monoclonal TRAF4 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1729
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247736 is the carrier-free version of Anti-TRAF4 antibody [EPR1729] ab108991.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAF4 also known as Tumor Necrosis Factor Receptor-Associated Factor 4 is a scaffold protein with a molecular weight of approximately 55 kDa. TRAF4 interacts with a variety of intracellular signaling molecules influencing signal transduction. It is expressed prominently in epithelial cells and the respiratory tract reflecting its involvement in tissue homeostasis and cell communication. The protein contains a conserved RING finger domain a novel zinc finger TRAF-type domain and a coiled-coil region which are essential for its function in protein-protein interactions.
- Biological function summary
TRAF4 participates in the regulation of immune responses and development of epithelial tissues. This protein modulates signaling pathways by promoting or inhibiting the activation of transcription factors including NF-κB and AP-1 which are critical for the immune system's function. TRAF4 associates with various adapter proteins forming signaling complexes that influence cellular outcomes during immune responses. It also plays a role in the development and integrity of the respiratory epithelium highlighting its importance in maintaining epithelial barrier functions.
- Pathways
TRAF4 integrates into the TNF receptor signaling pathway and the TGF-beta signaling pathway. In the TNF receptor pathway TRAF4 interacts with other TRAF family members mediating responses to inflammatory cytokines and regulating apoptosis. Within the TGF-beta pathway TRAF4 modulates signal transduction involving Smad proteins impacting cellular growth and differentiation. The protein's role within these pathways highlights its capacity to influence both immune responses and tissue homeostasis.
- Associated diseases and disorders
TRAF4 has associations with cancer progression and chronic inflammatory diseases. Altered expression levels of TRAF4 can contribute to tumorigenesis by affecting cell survival and proliferation involving interaction with proteins like NF-κB and TGF-beta receptors. In chronic inflammatory diseases TRAF4's dysregulation can exacerbate inflammation through pathways that involve interactions with cytokines and other TRAF family proteins. TRAF4 represents a potential target for therapeutic intervention aiming to modulate immune responses and ameliorate disease symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (ab247736), expandable thumbnail](https://content.abcam.com/products/images/traf4-antibody-epr1729-bsa-and-azide-free-ab247736--flow-cytometry-intracellular-img139124.jpg "Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (ab247736)")
Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (ab247736)
This data was developed using Anti-TRAF4 antibody [EPR1729] ab108991, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with Anti-TRAF4 antibody [EPR1729] ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-TRAF4 antibody [EPR1729] ab108991, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Western blot - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (ab247736), expandable thumbnail](https://content.abcam.com/products/images/traf4-antibody-epr1729-bsa-and-azide-free-ab247736--western-blot-img126073.jpg "Western blot - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (ab247736)")
Western blot - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (ab247736)
This data was developed using Anti-TRAF4 antibody [EPR1729] ab108991, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TRAF4 antibody [EPR1729] (Anti-TRAF4 antibody [EPR1729] ab108991) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: SKBR-3 cell lysate at 10 µg
Predicted band size: 54 kDa
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